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BELASCO LAB

 

Publications

An RNA pyrophosphohydrolase triggers 5' -exonucleolytic degradation of mRNA in Bacillus subtilis. 
Richards J, Liu Q, Pellegrini O, Celesnik H, Yao, S, Bechhofer DH, Condon C, Belasco JG
Mol. Cell. Biol. 43: 940-949 (2011)
PubMed

Ribonuclease J: how to lead a double life.
Richards J, Belasco JG
Structure 19: 1201-1203 (2011)

PubMed

All things must pass: contrasts and commonalities in eukaryotic and bacterial mRNA decay.
Belasco JG
Nature Rev. Mol. Cell Biol. 11: 467-478 (2010)

PubMed

CCR4-NOT deadenylates mRNA associated with RNA-induced silencing complexes in human cells.  
Piao X, Zhang X, Wu L, Belasco JG
Mol. Cell. Biol. 30: 1486-1494 (2010)
PubMed

A role for huntington disease protein in dendritic RNA granules.
Savas JN, Ma B, Deinhardt K, Culver BP, Restituito S, Wu L, Belasco JG, Chao MV, Tanese N
J. Biol. Chem. 285: 13142-13153 (2010)

PubMed

RNase E autoregulates its synthesis in Escherichia coli by binding directly to a stem-loop in the rne 5’ untranslated region.
Schuck A, Diwa A, Belasco JG
Mol. Microbiol. 72: 470–478 (2009)
PubMed

Structure and biological function of the RNA pyrophosphohydrolase BdRppH from Bdellovibrio bacteriovorus.
Messing SAJ, Gabelli SB, Liu Q, Celesnik H, Belasco JG, Piñeiro SA, Amzel M
Structure 17: 472-481 (2009)
PubMed


Examining the influence of microRNAs on translation efficiency and on mRNA deadenylation and decay.
Wu L, Belasco JG
Methods Enzymol
. 449: 373-393 (2008)
PubMed

PABLO analysis of RNA: 5' phosphorylation state and 5' end mapping.
Celesnik H, Deana A, Belasco JG
Methods Enzymol. 447: 83-98 (2008)
PubMed

A new window onto translational repression by bacterial sRNAs.
Richards J, Belasco JG
Mol. Cell 32: 751-753 (2008)
PubMed

Importance of translation and nonnucleolytic Ago proteins for on-target RNA interference.
Wu L, Fan J, Belasco JG
Curr. Biol. 18: 1327-1332 (2008)
PubMed

Let me count the ways: mechanisms of gene regulation by miRNAs and siRNAs.
Wu L, Belasco JG
Mol. Cell
29: 1-7 (2008)
PubMed

The bacterial enzyme RppH triggers messenger RNA degradation by 5’ pyrophosphate removal.
Deana A, Celesnik H, Belasco JG
Nature 451: 355-358 (2008)
PubMed

Initiation of RNA decay in Escherichia coli by 5’ pyrophosphate removal.
Celesnik H, Deana A, Belasco JG
Mol. Cell 27: 79-90 (2007)
PubMed

MicroRNAs direct rapid deadenylation of mRNA.
Wu L, Fan J, Belasco JG
Proc. Natl. Acad. Sci. USA 103: 4034-4039 (2006)
PubMed

Lost in translation: the influence of ribosomes on bacterial mRNA decay.
Deana A, Belasco JG
Genes Dev. 19: 2526-2533 (2005)
PubMed

MicroRNA regulation of the mammalian lin-28 gene during neuronal differentiation of embryonal carcinoma cells.
Wu L, Belasco JG
Mol. Cell. Biol. 25: 9198-9208 (2005)
PubMed

Catalytic activation of multimeric RNase E and RNase G by 5'-monophosphorylated RNA.
Jiang X, Belasco JG
Proc. Natl. Acad. Sci. USA 101: 9211-9216 (2004)
PubMed

