The Applied Bioinformatics Laboratories offer 16S ribosomal RNA (rRNA) metagenomic sequencing services, a commonly used amplicon sequencing method to identify bacterial taxonomic composition within samples and compare microbial community differences between groups.

Request a Metagenomic Sequencing Service

To request this service from us, please provide the following:

• a sample sheet with the corresponding conditions for the samples
• the link to the generated FASTQ data (obtained from NYU Langone’s Genome Technology Center or another sequencing facility)

You receive the following from us:

• a complete taxonomic table
• alpha and beta diversity analysis
• a principal component analysis plot for assessment of sample variations
• a stack bar plot of bacterial relative abundance
• box plots of bacterial relative abundance for experimental groups

Additional or Customized Analyses

We also provide the following analyses:

• LEfSe analysis
• prediction of metagenome functional content
• supervised or unsupervised clustering of differentially bacterial taxa
• correlation network analysis

 Each of these may be subject to an additional charge.

Additional Resources

Below you can find links to the computational pipelines that we typically use for our analyses:

• QIIME 2
• PICRUSt

The following select publication has used our analyses:

• Tetz G, Ruggles K, Zhou H, Heguy A, Tsirigos A, Tetz V. Bacteriophages as potential new mammalian pathogens. Scientific Reports. 2017:7, 7043.