Biosketch / Results /
Leslie GoldAssociate Professor, Department of Medicine;Associate Professor, Department of Pathology
462 First Avenue
New York, NY 10016
Education— NYU, Medical Education
The laboratory studies loss of growth regulation in cancer, particularly hormone-regulated cancers. Specific objectives emphasize alterations in cancer cells with respect to the regulation of cell cycle proteins by ubiquitin-mediated degradation, nuclear-cytoplasmic shuttling of cell cycle proteins, growth factor signaling pathways, particularly TGF-β, hormone-regulated growth, and stromal/epithelial interactions. For example, we are the first to show that TGF-β, estrogen, and progesterone control endometrial epithelial cell proliferation by regulating the protein levels of the cyclin-dependent kinase inhibitor, p27kip1 (p27) through the ubiquitin pathway (Diagram). Therefore, identifying specific inhibitors of the E3 ligase, Skp2 to potentially prevent p27 degradation to regain growth control is a potential specific therapy for endometrial carcinoma and other human cancers showing loss of nuclear p27. We utilize epithelial and stromal cells, isolated from normal and malignant endometrium, in primary cultures, co-cultures, and 3-dimensional matrices as our major model systems. These physiological paradigms afford optimal approaches to understanding the role of stromal cells surrounding malignant glands in malignant progression (tumor microenvironment) and a greater probability for defining molecular targets for translation into novel treatments for cancer prevention and therapy.
We discovered that the intracellular calcium-binding endoplasmic reticulum (ER) chaperone protein, calreticulin has non-ER functions of topically enhancing the rate and quality of wound healing and exogenously, inducing proliferation, migration, and matrix protein induction of cells involved in the wound healing process. The mechanisms involved in how calreticulin induces these functions from the outside-inward and the receptors are unknown opening a new area for the lab to explore.
Permanent member of Metabolic Pathology and Tumor Cell Biology IRGs, NIH; Council, American Association Cancer Research (WICR), SEP Review Panel for Gynecological SPORE, NIH; Review Panel for AACR Fellowships, Ad hoc Reviewer for numerous IRGs, NIH; Associate Editor, Molecular Signaling.
Research InterestsTranslational medicine, hormonal carcinogenesis, normal and malignant growth regulatory mechanisms, post-translational protein modifications, cell cycle, ubiquitin pathway regulation of growth, TGF-β signaling, stromal/epithelial interactions and cell substrate interactions in cancer progression, molecular targets for cancer prevention and therapy, wound/tissue repair, developing physiological model systems.
High-level secretion of native recombinant human calreticulin in yeast
Ciplys, Evaldas; Zitkus, Eimantas; Gold, Leslie I; Daubriac, Julien; Pavlides, Savvas C; Hojrup, Peter; Houen, Gunnar; Wang, Wen-An; Michalak, Marek; Slibinskas, Rimantas. High-level secretion of native recombinant human calreticulin in yeast. Microbial cell factories. 2015 ;14(1):165-165 (1803742)
Unfolding the complexities of ER chaperones in health and disease: report on the 11th international calreticulin workshop
Gold, Leslie; Williams, David; Groenendyk, Jody; Michalak, Marek; Eggleton, Paul. Unfolding the complexities of ER chaperones in health and disease: report on the 11th international calreticulin workshop. Cell stress chaperones. 2015 Nov;20(6):875-883 (1812692)
A new role for the multifunctionalwound healing agent calreticulin (CRT) in combating infection: Significance for treating diabetic foot ulcers
Gold, L I; Pavlides, S C; Ojeda, J; Eaton, B; Panchal, R G. A new role for the multifunctionalwound healing agent calreticulin (CRT) in combating infection: Significance for treating diabetic foot ulcers [Meeting Abstract]. Wound repair & regeneration. 2014 March- April;22(2):A42-A42 (1361312)
Binding Of Apoptotic Fetal Cardiocytes By Anti-Ro Antibodies Stimulates uPA/uPAR-Dependent Macrophage Infiltration and M2 Type Phenotype
Briasouli, Paraskevi ; Pavlides, Savvas ; Gold, Leslie ; Halushka, Mark ; Buyon, Jill P.. Binding Of Apoptotic Fetal Cardiocytes By Anti-Ro Antibodies Stimulates uPA/uPAR-Dependent Macrophage Infiltration and M2 Type Phenotype [Meeting Abstract]. Arthritis & rheumatism. 2013 OCT;65 10 10(SI):S750-S750 (657512)
Exogenous calreticulin improves diabetic wound healing (vol 20, pg 715, 2012)
Greives, Matthew R.; Samra, Fares; Pavlides, Savvas C.; Blechman, Keith M.; Naylor, Sara-Megumi; Woodrell, Christopher D.; Cadacio, Caprice; Levine, Jamie P.; Asoulin, Tara A.; Michalak, Marek; Warren, Stephen M.; Gold, Leslie I.. Exogenous calreticulin improves diabetic wound healing (vol 20, pg 715, 2012). Wound repair & regeneration. 2013 MAR-APR;21(2):337-337 (288862)