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Joel G. Belasco

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Joel G. Belasco, Ph.D.

Professor; Coord Structural Biology Program
Department of Microbiology (Skirball) and Structural Biology

Contact Info

Address
540 First Avenue
Floor 3 Room Labs 2 & 3
Skirball Institute
New York, NY 10016

Phone: 212-263-5409
Fax: 212-263-8951
Email: Joel.Belasco@med.nyu.edu

Web:	Dr. Belasco's laboratory website

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Education

— Dr. Belasco received his Ph.D. in Chemistry from Harvard in 1980., Graduate Education
— After a year of postdoctoral studies in the Department of Chemistry at Harvard (1980-1981), he moved to the Department of Genetics at Stanford University to complete his postdoctoral training (1981-1985)., PostDoctoral Training

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Research Summary

Post-transcriptional processes play a crucial role in controlling gene expression in all organisms. Our research is aimed at elucidating the molecular mechanisms by which such control is imposed. We are particularly interested in two important means by which genes are regulated post-transcriptionally: messenger RNA degradation and repression by microRNAs and siRNAs. The goal of our investigations is to identify and characterize the proteins, RNA elements, and molecular mechanisms that govern these key regulatory processes in bacterial and mammalian cells.

mRNA DEGRADATION

5'-end dependence. In bacteria, the lifetimes of mRNAs can differ by more than an order of magnitude, with profound consequences for gene expression. For many years it had been assumed that mRNA degradation in E. coli begins with endonucleolytic cleavage at internal sites. However, our recent findings have challenged that view by showing that mRNA decay can instead be triggered by a prior non-nucleolytic event that marks transcripts for rapid turnover: the rate-determining conversion of the 5' terminus from a triphosphate to a monophosphate. This modification creates better substrates for the endonuclease RNase E, whose cleavage activity is greatly enhanced when the RNA 5' end is monophosphorylated. We have identified the pyrophosphate-removing hydrolase responsible for that 5'-terminal event, the first such bacterial enzyme ever characterized. That the action of the pyrophosphohydrolase is impeded when the 5' end is structurally sequestered by a stem-loop helps to explain the stabilizing influence of 5'-terminal base pairing on mRNA lifetimes in vivo. Interestingly, this master regulator of 5'-end-dependent mRNA degradation in E. coli not only catalyzes a process functionally reminiscent of eukaryotic mRNA decapping but also bears an evolutionary relationship to the eukaryotic decapping enzyme Dcp2.

RNase E autoregulation. To ensure a steady supply of RNase E, E. coli and related bacteria have evolved a homeostatic mechanism for tightly regulating the synthesis of that important enzyme by modulating the decay rate of rne (RNase E) mRNA in response to changes in cellular RNase E activity. We have determined the secondary structure of the rne 5' untranslated region and identified the elements within it that function in cis to mediate feedback regulation by RNase E. In vitro studies with purified components indicate that these 5' UTR elements, like a 5' monophosphate, act directly to expedite RNA degradation by binding to RNase E and guiding it to nearby cleavage sites.

MicroRNA FUNCTION

Human cells contain hundreds of different microRNAs, short RNA molecules that function as negative genetic regulators. In animal cells, microRNAs act by annealing to mRNAs to which they are imperfectly complementary. Our studies have shown that microRNAs inhibit gene expression not only by repressing translation but also by directing rapid poly(A) tail removal, thereby hastening mRNA degradation. The ability of microRNAs to expedite deadenylation does not result from decreased translation; nor does translational repression by microRNAs require a poly(A) tail. These findings suggest that microRNAs utilize two distinct post-transcriptional mechanisms to downregulate gene expression.

Small interfering RNAs (siRNAs), the mediators of RNA interference, are closely related to microRNAs. Although siRNAs were originally thought to inhibit the function of fully complementary messages solely by guiding endonucleolytic cleavage, our recent data indicate that they can also repress translation of those messages. In addition, we have found that the specificity of RNA interference by siRNAs is cell-type-dependent due to disparities in the tissue distribution and activity of the four Ago proteins that deliver siRNAs to their mRNA targets in human cells.

