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The availability of genetic analysis and transgenic techniques in the mouse have led to its widespread acceptance as the preferred animal model for studying mammalian embryonic development and many human diseases. Ultrasound and magnetic resonance (MR) micro-imaging methods are being developed in the Turnbull laboratory for noninvasive analysis of development and disease in genetically-engineered mouse models.
A 40-50 MHz ultrasound biomicroscope (UBM) has been developed for imaging and measuring hemodynamic parameters in utero through all the critical stages of early brain and heart development, enabling dynamic analysis of mutant and transgenic mice with brain and heart defects. For direct manipulation of mouse embryos, a system for in utero UBM-guided injection of cells and viruses was developed, which is currently being used to study cell lineage and fate in the mouse embryonic brain, and to induce ectopic gene expression using retroviruses.
A number of in vivo MR micro-imaging imaging methods have been developed on a 7-Tesla MRI to study brain tumor development and progressive neuro-degeneration in genetically-engineered mouse models of Multiple Sclerosis and Alzheimer?s disease. Contrast-enhancement with manganese is being investigated for imaging early postnatal brain development in the mouse, and for mapping neural activity in the adult and developing brain. Finally, targeted contrast-labeling approaches are being developed and tested to detect and monitor disease processes and for cellular imaging in the developing and diseased mouse brain.
Multimodal Genetic Approach for Molecular Imaging of Vasculature in a Mouse Model of Melanoma
Suero-Abreu, Giselle A; Aristizabal, Orlando; Bartelle, Benjamin B; Volkova, Eugenia; Rodriguez, Joe J; Turnbull, Daniel H. Multimodal Genetic Approach for Molecular Imaging of Vasculature in a Mouse Model of Melanoma. Molecular imaging & biology. 2016 Sep 27;:?-? (2262552)
A Mathematical Model of Granule Cell Generation During Mouse Cerebellum Development
Leffler, Shoshana R; Legue, Emilie; Aristizabal, Orlando; Joyner, Alexandra L; Peskin, Charles S; Turnbull, Daniel H. A Mathematical Model of Granule Cell Generation During Mouse Cerebellum Development. Bulletin of mathematical biology. 2016 May;78(5):859-878 (2092612)
Cardiovascular Imaging in Mice
Phoon, Colin K L; Turnbull, Daniel H. Cardiovascular Imaging in Mice. Current protocols in mouse biology. 2016 Mar 01;6(1):15-38 (2006292)
Engineering an effective Mn-binding MRI reporter protein by subcellular targeting
Bartelle, Benjamin B; Mana, Miyeko D; Suero-Abreu, Giselle A; Rodriguez, Joe J; Turnbull, Daniel H. Engineering an effective Mn-binding MRI reporter protein by subcellular targeting. Magnetic resonance in medicine. 2015 Dec;74(6):1750-1757 (1411432)
4D MEMRI atlas of neonatal FVB/N mouse brain development
Szulc, Kamila U; Lerch, Jason P; Nieman, Brian J; Bartelle, Benjamin B; Friedel, Miriam; Suero-Abreu, Giselle A; Watson, Charles; Joyner, Alexandra L; Turnbull, Daniel H. 4D MEMRI atlas of neonatal FVB/N mouse brain development. Neuroimage. 2015 Sep;118:49-62 (1615482)