Tony T. Huang

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Tony Huang

Associate Professor, Department of Biochemistry and Molecular Pharmacology
Module Dir for Protein Modifications of Disease
Graduate Advisor for Molecular Oncology and Tumor Immunology Training Program

Contact Info

Address
550 First Avenue
New York, NY 10016

212/263-9046
Tony.Huang@nyumc.org

Research Summary

Ubiquitination is a highly regulated process conserved in all eukaryotes that controls a broad range of cellular functions, from proteolysis, signal transduction, endocytosis, to DNA repair and genomic stability. Like phosphorylation, ubiquitination can be a dynamic and reversible post-translational process whereby an enzyme cascade conjugates ubiquitin to a target protein, and a family of proteases, the deubiquitinating enzymes (DUBs), are potent at removing this modification. Our lab studies two important DNA damage response pathways that are "switched on" by non-proteolytic ubiquitination events: the Fanconi Anemia (FA) DNA cross-link repair and genome stability pathway, and the DNA damage tolerance translesion synthesis pathway (TLS). While the elucidation of upstream activators of diverse DNA damage response pathways remains to be the focus of the field, signal transduction events that "switch off" DNA repair have been largely ignored. To begin addressing this issue, a major focus of my lab will be to study the action of DUBs in the context of DNA repair pathways. In humans, protein deubiquitination is controlled by a family of over 90 distinct DUB enzymes, of which the majority has not been functionally characterized. We utilized a gene family-specific siRNA library to identify DUBs that negatively regulate different aspects of DNA repair and genome stability pathways in human cells. Research projects in my lab will include the characterization and elucidation of signaling mechanisms surrounding DUB activation and inactivation and how these molecular events function to confer genomic stability in normal versus cancerous cells.

Research Interests

Regulation of Ubiquitination and Deubiquitination in DNA repair and cancer susceptibility pathways

Recognition of Lys48-Linked Di-ubiquitin and Deubiquitinating Activities of the SARS Coronavirus Papain-like Protease
Bekes, Miklos; van der Heden van Noort, Gerbrand J; Ekkebus, Reggy; Ovaa, Huib; Huang, Tony T; Lima, Christopher D. Recognition of Lys48-Linked Di-ubiquitin and Deubiquitinating Activities of the SARS Coronavirus Papain-like Protease. Molecular cell. 2016 May;62(4):572-585 (2112432)

How SUMOylation Fine-Tunes the Fanconi Anemia DNA Repair Pathway
Coleman, Kate E; Huang, Tony T. How SUMOylation Fine-Tunes the Fanconi Anemia DNA Repair Pathway. Frontiers in genetics. 2016 ;7:61-61 (2100902)

HUWE1 comes to the rescue at stalled replication forks
Coleman, Kate E; Huang, Tony T. HUWE1 comes to the rescue at stalled replication forks. EMBO reports. 2016 May 4;17(6):781-782 (2101252)

ATR-Mediated Phosphorylation of FANCI Regulates Dormant Origin Firing in Response to Replication Stress
Chen, Yu-Hung; Jones, Mathew J K; Yin, Yandong; Crist, Sarah B; Colnaghi, Luca; Sims, Robert J 3rd; Rothenberg, Eli; Jallepalli, Prasad V; Huang, Tony T. ATR-Mediated Phosphorylation of FANCI Regulates Dormant Origin Firing in Response to Replication Stress. Molecular cell. 2015 Apr 2;58(2):323-338 (1521972)

The Role of Deubiquitinating Enzymes in DNA Repair
Kee, Younghoon; Huang, Tony T. The Role of Deubiquitinating Enzymes in DNA Repair. Molecular & cellular biology. 2015 Dec 7;36(4):524-544 (1870052)