Research Interests

Post-transcriptional gene regulation, including control of mRNA degradation and mechanisms of microRNA function.

Research Keywords

gene expression, post transcriptional regulation, mRNA stability, RNA, RNA-binding proteins, molecular recognition

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All data from NYU Health Sciences Library Faculty Bibliography — -

Contact:
http://hsl.med.nyu.edu/faculty-bibliography-search#about

Ribonuclease j: how to lead a double life
Richards, Jamie; Belasco, Joel G
2011 Sep 7;19(9):1201-1203, Structure
— id: 137079, year: 2011, vol: 19, page: 1201, stat: Journal Article,

An RNA Pyrophosphohydrolase Triggers 5'-Exonucleolytic Degradation of mRNA in Bacillus subtilis
Richards, Jamie; Liu, Quansheng; Pellegrini, Olivier; Celesnik, Helena; Yao, Shiyi; Bechhofer, David H; Condon, Ciaran; Belasco, Joel G
2011 Sep 16;43(6):940-949, Molecular cell
— id: 137849, year: 2011, vol: 43, page: 940, stat: Journal Article,

Decay of a model mRNA in Bacillus subtilis by a combination of RNase J1 5' exonuclease and RNase Y endonuclease activities [Note]
Yao S.; Richards J.; Belasco J.G.; Bechhofer D.H.
2011 ;193(22):6384-6386, Journal of bacteriology
— id: 141499, year: 2011, vol: 193, page: 6384, stat: Journal Article,

All things must pass: contrasts and commonalities in eukaryotic and bacterial mRNA decay
Belasco, Joel G
2010 Jul;11(7):467-478, Nature reviews. Molecular cell biology
— id: 110080, year: 2010, vol: 11, page: 467, stat: Journal Article,

CCR4-NOT deadenylates RISC-associated mRNA in human cells
Piao, Xianghua; Zhang, Xue; Wu, Ligang; Belasco, Joel G
2010 Mar;30(6):1486-1494, Molecular & cellular biology
— id: 106565, year: 2010, vol: 30, page: 1486, stat: Journal Article,

A role for huntington disease protein in dendritic RNA granules
Savas, Jeffrey N; Ma, Bin; Deinhardt, Katrin; Culver, Brady P; Restituito, Sophie; Wu, Ligang; Belasco, Joel G; Chao, Moses V; Tanese, Naoko
2010 Apr 23;285(17):13142-13153, Journal of biological chemistry
— id: 109209, year: 2010, vol: 285, page: 13142, stat: Journal Article,

Structure and Biological Function of the RNA Pyrophosphohydrolase BdRppH from Bdellovibrio bacteriovorus
Messing, Simon A J; Gabelli, Sandra B; Liu, Quansheng; Celesnik, Helena; Belasco, Joel G; Pineiro, Silvia A; Amzel, L Mario
2009 Mar 11;17(3):472-481, Structure
— id: 95121, year: 2009, vol: 17, page: 472, stat: Journal Article,

RNase E autoregulates its synthesis in Escherichia coli by binding directly to a stem-loop in the rne 5' untranslated region
Schuck, Alyssa; Diwa, Alexis; Belasco, Joel G
2009 Apr;72(2):470-478, Molecular microbiology
— id: 98002, year: 2009, vol: 72, page: 470, stat: Journal Article,

PABLO analysis of RNA 5'-phosphorylation state and 5'-end mapping
Celesnik, Helena; Deana, Atilio; Belasco, Joel G
2008 ;447:83-98, Methods in enzymology
— id: 93571, year: 2008, vol: 447, page: 83, stat: Journal Article,

The bacterial enzyme RppH triggers messenger RNA degradation by 5' pyrophosphate removal
Deana, Atilio; Celesnik, Helena; Belasco, Joel G
2008 Jan 17;451(7176):355-358, Nature
— id: 76115, year: 2008, vol: 451, page: 355, stat: Journal Article,