The function of RNase G in Escherichia coli is constrained by its amino and carboxyl termini.
Deana A, Belasco JG
Mol. Microbiol. 51: 1205-1217 (2004)
PubMed

Two distinct regions on the surface of an RNA-binding domain are crucial for RNase E function.
Diwa AA, Jiang X, Schapira M, Belasco JG
Mol. Microbiol. 46(4): 959-969 (2002), Erratum in Mol. Microbiol. 47(4): 1183 (2003)
PubMed

Critical features of a conserved RNA stem-loop important for feedback regulation of RNase E synthesis.
Diwa AA, Belasco JG
J. Biol. Chem. 277: 20415-20422 (2002)
PubMed

Comparative analysis of the plant mRNA-destabilizing element, DST, in mammalian and tobacco cells.
Feldbrugge M, Aritzi P, Sullivan ML, Zamore PD, Belasco JG, Green PJ
Plant Mol. Biol. 49: 215-223 (2002)
PubMed

Consequences of RNase E scarity in Escherichia coli.
Jain C, Deana A, Belasco JG
Mol. Microbiol. 43: 1053-1064 (2002)
PubMed

T7 phage display: a novel genetic selection system for cloing RNA-binding proteins from cDNA libraries.
Danner S, Belasco JG
Proc.Natl.Acad.Sci.USA 98: 12954-12959 (2001)
PubMed

Structural model for the cooperative assembly of HIV-1 Rev multimers on the RRE as deducted from analysis of assembly-defective mutants.
Jain C, Belasco JG
Mol. Cell 7: 603-614 (2001)
PubMed

An evolutionarily conserved RNA stem-loop functions as a sensor that directs feedback regulation of RNase E gene expression.
Diwa A, Bricker AL, Jain C, Belasco JG
Genes Dev. 14:1249-1260 (2000)
PubMed

Regions of RNase E important for 5'-end-dependent RNA cleavage and autoregulated synthesis.
Jiang X, Diwa A, Belasco JG
J. Bacteriol. 182: 2468-2475 (2000)
PubMed

Rapid genetic analysis of RNA-protein interactions by translational repression in E.coli.
Jain C, Belasco JG
Methods Enzymol. 3118: 309-332 (2000)
PubMed

Importance of a 5' stem-loop for longevity of papA mRNA in Escherichia coli.
Bricker AL, Belasco JG
J. Bacteriol. 181: 3587-90 (1999)
PubMed

Target discrimination by RNA-binding proteins: role of the ancillary protein U2A' and a critical leucine residue in differentiating the RNA-binding specificity of spliceosomal proteins U1A and U2B".
Rimmele ME, Belasco JG
RNA 4: 1386-1396 (1998)
PubMed

mRNA stabilization by the ompA 5' untranslated region: two protective elements hinder distinct pathways for mRNA degradation.
Arnold TE, Yu J, Belasco JG
RNA 4: 319-330 (1998)
PubMed

RNA-binding proteins tamed.
Laird-Offringa IA, Belasco, JG
Nature Struct. Biol. 5: 665-668 (1998)
PubMed

RNA recognition by the joint action of two nucleolin RNA-binding domains: genetic analysis and structural modeling.
Bouvet P, Jain C, Belasco JG, Amalric F, Erard M
EMBO J. 16: 5235-5246 (1997)
PubMed

A rapid genetic method for the study of RNA binding proteins.
Jain C, Belasco JG
In Richter JD, ed., mRNA Formation and Function. Academic Press, 263-284 (1997)
PubMed

A structural model for the HIV-1 Rev-RRE complex deduced from altered-specificity rev variants isolated by a rapid genetic strategy.
Jain C, Belasco JG
Cell 87: 115-125. (1996)
PubMed

Translation of the adhE transcript to produce ethanol dehydrogenase requires RNase III cleavage in Escherichia coli.
Aristarkhov A, Mikulskis A, Belasco JG, Lin ECC
J. Bacteriol. 178: 4327-4332. (1996)
PubMed

