Tony T. Huang

Biosketch / Results /

Tony T. Huang, Ph.D.

; Co Course Director
Department of Biochemistry and Molecular Pharmacology

Contact Info

Address
550 First Avenue
NYU medical School Floor Floor 3 Room 399
Medical Science Building
New York, NY 10016

212-263-9046
Tony.Huang@nyumc.org

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Education

2003-2006 — Dana Farber Cancer Institute, Harvard Med. School, PostDoctoral Training
— University of Wisconsin, Graduate Education

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Research Summary

Ubiquitination is a highly regulated process conserved in all eukaryotes that controls a broad range of cellular functions, from proteolysis, signal transduction, endocytosis, to DNA repair and genomic stability. Like phosphorylation, ubiquitination can be a dynamic and reversible post-translational process whereby an enzyme cascade conjugates ubiquitin to a target protein, and a family of proteases, the deubiquitinating enzymes (DUBs), are potent at removing this modification. Our lab studies two important DNA damage response pathways that are "switched on" by non-proteolytic ubiquitination events: the Fanconi Anemia (FA) DNA cross-link repair and genome stability pathway, and the DNA damage tolerance translesion synthesis pathway (TLS). While the elucidation of upstream activators of diverse DNA damage response pathways remains to be the focus of the field, signal transduction events that "switch off" DNA repair have been largely ignored. To begin addressing this issue, a major focus of my lab will be to study the action of DUBs in the context of DNA repair pathways. In humans, protein deubiquitination is controlled by a family of over 90 distinct DUB enzymes, of which the majority has not been functionally characterized. We utilized a gene family-specific siRNA library to identify DUBs that negatively regulate different aspects of DNA repair and genome stability pathways in human cells. Research projects in my lab will include the characterization and elucidation of signaling mechanisms surrounding DUB activation and inactivation and how these molecular events function to confer genomic stability in normal versus cancerous cells.

Research Interests

Regulation of Ubiquitination and Deubiquitination in DNA repair and cancer susceptibility pathways

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All data from NYU Health Sciences Library Faculty Bibliography — -

Contact:
http://hsl.med.nyu.edu/faculty-bibliography-search

Co-opting the Fanconi Anemia Genomic Stability Pathway Enables Herpesvirus DNA Synthesis and Productive Growth
Karttunen, Heidi; Savas, Jeffrey N; McKinney, Caleb; Chen, Yu-Hung; Yates, John R 3rd; Hukkanen, Veijo; Huang, Tony T; Mohr, Ian
2014 Jun;:111-122, Molecular cell
— id: 1055622, year: 2014, vol: , page: 111, stat: Journal Article,

DUB-Resistant Ubiquitin to Survey Ubiquitination Switches in Mammalian Cells
Bekes, Miklos; Okamoto, Keiji; Crist, Sarah B; Jones, Mathew J; Chapman, Jessica R; Brasher, Bradley B; Melandri, Francesco D; Ueberheide, Beatrix M; Lazzerini Denchi, Eros; Huang, Tony T
2013 Nov;:826-838, Cell reports
— id: 617482, year: 2013, vol: , page: 826, stat: Journal Article,

Deubiquitinases as a Signaling Target of Oxidative Stress
Cotto-Rios, Xiomaris M; Bekes, Miklos; Chapman, Jessica; Ueberheide, Beatrix; Huang, Tony T
2012 Dec;:1475-1484, Cell reports
— id: 204152, year: 2012, vol: , page: 1475, stat: Journal Article,

Dysregulation of DNA polymerase recruitment to replication forks results in genomic instability
Jones, M J K; Colnaghi, L; Huang, T T
2012 15 Feb 2012;31(4):908-918, EMBO journal
— id: 158609, year: 2012, vol: 31, page: 908, stat: Journal Article,

The Fanconi anemia pathway in replication stress and DNA crosslink repair
Jones, Mathew J. K.; Huang, Tony T.
2012 DEC;69(23):3963-3974, Cellular & molecular life sciences: CMLS
— id: 185712, year: 2012, vol: 69, page: 3963, stat: Journal Article,

The Auto-Generated Fragment of the Usp1 Deubiquitylase Is a Physiological Substrate of the N-End Rule Pathway
Piatkov, Konstantin I; Colnaghi, Luca; Bekes, Miklos; Varshavsky, Alexander; Huang, Tony T
2012 Nov;:926-933, Molecular cell
— id: 204162, year: 2012, vol: , page: 926, stat: Journal Article,

Patient-derived C-terminal mutation of FANCI causes protein mislocalization and reveals putative EDGE motif function in DNA repair
Colnaghi, Luca; Jones, Mathew J K; Cotto-Rios, Xiomaris M; Schindler, Detlev; Hanenberg, Helmut; Huang, Tony T
2011 Feb 17;117(7):2247-2256, Blood
— id: 124093, year: 2011, vol: 117, page: 2247, stat: Journal Article,

Insights into phosphorylation-dependent mechanisms regulating USP1 protein stability during the cell cycle
Cotto Rios XM; Jones MJ; Huang TT
2011 Dec 1;10(23):4009-4016, Cell cycle
— id: 145997, year: 2011, vol: 10, page: 4009, stat: Journal Article,

APC/CCdh1-dependent proteolysis of USP1 regulates the response to UV-mediated DNA damage
Cotto-Rios, Xiomaris M; Jones, Mathew J K; Busino, Luca; Pagano, Michele; Huang, Tony T
2011 Jul 25;194(2):177-186, Journal of cell biology
— id: 135576, year: 2011, vol: 194, page: 177, stat: Journal Article,