A new window onto translational repression by bacterial sRNAs
Richards, Jamie; Belasco, Joel G
2008 Dec 26;32(6):751-753, Molecular cell
— id: 91984, year: 2008, vol: 32, page: 751, stat: Journal Article,

Chapter 18. Examining the influence of microRNAs on translation efficiency and on mRNA deadenylation and decay
Wu, Ligang; Belasco, Joel G
2008 ;449:373-393, Methods in enzymology
— id: 106002, year: 2008, vol: 449, page: 373, stat: Journal Article,

Let me count the ways: mechanisms of gene regulation by miRNAs and siRNAs
Wu, Ligang; Belasco, Joel G
2008 Jan 18;29(1):1-7, Molecular cell
— id: 76460, year: 2008, vol: 29, page: 1, stat: Journal Article,

Importance of translation and nonnucleolytic ago proteins for on-target RNA interference
Wu, Ligang; Fan, Jihua; Belasco, Joel G
2008 Sep 9;18(17):1327-1332, Current biology. CB
— id: 93365, year: 2008, vol: 18, page: 1327, stat: Journal Article,

Initiation of RNA decay in Escherichia coli by 5' pyrophosphate removal
Celesnik, Helena; Deana, Atilio; Belasco, Joel G
2007 Jul 6;27(1):79-90, Molecular cell
— id: 73302, year: 2007, vol: 27, page: 79, stat: Journal Article,

MicroRNAs direct rapid deadenylation of mRNA
Wu, Ligang; Fan, Jihua; Belasco, Joel G
2006 Mar 14;103(11):4034-4039, Proceedings of the National Academy of Sciences of the United States of America
— id: 63836, year: 2006, vol: 103, page: 4034, stat: Journal Article,

Lost in translation: the influence of ribosomes on bacterial mRNA decay
Deana, Atilio; Belasco, Joel G
2005 Nov 1;19(21):2526-2533, Genes & development
— id: 61339, year: 2005, vol: 19, page: 2526, stat: Journal Article,

Micro-RNA regulation of the mammalian lin-28 gene during neuronal differentiation of embryonal carcinoma cells
Wu, Ligang; Belasco, Joel G
2005 Nov;25(21):9198-9208, Molecular & cellular biology
— id: 61268, year: 2005, vol: 25, page: 9198, stat: Journal Article,

The function of RNase G in Escherichia coli is constrained by its amino and carboxyl termini
Deana, Atilio; Belasco, Joel G
2004 Mar;51(4):1205-1217, Molecular microbiology
— id: 46028, year: 2004, vol: 51, page: 1205, stat: Journal Article,

Catalytic activation of multimeric RNase E and RNase G by 5'-monophosphorylated RNA
Jiang, Xunqing; Belasco, Joel G
2004 Jul 22;101(25):9211-9216, Proceedings of the National Academy of Sciences of the United States of America
— id: 45996, year: 2004, vol: 101, page: 9211, stat: Journal Article,

Structural studies of HIV-1 Rev
Mayas, JF; Belasco, JG
2004 AUG ;13(3):134-134, Protein science
— id: 55683, year: 2004, vol: 13, page: 134, stat: Journal Article,

(Correction of Previews 200200622929. Two distinct regions on the surface of an RNA-binding domain are crucial for RNase E function. Correction of abstract.)
Diwa, Alexis A.; Jiang, Xunqing; Schapira, Matthieu; Belasco, Joel G.
2003 ;47(4):9005-9012 February, Molecular microbiology
— id: 98804, year: 2003, vol: 47, page: 9005, stat: Journal Article,

Critical features of a conserved RNA stem-loop important for feedback regulation of RNase E synthesis
Diwa, Alexis A; Belasco, Joel G
2002 Jun 7;277(23):20415-20422, Journal of biological chemistry
— id: 39691, year: 2002, vol: 277, page: 20415, stat: Journal Article,

