The expert staff at NYU Langone’s Cytometry and Cell Sorting Laboratory provides researchers access to a LSRII cell analyzer with a high-throughput sampler (HTS) option. We also have a LSRII UV.

Reserve either instrument using iLab (Kerberos ID and password required).

LSRII HTS Location

NYU Langone
540 First Avenue
3rd Floor, Lab 9
Phone: 212-263-5907

LSRII HTS Optics

4 lasers and 16 parameters (see configuration)

LSRII HTS Biosafety Level

• BSL-1 work only
• nonhuman; or fixed human cells and cell lines

LSRII HTS Pricing

Self-Service

• $47 per hour: Perlmutter Cancer Center users
• $49 per hour: non–Perlmutter Cancer Center users
• $82 per hour: external academic users
• $103 per hour: external industry users

Assistance and Training

• $144 per hour: Perlmutter Cancer Center users
• $155 per hour: non–Perlmutter Cancer Center users
• $206 per hour: external academic users
• $258 per hour: external industry users

High Throughput System

• additional $10 per hour

LSRII HTS Sample Preparation

• Use only 12 × 75 mm polystyrene sample tubes (polypropylene are not compatible).
• Concentration should be 1–5 × 10⁶ cells/mL in at least 250 mL.
• Always bring extra media to dilute if necessary.
• Filter large or sticky cells.
• For HTS use, we recommend that controls (negative or single-color controls) be brought in tubes.

LSRII HTS Tutorials

LSRII user guide
FACSDiva quick start guide
FACSDiva user guide
Blank octogon and trigon templates for filter change records

LSRII HTS Configuration

Review the configuration and fluorophore list for the LSRII HTS below, or access printable versions of the configuration and filter setup.

LSRII (4 laser, 16 parameters)

LSR II HTS Filter Setup