Nuclear export of the NF-kappaB inhibitor IkappaBalpha is required for proper B cell and secondary lymphoid tissue formation
Wuerzberger-Davis, Shelly M; Chen, Yuhong; Yang, David T; Kearns, Jeffrey D; Bates, Paul W; Lynch, Candace; Ladell, Nicholas C; Yu, Mei; Podd, Andrew; Zeng, Hu; Huang, Tony T; Wen, Renren; Hoffmann, Alexander; Wang, Demin; Miyamoto, Shigeki
2011 Feb 25;34(2):188-200, Immunity
— id: 130274, year: 2011, vol: 34, page: 188, stat: Journal Article,

Calcium-dependent regulation of NEMO nuclear export in response to genotoxic stimuli
Berchtold, Craig M; Wu, Zhao-Hui; Huang, Tony T; Miyamoto, Shigeki
2007 Jan;27(2):497-509, Molecular & cellular biology
— id: 69204, year: 2007, vol: 27, page: 497, stat: Journal Article,

A UAF1-containing multisubunit protein complex regulates the Fanconi anemia pathway
Cohn, Martin A; Kowal, Przemyslaw; Yang, Kailin; Haas, Wilhelm; Huang, Tony T; Gygi, Steven P; D'Andrea, Alan D
2007 Dec 14;28(5):786-797, Molecular cell
— id: 130275, year: 2007, vol: 28, page: 786, stat: Journal Article,

FANCI is a second monoubiquitinated member of the Fanconi anemia pathway
Sims, Ashley E; Spiteri, Elizabeth; Sims, Robert J 3rd; Arita, Adriana G; Lach, Francis P; Landers, Thomas; Wurm, Melanie; Freund, Marcel; Neveling, Kornelia; Hanenberg, Helmut; Auerbach, Arleen D; Huang, Tony T
2007 Jun;14(6):564-567, Nature structural & molecular biology
— id: 72707, year: 2007, vol: 14, page: 564, stat: Journal Article,

HAUSP hunting the FOX(O)
Huang, Tony T; D'Andrea, Alan D
2006 Oct;8(10):1043-1045, Nature cell biology
— id: 69205, year: 2006, vol: 8, page: 1043, stat: Journal Article,

Regulation of DNA repair by ubiquitylation
Huang, Tony T; D'Andrea, Alan D
2006 May;7(5):323-334, Nature reviews. Molecular cell biology
— id: 69206, year: 2006, vol: 7, page: 323, stat: Journal Article,

Regulation of monoubiquitinated PCNA by DUB autocleavage
Huang, Tony T; Nijman, Sebastian M B; Mirchandani, Kanchan D; Galardy, Paul J; Cohn, Martin A; Haas, Wilhelm; Gygi, Steven P; Ploegh, Hidde L; Bernards, Rene; D'Andrea, Alan D
2006 Apr;8(4):339-347, Nature cell biology
— id: 69207, year: 2006, vol: 8, page: 339, stat: Journal Article,

The deubiquitinating enzyme USP1 regulates the Fanconi anemia pathway
Nijman, Sebastian M B; Huang, Tony T; Dirac, Annette M G; Brummelkamp, Thijn R; Kerkhoven, Ron M; D'Andrea, Alan D; Bernards, Rene
2005 Feb 4;17(3):331-339, Molecular cell
— id: 69208, year: 2005, vol: 17, page: 331, stat: Journal Article,

Sequential modification of NEMO/IKKgamma by SUMO-1 and ubiquitin mediates NF-kappaB activation by genotoxic stress
Huang, Tony T; Wuerzberger-Davis, Shelly M; Wu, Zhao-Hui; Miyamoto, Shigeki
2003 Nov 26;115(5):565-576, Cell
— id: 69209, year: 2003, vol: 115, page: 565, stat: Journal Article,

The zinc finger domain of NEMO is selectively required for NF-kappa B activation by UV radiation and topoisomerase inhibitors
Huang, Tony T; Feinberg, Shelby L; Suryanarayanan, Sainath; Miyamoto, Shigeki
2002 Aug;22(16):5813-5825, Molecular & cellular biology
— id: 69210, year: 2002, vol: 22, page: 5813, stat: Journal Article,

Postrepression activation of NF-kappaB requires the amino-terminal nuclear export signal specific to IkappaBalpha
Huang, T T; Miyamoto, S
2001 Jul;21(14):4737-4747, Molecular & cellular biology
— id: 130276, year: 2001, vol: 21, page: 4737, stat: Journal Article,

A nuclear export signal in the N-terminal regulatory domain of IkappaBalpha controls cytoplasmic localization of inactive NF-kappaB/IkappaBalpha complexes
Huang, T T; Kudo, N; Yoshida, M; Miyamoto, S
2000 Feb 1;97(3):1014-1019, Proceedings of the National Academy of Sciences of the United States of America
— id: 130279, year: 2000, vol: 97, page: 1014, stat: Journal Article,

NF-kappaB activation by camptothecin. A linkage between nuclear DNA damage and cytoplasmic signaling events
Huang, T T; Wuerzberger-Davis, S M; Seufzer, B J; Shumway, S D; Kurama, T; Boothman, D A; Miyamoto, S
2000 Mar 31;275(13):9501-9509, Journal of biological chemistry
— id: 130278, year: 2000, vol: 275, page: 9501, stat: Journal Article,

Cellular and molecular responses to topoisomerase I poisons. Exploiting synergy for improved radiotherapy
Miyamoto, S; Huang, T T; Wuerzberger-Davis, S; Bornmann, W G; Pink, J J; Tagliarino, C; Kinsella, T J; Boothman, D A
2000 ;922:274-292, Annals of the New York Academy of Sciences
— id: 130277, year: 2000, vol: 922, page: 274, stat: Journal Article,