Two distinct regions on the surface of an RNA-binding domain are crucial for RNase E function
Diwa, Alexis A; Jiang, Xunqing; Schapira, Matthieu; Belasco, Joel G
2002 Nov;46(4):959-969, Molecular microbiology
— id: 39374, year: 2002, vol: 46, page: 959, stat: Journal Article,

Comparative analysis of the plant mRNA-destabilizing element, DST, in mammalian and tobacco cells
Feldbrugge, M; Arizti, P; Sullivan, M L; Zamore, P D; Belasco, J G; Green, P J
2002 May;49(2):215-223, Plant molecular biology
— id: 106566, year: 2002, vol: 49, page: 215, stat: Journal Article,

Consequences of RNase E scarcity in Escherichia coli
Jain, Chaitanya; Deana, Atilio; Belasco, Joel G
2002 Feb;43(4):1053-1064, Molecular microbiology
— id: 39686, year: 2002, vol: 43, page: 1053, stat: Journal Article,

T7 phage display: a novel genetic selection system for cloning RNA-binding proteins from cDNA libraries
Danner S; Belasco JG
2001 Nov 6;98(23):12954-12959, Proceedings of the National Academy of Sciences of the United States of America
— id: 26570, year: 2001, vol: 98, page: 12954, stat: Journal Article,

Structural Model for the Cooperative Assembly of HIV-1 Rev Multimers on the RRE as Deduced from Analysis of Assembly-Defective Mutants
Jain C; Belasco JG
2001 Mar 1;7(3):603-614, Molecular cell
— id: 21108, year: 2001, vol: 7, page: 603, stat: Journal Article,

An evolutionarily conserved RNA stem-loop functions as a sensor that directs feedback regulation of RNase E gene expression
Diwa A; Bricker AL; Jain C; Belasco JG
2000 May 15;14(10):1249-1260, Genes & development
— id: 11692, year: 2000, vol: 14, page: 1249, stat: Journal Article,

Rapid genetic analysis of RNA-protein interactions by translational repression in Escherichia coli [In Process Citation]
Jain C; Belasco JG
2000 ;318:309-332, Methods in enzymology
— id: 11605, year: 2000, vol: 318, page: 309, stat: Journal Article,

Regions of RNase E important for 5'-end-dependent RNA cleavage and autoregulated synthesis
Jiang X; Diwa A; Belasco JG
2000 May;182(9):2468-2475, Journal of bacteriology
— id: 11758, year: 2000, vol: 182, page: 2468, stat: Journal Article,

Importance of a 5' stem-loop for longevity of papA mRNA in Escherichia coli
Bricker AL; Belasco JG
1999 Jun;181(11):3587-3590, Journal of bacteriology
— id: 14506, year: 1999, vol: 181, page: 3587, stat: Journal Article,

mRNA stabilization by the ompA 5' untranslated region: two protective elements hinder distinct pathways for mRNA degradation
Arnold TE; Yu J; Belasco JG
1998 Mar;4(3):319-330, RNA
— id: 57097, year: 1998, vol: 4, page: 319, stat: Journal Article,

RNA-binding proteins tamed
Laird-Offringa IA; Belasco JG
1998 Aug;5(8):665-668, Nature structural biology
— id: 14507, year: 1998, vol: 5, page: 665, stat: Journal Article,

Target discrimination by RNA-binding proteins: role of the ancillary protein U2A' and a critical leucine residue in differentiating the RNA-binding specificity of spliceosomal proteins U1A and U2B"
Rimmele ME; Belasco JG
1998 Nov;4(11):1386-1396, RNA
— id: 57067, year: 1998, vol: 4, page: 1386, stat: Journal Article,

RNA recognition by the joint action of two nucleolin RNA-binding domains: genetic analysis and structural modeling
Bouvet P; Jain C; Belasco JG; Amalric F; Erard M
1997 Sep 1;16(17):5235-5246, EMBO journal
— id: 14508, year: 1997, vol: 16, page: 5235, stat: Journal Article,

