Sequence Range: 1 to 4363

Enzyme      	#Cuts	Positions

AatII       	    1	  4290	
AflIII      	    1	  2475	
AhdI        	    1	  3368	
AlwNI       	    1	  2891	
AseI        	    1	  3540	
AvaI        	    1	  1425	
BamHI       	    1	   375	
BsaI        	    1	  3429	
BsaAI       	    1	  2229	
BsaBI       	    1	  1672	
BsgI        	    1	  1635	
BsmI        	    1	  1359	
BsmBI       	    1	  2118	
BsoBI       	    1	  1425	
BspDI       	    1	    24	
BspEI       	    1	  1664	
BspMI       	    1	  1054	
Bst1107I    	    1	  2248	
ClaI        	    1	    24	
EagI        	    1	   939	
EcoNI       	    1	   626	
EcoRI       	    1	  4361	
EcoRV       	    1	   187	
HindIII     	    1	    29	
MscI        	    1	  1446	
NdeI        	    1	  2298	
NheI        	    1	   229	
NruI        	    1	   974	
PstI        	    1	  3613	
PvuI        	    1	  3738	
PvuII       	    1	  2068	
SalI        	    1	   651	
SapI        	    1	  2359	
ScaI        	    1	  3848	
SphI        	    1	   566	
SspI        	    1	  4172	
StyI        	    1	  1369	
Tth111I     	    1	  2222