Bioinformatics Syllabus
Bioinformatics
Spring 2012
Instructor: Dr. Stuart Brown,
Associate Professor, NYUMC Center for Health Informatics & Bioinformatics
Schedule
Lectures/Lab: Tuesesdays, Jan 24-May 1, 1-4:30 PM,
Location - NYUMC Center for Bioinformatics: Verizon Bldg, 227 E 30th St., 7th fl conf room.
Textbooks:
Understanding Bioinformatics
by Marketa Zvelebil and Jeremy Baum
Beginning Perl for Bioinformatics
by James Tisdall
Also Recommended:
Bioinformatics, A Practical Guide to the Analysis of Genes and Proteins
by A.D. Baxevanis and B.F. Ouellettee
(free online for NYU students: http://www3.interscience.wiley.com/cgi-bin/booktoc/104086862)
Bioinformatics: Sequence and Genome Analysis
by David W. Mount
Essentials of Medical Genomics
by Stuart M. Brown
Bioinformatics for Dummies
by Jean-Michel Claverie & Cedric Notredame
Blast
by Ian Korf, Mark Yandell, and Joseph Bedell
This is a practical course in Bioinformatics which will emphasize how to use the computer as a tool for biomedical research. Prerequsites include a thorough understanding of theoretical and practical aspects of molecular biology, and some University level mathematics and statistics, but no prior knowledge of computer programming or computer hardware is necessary.
Spring 2012 Course Syllabus (G16.2604):
[NOTE: Lectures & exercises posted here are subject to change up to minutes before they are actually presented in class]
Lecture 1. Introduction to the Course: Bionformatics Algorithms & Data Structures
Tues, Jan 24
Lecture 1 PowerPoint slides
Exercise for Lecture 1
- Overview of the challenges of Bioinformatics in the era of Genomics
Lecture 2. Bioinformatics Databases, UNIX Basics (part 1)
Tues, Jan 30
Reading
Why Biologists Want to Program Computers by James Tisdall
A Simple video on the use of Entrez/PubMed
Entrez Tutorial
Unix Exercise
Lecture 3. Alignment, UNIX Basics (part 2)
Tues, Feb 7
Alignment PowerPoint slides
Unix II PowerPoint slides
Unix text editing exercise for Lecture 3
Alignment exercise
Reading
How Perl Saved the Human Genome Project by Lincoln Stein
Emacs Reference Guide
Lecture 4. Similarity Searching (BLAST), Perl (part 1)
Tues, Feb 14
Lecture 4 PowerPoint slides
Perl 2 lecture ppt slides
Perl-1 PowerPoint
Perl Exercise 1
Searching Databases by Similarity
O'Reilly BLAST book chap 4.
Similarity Searching on the Web
Fun BLAST exercise
Optional addtional exercise on BLAST from NCBI
Exercise 1 for Lecture 4
Lecture 5. Advanced Alignment methods, Perl (part 2)
Tues, Feb 21
Reading:
Advanced Similarity Searching on the Web
Searching Sequence Databases A novel Src kinase in the C.elegans genome
Lecture 6. Patterns and Sequence-Function Relationships, Perl (part 3)
Tues, Feb 28
Lecture 6 PowerPoint slides
Pattern Exercise
Web motif & function Exercise
Protein structure tutorial with Cn3D
Perl3 RegExpr PPT
Perl Exercise #3 will be graded for a Midterm.
Lecture 7. Multiple Alignment,
Tues, March 6
Reading:
Multiple Alignment on the Web
Exercises
Multiple alignment exercise
[Extra week to work on Perl Ex #3]
Lecture 8. Computing Evolution: Phylogenetic Analysis
Tues, March 13
Phylogenetics PowerPoint slides
Comparative Genomics
Phylogenetics Exercise
Marine Mammals phylogenetics exercise (Fun!)
Reading
Ks/Ka score detects evolution
- Sequence-based taxonomy: Overview and Assumptions
- From Multiple Alignment to Phylogeny
- Detecting selection in sequences
INSERT SOME SORT OF more objective MIDTERM EXAM HERE
Lecture 9. Genomics I: SNPs and Haplotypes
Tues, March 20
Readings
Exercise
Lecture 10. Genomics II: Analysis of Microarray Data
Tues, March 27
Microarray PowerPoint slides
Exrcises Array Express Tutorial
Microarray Exercise
Reading:
Gene Expression Data Analysis
- Basics of designing a microarray
- Image analysis
- Normalization
- Variability and replication
- Clustering
- Microarray Databases
Lecture 11. Genomics III: Next-Geration Sequencing (part 1): Sequence Alignment & Variation
Tues, April 3
- New Sequencing Technologies
- Genome assembly
- Mutation discovery
-
- Lecture 12, Genomics IV: Next-Geration Sequencing (part 2): ChIP-seq & RNA-seq
- Tues, April 10
- Tues April 17:
Spillover if we need to more time on any topics, discusssion of work in progress on projects,
- Option of an additional lecture on Microbial sequencing, assembly & Metagenomics
- Tues May 1 (or TDB)
Poster/project presentations
Examples of Posters for course projects