Translation of the adhE transcript to produce ethanol dehydrogenase requires RNase III cleavage in Escherichia coli
Aristarkhov A; Mikulskis A; Belasco JG; Lin EC
1996 Jul;178(14):4327-4332, Journal of bacteriology
— id: 14510, year: 1996, vol: 178, page: 4327, stat: Journal Article,

A structural model for the HIV-1 Rev-RRE complex deduced from altered-specificity rev variants isolated by a rapid genetic strategy
Jain C; Belasco JG
1996 Oct 4;87(1):115-125, Cell
— id: 14509, year: 1996, vol: 87, page: 115, stat: Journal Article,

In vitro genetic analysis of RNA-binding proteins using phage display libraries
Laird-Offringa IA; Belasco JG
1996 ;267:149-168, Methods in enzymology
— id: 14511, year: 1996, vol: 267, page: 149, stat: Journal Article,

Autoregulation of RNase E synthesis in Escherichia coli
Jain C; Belasco JG
1995 ;(33):85-88, Nucleic acids symposium series
— id: 14514, year: 1995, vol: , page: 85, stat: Journal Article,

RNase E autoregulates its synthesis by controlling the degradation rate of its own mRNA in Escherichia coli: unusual sensitivity of the rne transcript to RNase E activity
Jain C; Belasco JG
1995 Jan 1;9(1):84-96, Genes & development
— id: 14515, year: 1995, vol: 9, page: 84, stat: Journal Article,

Analysis of RNA-binding proteins by in vitro genetic selection: identification of an amino acid residue important for locking U1A onto its RNA target
Laird-Offringa IA; Belasco JG
1995 Dec 5;92(25):11859-11863, Proceedings of the National Academy of Sciences of the United States of America
— id: 14512, year: 1995, vol: 92, page: 11859, stat: Journal Article,

The nonamer UUAUUUAUU is the key AU-rich sequence motif that mediates mRNA degradation
Zubiaga AM; Belasco JG; Greenberg ME
1995 Apr;15(4):2219-2230, Molecular & cellular biology
— id: 14513, year: 1995, vol: 15, page: 2219, stat: Journal Article,

The ompA 5' untranslated region impedes a major pathway for mRNA degradation in Escherichia coli
Hansen MJ; Chen LH; Fejzo ML; Belasco JG
1994 Jun;12(5):707-716, Molecular microbiology
— id: 14516, year: 1994, vol: 12, page: 707, stat: Journal Article,

Multiple elements in the c-fos protein-coding region facilitate mRNA deadenylation and decay by a mechanism coupled to translation
Schiavi SC; Wellington CL; Shyu AB; Chen CY; Greenberg ME; Belasco JG
1994 Feb 4;269(5):3441-3448, Journal of biological chemistry
— id: 14517, year: 1994, vol: 269, page: 3441, stat: Journal Article,

Control of messenger RNA stability
Belasco, Joel G; Brawerman, George
San Diego CA : Academic Press, 1993,
— id: 2085, year: 1993, vol: , page: , stat: ,

The destabilizing elements in the coding region of c-fos mRNA are recognized as RNA
Wellington CL; Greenberg ME; Belasco JG
1993 Aug;13(8):5034-5042, Molecular & cellular biology
— id: 14518, year: 1993, vol: 13, page: 5034, stat: Journal Article,

Control of RNase E-mediated RNA degradation by 5'-terminal base pairing in E. coli
Bouvet P; Belasco JG
1992 Dec 3;360(6403):488-491, Nature
— id: 14520, year: 1992, vol: 360, page: 488, stat: Journal Article,

A 5'-terminal stem-loop structure can stabilize mRNA in Escherichia coli
Emory SA; Bouvet P; Belasco JG
1992 Jan;6(1):135-148, Genes & development
— id: 14521, year: 1992, vol: 6, page: 135, stat: Journal Article,