In vitro genetic analysis of RNA-binding proteins using phage display libraries.
Laird-Offringa IA, Belasco JG
Methods Enzymol 267: 149-168
PubMed

Analysis of RNA-binding proteins by in vitro genetic selection: identification of an amino acid residue important for locking U1A onto its RNA target.
Laird-Offringa IA, Belasco JG
Proc. Natl. Acad. Sci. USA 92: 11859-11863 (1995)
PubMed

The nonamer UUAUUUAUU is the key AU-rich sequence motif that mediates mRNA degradation.
Zubiaga AM, Belasco JG, Greenberg ME
Mol. Cell. Biol. 15: 2219-2230 (1995)
PubMed

Autoregulation of RNase E synthesis in Escherichia coli.
Jain C, Belasco JG
Nucleic Acids Symp. Ser. 33: 85-88 (1995)
PubMed

RNase E autoregulates its synthesis by controlling the degradation rate of its own mRNA in Escherichia coli: unusual sensitivity of the rne transcript to RNase E activity.
Jain C, Belasco JG
Genes Dev 9: 84-96 (1995)
PubMed

The ompA 5' untranslated region impedes a major pathway for mRNA degradation in Escherichia coli.
Hansen MJ, Chen LH, Fejzo ML, Belasco JG
Mol Microbiol. 9: 84-96 (1995)
PubMed

Multiple elements in the c-fos protein-coding region facilitate mRNA deadenylation and decay by a mechanism coupled to translation.
Schiavi SC, Wellington CL, Shyu AB, Chen CY, Greenberg ME, Belasco JG
J. Biol. Chem. 269: 3441-3448 (1994)
PubMed

The destabilizing elements in the coding region of c-fos mRNA are recognized as RNA.
Wellington CL, Greenberg ME, Belasco JG
Mol. Cell. Biol. 5034-5042 (1993)
PubMed

Regulation of proto-oncogene mRNA stability.
Schiavi SC, Belasco JG, Greenberg ME
Biochem. Biophys. Acta 1114 : 95-106 (1992)
PubMed

Control of RNase E-mediated RNA degradation by 5'-terminal base pairing in E. coli.
Bouvet P, Belasco JG
Nature 360:488-491 (1992)
PubMed

A 5'-terminal stem-loop structure can stabilize mRNA in Escherichia coli.
Emory SA, Bouvet P, Belasco JG
Genes Dev. 6: 135-148 (1992)
PubMed

Structure and function of a bacterial mRNA stabilizer: analysis of the 5' untranslated region of ompA mRNA.
Chen LH, Emory SA, Bricker AL, Bouvet P, Belasco JG
J. Bacteriol. 173: 4758-4586 (1991)
PubMed

Two distinct destabilizing elements in the c-fos message trigger deadenylation as a first step in rapid mRNA decay.
Shyu AB, Belasco JG, Greenberg ME
Genes Dev. 172: 4578-4586 (1991)
PubMed

Degradation of pufLMX mRNA in Rhodobacter capsulatus is initiated by nonrandom endonucleolytic cleavage.
Chen CY, Belasco JG
J. Bacteriol. 172: 4578-4586 (1990)
PubMed

The ompA 5' untranslated RNA segment functions in Escherichia coli as a growth-rate-regulated mRNA stabilizer whose activity is unrelated to translational efficiency.
Emory SA, Belasco JG
J. Bacteriol. 172: 4472-4481 (1990)
PubMed

Deadenylylation: a mechanism controlling c-fos mRNA decay.
Greenberg ME, Shyu AB, Belasco JG
Enzyme 44: 181-192 (1990)
PubMed

The c-fos transcript is targeted for rapid decay by two distinct mRNA degradation pathways.
Shyu AB, Greenberg ME, Belasco JG
Genes Dev. 3: 60-72 (1989)
PubMed

