Applied Precision PersonalDV live-cell imaging system


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HeLa cells stained with alpha-Tubulin and Dapi. By Mathew Jones, Huang Lab, Department of Biochemistry, NYULMC  

Applied Precision PersonalDV live-cell imaging system

  • Olympus IX-71 inverted microscope
  • Wide range of objectives- 10x/0.3, 20x/0.75, 40x/1.35 oil and 60x/1.42 oil
  • Customize DeltaVision Filter Set: Standard (Dapi, Fitc, Tritc and Cy5), Live(CFP, YFP, mCherry and EGFP)
  • Uniform illumination with Xenon lamp and fiber optic module
  • Nanomotion III Precision Control stage offers the best accuracy and repeatability that are critical in sampling in a time-lapse experiment
  • DIC (20x, 40x and 60x) or phase contrast (10x) produces high contrast imags as reference to the whole cell
  • CoolSnap HQ2 CCD camera
  • Environment control unit with integrated CO2 makes cell imaging over long time
  • Restorative deconvolution is included that restores the contrast and resolution more closely represents the original sample
  • Autofocus, point-visiting, cell tracking and optical axis integration (OAI) allow live cell imaging efficient and quantified
  • Fluorescence Resonance Energy Transfer (FRET) imaging and analysis included
  • SoftWorx suite which runs under linux OS offers image viewer, 3D imaging restoration(deconvolution) and 3D visualization and analysis

 Applied Precision PersonalDV