Regulation of proto-oncogene mRNA stability
Schiavi SC; Belasco JG; Greenberg ME
1992 Dec 16;1114(2-3):95-106, Biochimica & biophysica acta
— id: 14519, year: 1992, vol: 1114, page: 95, stat: Journal Article,

Autoregulation through translation
Wellington, C L; Belasco, J G
1992 Apr;2(4):216-218, Current biology. CB
— id: 106567, year: 1992, vol: 2, page: 216, stat: Journal Article,

Structure and function of a bacterial mRNA stabilizer: analysis of the 5' untranslated region of ompA mRNA
Chen LH; Emory SA; Bricker AL; Bouvet P; Belasco JG
1991 Aug;173(15):4578-4586, Journal of bacteriology
— id: 14522, year: 1991, vol: 173, page: 4578, stat: Journal Article,

Two distinct destabilizing elements in the c-fos message trigger deadenylation as a first step in rapid mRNA decay
Shyu AB; Belasco JG; Greenberg ME
1991 Feb;5(2):221-231, Genes & development
— id: 14523, year: 1991, vol: 5, page: 221, stat: Journal Article,

Degradation of pufLMX mRNA in Rhodobacter capsulatus is initiated by nonrandom endonucleolytic cleavage
Chen CY; Belasco JG
1990 Aug;172(8):4578-4586, Journal of bacteriology
— id: 14524, year: 1990, vol: 172, page: 4578, stat: Journal Article,

The ompA 5' untranslated RNA segment functions in Escherichia coli as a growth-rate-regulated mRNA stabilizer whose activity is unrelated to translational efficiency
Emory SA; Belasco JG
1990 Aug;172(8):4472-4481, Journal of bacteriology
— id: 14525, year: 1990, vol: 172, page: 4472, stat: Journal Article,

Deadenylylation: a mechanism controlling c-fos mRNA decay
Greenberg ME; Shyu AB; Belasco JG
1990 ;44(1-4):181-192, Enzyme
— id: 14526, year: 1990, vol: 44, page: 181, stat: Journal Article,

The c-fos transcript is targeted for rapid decay by two distinct mRNA degradation pathways
Shyu AB; Greenberg ME; Belasco JG
1989 Jan;3(1):60-72, Genes & development
— id: 14527, year: 1989, vol: 3, page: 60, stat: Journal Article,

Mechanism of puf mRNA degradation: the role of an intercistronic stem-loop structure
Belasco JG; Chen CY
1988 Dec 10;72(1-2):109-117, Gene
— id: 14528, year: 1988, vol: 72, page: 109, stat: Journal Article,

Mechanisms of mRNA decay in bacteria: a perspective
Belasco JG; Higgins CF
1988 Dec 10;72(1-2):15-23, Gene
— id: 14529, year: 1988, vol: 72, page: 15, stat: Journal Article,

An intercistronic stem-loop structure functions as an mRNA decay terminator necessary but insufficient for puf mRNA stability
Chen CY; Beatty JT; Cohen SN; Belasco JG
1988 Feb 26;52(4):609-619, Cell
— id: 14530, year: 1988, vol: 52, page: 609, stat: Journal Article,

Effect of premature termination of translation on mRNA stability depends on the site of ribosome release
Nilsson G; Belasco JG; Cohen SN; von Gabain A
1987 Jul;84(14):4890-4894, Proceedings of the National Academy of Sciences of the United States of America
— id: 14531, year: 1987, vol: 84, page: 4890, stat: Journal Article,

Energetics of proline racemase: double fractionation experiment, a test for concertedness and for transition-state dominance
Belasco JG; Albery WJ; Knowles JR
1986 May 6;25(9):2552-2558, Biochemistry
— id: 14535, year: 1986, vol: 25, page: 2552, stat: Journal Article,

