David L. Stokes

Biosketch / Results /

David Stokes

Professor, Department of Cell Biology
Skirball Institute
Scientific Director - NYCSBE CEM

Contact Info

540-562 First Avenue
New York, NY 10016


Research Summary

CopA is an ATP-dependent Cu+ pump belonging to the family of P-type ion pumps. YiiP is a Zn2+ transporter belonging to the Cation Diffusion Facilitator (CDF) family. We used methods of helical reconstruction to solve 3D structures of both proteins. For CopA, the structures have allowed us to address the role of the N-terminal domain in regulation. Read more For YiiP, we are comparing using the structure to evaluate conformational changes related to the mechanism of transport. Read more

As part of the Transcontinental EM Initiative for Membrane Protein Structure, we have been developing technologies for producing and analyzing two-dimensional crystals. Read more

Red blood cells are characterized by a specialized cytoskeleton that confers remarkable flexibility to the cell. We have used cryo-electron tomography to visualize the 3D topology of the spectrin network that underlies this skeleton. Read more

Cardiolipin is a specialized lipid found exclusively in the inner membrane of mitochondria. We have used electron tomography to characterize morphological defects to mitochondrial cristae as well as abnormalities in the distribution of ATP synthase caused by a deficit in cardiolipin that leads to Barth's syndrome. Read more

The adaptive immune response is initiated when naive T-cells recognize a specific antigen on the surface of an antigen presenting cell. We have been studying this process using electron tomography and have discovered a surprising release of vesicles at a specialized zone at the intercellular interface. Read more

Phi12 is a bacteriophage belonging to the family of cystoviridae. We have used cryo-electron tomography and sub-tomogram averaging to analyze the 3D structure of the surface glycoprotein responsible for host recognition and infection. Read more

Research Interests

Transmembrane Ion Transport and Structure of Cellular Assemblies by Electron Crystallography and Electron Tomography

Research Keywords

cryo-electron microscopy, X-ray crystallography, ion transport

Sparse and incomplete factorial matrices to screen membrane protein 2D crystallization
Lasala, R; Coudray, N; Abdine, A; Zhang, Z; Lopez-Redondo, M; Kirshenbaum, R; Alexopoulos, J; Zolnai, Z; Stokes, D L; Ubarretxena-Belandia, I
2015-02-10; 1047-8477,Journal of structural biology - id: 1457402, year: 2015 Journal Article

Polarized release of T-cell-receptor-enriched microvesicles at the immunological synapse
Choudhuri, Kaushik; Llodra, Jaime; Roth, Eric W; Tsai, Jones; Gordo, Susana; Wucherpfennig, Kai W; Kam, Lance C; Stokes, David L; Dustin, Michael L
2014-03-03; 0028-0836,Nature - id: 820872, year: 2014 JOURNAL ARTICLE

Two-dimensional crystallization of membrane proteins: Screening strategies
Coudray, N; Lasala, R; Zhang, Z; Zolnai, Z; Ubarretxena, I; Stokes, D
2015-06-01; 1431-9276,Microscopy & microanalysis - id: 1605972, year: 2014

Modeling, Docking, and Fitting of Atomic Structures to 3D Maps from Cryo-Electron Microscopy
Allen, Gregory S; Stokes, David L
2012-12-17; 1064-3745,Methods in molecular biology - id: 202212, year: 2013 Journal Article

Three-dimensional reconstruction of intact human integrin alphaIIbbeta3: new implications for activation-dependent ligand binding
Choi, Won-Seok; Rice, William J; Stokes, David L; Coller, Barry S
2014-02-19; 0006-4971,Blood - id: 808132, year: 2013 Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't