Tony T. Huang

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Tony Huang

Associate Professor, Department of Biochemistry and Molecular Pharmacology
Biochemistry and Molecular Pharmacology
Grad Adv Molecular Oncology & Tumor Imm Train Pgm, Module Dir for Protein Modifications of Disease

Contact Info

550 First Avenue
New York, NY 10016


Research Summary

Ubiquitination is a highly regulated process conserved in all eukaryotes that controls a broad range of cellular functions, from proteolysis, signal transduction, endocytosis, to DNA repair and genomic stability. Like phosphorylation, ubiquitination can be a dynamic and reversible post-translational process whereby an enzyme cascade conjugates ubiquitin to a target protein, and a family of proteases, the deubiquitinating enzymes (DUBs), are potent at removing this modification. Our lab studies two important DNA damage response pathways that are "switched on" by non-proteolytic ubiquitination events: the Fanconi Anemia (FA) DNA cross-link repair and genome stability pathway, and the DNA damage tolerance translesion synthesis pathway (TLS). While the elucidation of upstream activators of diverse DNA damage response pathways remains to be the focus of the field, signal transduction events that "switch off" DNA repair have been largely ignored. To begin addressing this issue, a major focus of my lab will be to study the action of DUBs in the context of DNA repair pathways. In humans, protein deubiquitination is controlled by a family of over 90 distinct DUB enzymes, of which the majority has not been functionally characterized. We utilized a gene family-specific siRNA library to identify DUBs that negatively regulate different aspects of DNA repair and genome stability pathways in human cells. Research projects in my lab will include the characterization and elucidation of signaling mechanisms surrounding DUB activation and inactivation and how these molecular events function to confer genomic stability in normal versus cancerous cells.

Research Interests

Regulation of Ubiquitination and Deubiquitination in DNA repair and cancer susceptibility pathways

ATR-Mediated Phosphorylation of FANCI Regulates Dormant Origin Firing in Response to Replication Stress
Chen, Yu-Hung; Jones, Mathew J K; Yin, Yandong; Crist, Sarah B; Colnaghi, Luca; Sims, Robert J 3rd; Rothenberg, Eli; Jallepalli, Prasad V; Huang, Tony T
2015-04-09; 1097-4164,Molecular cell - id: 1521972, year: 2015 JOURNAL ARTICLE

Co-opting the Fanconi Anemia Genomic Stability Pathway Enables Herpesvirus DNA Synthesis and Productive Growth
Karttunen, Heidi; Savas, Jeffrey N; McKinney, Caleb; Chen, Yu-Hung; Yates, John R 3rd; Hukkanen, Veijo; Huang, Tony T; Mohr, Ian
2014-07-01; 1097-2765,Molecular cell - id: 1055622, year: 2014 JOURNAL ARTICLE

DUB-Resistant Ubiquitin to Survey Ubiquitination Switches in Mammalian Cells
Bekes, Miklos; Okamoto, Keiji; Crist, Sarah B; Jones, Mathew J; Chapman, Jessica R; Brasher, Bradley B; Melandri, Francesco D; Ueberheide, Beatrix M; Lazzerini Denchi, Eros; Huang, Tony T
2013-11-13; 2211-1247,Cell reports - id: 617482, year: 2013 JOURNAL ARTICLE

Deubiquitinases as a Signaling Target of Oxidative Stress
Cotto-Rios, Xiomaris M; Bekes, Miklos; Chapman, Jessica; Ueberheide, Beatrix; Huang, Tony T
2012-12-25; 2211-1247,Cell reports - id: 204152, year: 2012 JOURNAL ARTICLE

Dysregulation of DNA polymerase recruitment to replication forks results in genomic instability
Jones, M J K; Colnaghi, L; Huang, T T
2012-03-06; 0261-4189,EMBO journal - id: 158609, year: 2012