The expert staff at NYU Langone’s Cytometry and Cell Sorting Laboratory can assist you in using a FACSAria IIu SORP cell sorter. Reserve this cell sorter using iLab (Kerberos ID and password required).

FACSAria IIu SORP Location

NYU Langone
540 First Avenue
3rd Floor, Lab 10
Phone: 212-263-5907

FACSAria IIu SORP Optics

5 lasers and 17 colors (see configuration)

FACSAria IIu SORP Biosafety Level

• BSL-2 (unscreened human material or infectious material)
• mandatory advance notice for setup and decontamination of infectious sorts
• BSL-2 standard operating procedure

FACSAria IIu SORP Pricing

• $144 per hour: Perlmutter Cancer Center users
• $155 per hour: non–Perlmutter Cancer Center users
• $206 per hour: external academic users
• $258 per hour: external industry users

FACSAria IIu SORP Sample Preparation

• Acceptable sample tubes are polypropylene 12 × 75 mm tubes, 15-mL tubes, bullet tubes, or 1.5-mL microcentrifuge tubes.
• Concentration should be 10–20 × 10⁶ cells/mL (allows for ideal pressure and best use of time).
• Always bring extra media to dilute if necessary.
• Filter all samples as close to sorting time as possible.

FACSAria IIu SORP Sample Collection

Collection tubes should be coated with appropriate media, such as FBS:

• 4 5-mL (12 × 75 mm) tubes
• 2 15-mL tubes
• 4 1.5-mL centrifuge tubes
• well plates (up to 384 wells)
• 4 “bullet” tubes (1-mL tubes)
• 4 polymerase chain reaction (PCR) tubes
• slides and cover slips

FACSAria IIu SORP Nozzle Options

70 µm

• This nozzle can sort 9 × 10⁷ total cells per hour; collection volume is ~10⁶ cells in 1 mL.
• It requires higher pressure (70 psi) and is not ideal for larger or fragile cells.

100 µm

• This nozzle can sort 5.4 × 10⁷ cells per hour; collection volume is ~4 × 10⁵ cells in 1 mL.
• It is best for large or delicate cells because it uses lower pressure (20 psi).
• Examples include stem cells, fibroblasts, neurons, and larger cancer cells.
• Thirty minutes’ extra setup time is required.

FACSAria IIu SORP Configuration

Review the configuration of the FACSAria IIu SORP below, or access a printable document.

Aria II (5 laser, 18 parameters)

Aria II Filter Setup