Mechanism of puf mRNA degradation: the role of an intercistronic stem-loop structure.
Belasco JG, Chen CY
Genes Dev. 72: 109-117 (1988)
PubMed

Mechanisms of mRNA decay in bacteria: a perspective.
Belasco JG, Higgins CF
Gene 72: 15-23 (1988)
PubMed

An intercistronic stem-loop structure functions as an mRNA decay terminator necessary but insufficient for puf mRNA stability.
Chen CY, Beatty JT, Cohen SN, Belasco JG
Cell 52: 609-619 (1988)
PubMed

Effect of premature termination of translation on mRNA stability depends on the site of ribosome release.
Nilsson G, Belasco JG, Cohen SN, von Gabain
Proc. Natl. Acad. Sci. USA 84: 4890-4894 (1987)
PubMed

The stability of E. coli gene transcripts is dependent on determinants localized to specific mRNA segments.
Belasco JG, Nilsson G, von Gabain A, Cohen SN
Cell 46: 245-251 (1986)
PubMed

Energetics of proline racemase: rates, fractionation factors, and buffer catalysis in the oversaturated region. Nature of the interconversion of the two forms of free enzyme.
Belasco JG, Bruice TW, Fisher LM, Albery WJ, Knowles JR
Biochemistry 25: 2564-2571 (1986)
PubMed

Energetics of proline racemase: fractionation factors for the essential catalytic groups in the enzyme-substrate complexes.
Belasco JG, Bruice TW, Albery WJ, Knowles JR
Biochemistry 25: 2558-2564 (1986)
PubMed

Energetics of proline racemase: double fractionation experiment, a test for concertedness and for transition-state dominance.
Belasco JG, Albery WJ, Knowles JR
Biochemistry 25: 2552-2558 (1986)
PubMed

Energetics of proline racemase: transition-state fractionation factors for the two protons involved in the catalytic steps.
Fisher LM, Belasco JG, Bruice TW, Albery WJ, Knowles JR
Biochemistry 25: 2543-2551 (1986)
PubMed

Differential expression of photosynthesis genes in R. capsulata results from segmental differences in stability within the polycistronic rxcA transcript.
Belasco JG, Beatty JT, Adams CW, von Gabain A, Cohen SN
Cell 40: 171-181 (1985)
PubMed

Growth-rate dependent regulation of mRNA stability in Escherichia coli.
Nilsson G, Belasco JG, Cohen SN, von Gabain A
Nature 312: 75-77 (1984)
PubMed

Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts.
von Gabain A, Belasco JG, Schottel JL, Chang AC, Cohen SN
Proc. Natl. Acad. Sci. USA 80: 653-657 (1983)
PubMed

Polarization of substrate carbonyl groups by yeast aldolase: investigation by Fourier transform infrared spectroscopy.
Belasco JG, Knowles JR
Biochemistry 22: 122-129 (1983)
PubMed

Beta-lactamase proceeds via an acyl-enzyme intermediate. Interaction of the Escherichia coli RTEM enzyme with cefoxitin.
Fisher J, Belasco JG, Khosla S, Knowles JR
Biochemistry 19: 2859-2901 (1980)
PubMed

Beta-lactamase inactivation by mechanism-based reagents.
Fisher J, Belasco JG, Charnas RL, Khosla S, Knowles JR
Philos. Trans. R. Soc. Lond. B. Biol. Sci. 289: 309-319 (1980)
PubMed

Direct observation of substrate distortion by triosephosphate isomerase using Fourier transform infrared spectroscopy.
Belasco JG, Knowles JR
Biochemistry 19: 472-477 (1980)
PubMed

Critical ionization states in the reaction catalyzed by triosephosphate isomerase.
Belasco JG, Herlihy JM, Knowles JR
Biochemistry 17: 2971-2978 (1978)
PubMed


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