Energetics of proline racemase: fractionation factors for the essential catalytic groups in the enzyme-substrate complexes
Belasco JG; Bruice TW; Albery WJ; Knowles JR
1986 May 6;25(9):2558-2564, Biochemistry
— id: 14534, year: 1986, vol: 25, page: 2558, stat: Journal Article,

Energetics of proline racemase: rates, fractionation factors, and buffer catalysis in the oversaturated region. Nature of the interconversion of the two forms of free enzyme
Belasco JG; Bruice TW; Fisher LM; Albery WJ; Knowles JR
1986 May 6;25(9):2564-2571, Biochemistry
— id: 14533, year: 1986, vol: 25, page: 2564, stat: Journal Article,

The stability of E. coli gene transcripts is dependent on determinants localized to specific mRNA segments
Belasco JG; Nilsson G; von Gabain A; Cohen SN
1986 Jul 18;46(2):245-251, Cell
— id: 14532, year: 1986, vol: 46, page: 245, stat: Journal Article,

Energetics of proline racemase: transition-state fractionation factors for the two protons involved in the catalytic steps
Fisher LM; Belasco JG; Bruice TW; Albery WJ; Knowles JR
1986 May 6;25(9):2543-2551, Biochemistry
— id: 14536, year: 1986, vol: 25, page: 2543, stat: Journal Article,

Differential expression of photosynthesis genes in R. capsulata results from segmental differences in stability within the polycistronic rxcA transcript
Belasco JG; Beatty JT; Adams CW; von Gabain A; Cohen SN
1985 Jan;40(1):171-181, Cell
— id: 14537, year: 1985, vol: 40, page: 171, stat: Journal Article,

Growth-rate dependent regulation of mRNA stability in Escherichia coli
Nilsson G; Belasco JG; Cohen SN; von Gabain A
1984 Nov 1-7;312(5989):75-77, Nature
— id: 14538, year: 1984, vol: 312, page: 75, stat: Journal Article,

Polarization of substrate carbonyl groups by yeast aldolase: investigation by Fourier transform infrared spectroscopy
Belasco JG; Knowles JR
1983 Jan 4;22(1):122-129, Biochemistry
— id: 14540, year: 1983, vol: 22, page: 122, stat: Journal Article,

Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts
von Gabain A; Belasco JG; Schottel JL; Chang AC; Cohen SN
1983 Feb;80(3):653-657, Proceedings of the National Academy of Sciences of the United States of America
— id: 14539, year: 1983, vol: 80, page: 653, stat: Journal Article,

Direct observation of substrate distortion by triosephosphate isomerase using Fourier transform infrared spectroscopy
Belasco JG; Knowles JR
1980 Feb 5;19(3):472-477, Biochemistry
— id: 14543, year: 1980, vol: 19, page: 472, stat: Journal Article,

Spectroscopic and kinetic probes of enzyme catalysis
Belasco, Joel G
[S.l. : s.n.], 1980,
— id: 2086, year: 1980, vol: , page: , stat: ,

Beta-lactamase inactivation by mechanism-based reagents
Fisher J; Belasco JG; Charnas RL; Khosla S; Knowles JR
1980 May 16;289(1036):309-319, Philosophical transactions of the Royal Society of London. Series B. Biological sciences
— id: 14542, year: 1980, vol: 289, page: 309, stat: Journal Article,

beta-Lactamase proceeds via an acyl-enzyme intermediate. Interaction of the Escherichia coli RTEM enzyme with cefoxitin
Fisher J; Belasco JG; Khosla S; Knowles JR
1980 Jun 24;19(13):2895-2901, Biochemistry
— id: 14541, year: 1980, vol: 19, page: 2895, stat: Journal Article,

Critical ionization states in the reaction catalyzed by triosephosphate isomerase
Belasco JG; Herlihy JM; Knowles JR
1978 Jul 25;17(15):2971-2978, Biochemistry
— id: 14544, year: 1978, vol: 17, page: 2971, stat: Journal Article,