Published on NYU Langone Medical Center (http://www.med.nyu.edu)

Home > Printer-friendly > Printer-friendly

Printer-friendly

Printer-friendly

Biosketch / Results /

Alan B Frey, Ph.D.

Associate Professor; Dir Instructor Lecture Immunology
Department of Cell Biology (Cell Biology) [1]

Contact Info

Address
550 First Avenue
Floor 9 Room 907
Smilow Research Building
New York, NY 10016

212-263-8129, 212-263-5354
212-263-8139
Alan.Frey@nyumc.org

[2]

« Back to Results [6]

Education

— Dr. Frey was a Princeton University Postdoctoral Fellow in Molecular Biology, PostDoctoral Training
— Sackler Institute of Biological Sciences, Medical Education
1985 — New York University, Graduate Education

« Back to Results [6]

Research Summary

Cancers are antigenic and recruit CD8+ T cells into the tumor tissue, referred to as ''TIL'' for tumor infiltrating lymphocytes. However, antitumor T cells are dysfunctional and are defective in cytolysis. Since exocytosis of lytic granules from T cells may potentially be dependent upon Immune Synapse (IS) formation and non-lytic tumor infiltrating lymphocytes (TIL) cannot exocytose granules, we considered that TIL IS formation or function is defective. Therefore, in conjugates formed between TIL and cognate tumor cells, using a combination of confocal microscopy and biochemical assays, we investigated the localization and activation status of proteins important in signal transduction, IS formation, and lytic function. Signal transduction in freshly-isolated, non-lytic TIL is defective: they do not flux calcium, activate PLCI?-1, increase protein tyrosine phosphorylation, or recruit WASp, Pyk-2, F-actin and the microtubule organizing center (MTOC) to the target contact site (CS) formed between TIL and cognate target cells. The block in signaling is proximal since LAT is not phosphorylated and ZAP70, although recruited to the CS, is only weakly activated. Importantly, the inhibitory motif in p56lck (Y505) becomes rapidly phosphorylated upon binding to cognate tumor cells. Consistent with a proximal signaling defect, Csk is recruited to the plasma membrane and Shp-2 is retained in the cytoplasm. In addition, Shp-1 localizes at the CS where it may mediate de-activation of various SH3-containing proteins (such as ZAP70) therein preventing propagation of the activation signal. Furthermore, we showed that upon contact with cognate target cells, non-lytic TIL assemble many signaling components (TCRI?I?, CD3I?, p56lck, ZAP70, LFA-1, LAT, and lipid rafts) with kinetics typical of activated CD8+ T cells, showing that non-lytic TIL are triggered by conjugate formation. However, CD2, the CD3 complex, and CD8, which associate with the TCR prior to conjugate formation, rapidly dissociate and are excluded from the CS. Tumor-induced disruption of T cell activation at a point downstream of triggering, therein blocking proximal tyrosine kinase activity, calcium flux, and dependent lytic function, is a novel mechanism for inhibition of the CD8+ T cell effector phase. Collectively our data suggests that proximal TCR-mediated signaling in non-lytic TIL is rapidly blocked after conjugation with cognate tumor target cells. The signaling defect likely is mediated by tumor-induced enhanced Shp-1 activity in TIL which prevents activation of proximal tyrosine kinase activity, affinity upregulation of LFA-1, and calcium flux ultimately preventing exocytosis of lytic granules and lysis of tumor cells. Importantly, the TIL lytic defect is an acquired property restricted to T cells within the tumor microenvironment since in vivo systemic T cell function is not affected by tumor growth whereas TIL are characterized by the inability to mobilize the MTOC to the CS and exocytose lytic granules. Acquired transient lytic defects in TIL have also been described in several transgenic TCR mouse tumor models. Consideration of the observation that human TIL are antigen-specific but non-lytic, together with our description of defective lytic function of murine TIL, supports the notion that tumor-induced inhibition of TIL lytic function may be a common characteristic which may contribute to tumor growth in the presence of antitumor immune response. Tumor-induced lytic dysfunction also may restrict T cell based immunotherapy of cancer. Current research investigates the detailed biochemical basis of the tumor-induced profound defect in T cell signaling.

Research Interests

CD8+ T Cell immune response in cancer

Research Keywords

immune response in cancer, tumor immunology, vaccine therapy<br><br>

Research Documents

tumor-induced TIL signaling defect [7]
[8]

« Back to Results [6]

All data from NYU Health Sciences Library Faculty Bibliography — -

Contact:
http://hsl.med.nyu.edu/faculty-bibliography-search [9]

A novel p56lck-interacting protein expressed in CD8+memory cells and tumor infiltrating T cells that inhibits proximal TCR-mediated signaling
Frey, Alan; Burns, Jeremy; Vazquez-Cintron, Edwin
2012 MAY 1;188:-, Journal of immunology
— id: 169546, year: 2012, vol: 188, page: , stat: Journal Article,

Dendritic Cell Populations With Different Concentrations of Lipid Regulate Tolerance and Immunity in Mouse and Human Liver
Ibrahim, J; Nguyen, AH; Rehman, A; Ochi, A; Jamal, M; Graffeo, CS; Henning, JR; Zambirinis, CP; Fallon, N; Barilla, R; Badar, S; Mitchell, A; Rao, R; Acehan, D; Frey, AB; Miller, G
2012 Jun;:1061-1072, Gastroenterology
— id: 172916, year: 2012, vol: , page: 1061, stat: Journal Article,

Effect of clazosentan on clinical outcome after aneurysmal subarachnoid hemorrhage and endovascular coiling: Results of the conscious-3 study
Macdonald, R L; Higashida, R; Keller, E; Mayer, S A; Molyneux, A J; Raabe, A; Vajkoczy, P; Wanke, I; Bach, D; Frey, A; Nowbakht, P; Roux, S; Kassell, N F
2012 February, 2012;43(2):-, Stroke
— id: 185372, year: 2012, vol: 43, page: , stat: Journal Article,

Myeloid-Derived Suppressor Cells and anti-tumor T cells: a complex relationship
Monu, Ngozi R; Frey, Alan B
2012 ;41(6-7):595-613, Immunological investigations
— id: 179092, year: 2012, vol: 41, page: 595, stat: Journal Article,

MyD88 inhibition amplifies dendritic cell capacity to promote pancreatic carcinogenesis via Th2 cells
Ochi, Atsuo; Nguyen, Andrew H; Bedrosian, Andrea S; Mushlin, Harry M; Zarbakhsh, Saman; Barilla, Rocky; Zambirinis, Constantinos P; Fallon, Nina C; Rehman, Adeel; Pylayeva-Gupta, Yuliya; Badar, Sana; Hajdu, Cristina H; Frey, Alan B; Bar-Sagi, Dafna; Miller, George
2012 Aug;209(9):1671-1687, Journal of experimental medicine
— id: 177029, year: 2012, vol: 209, page: 1671, stat: Journal Article,

Protocadherin-18 is a novel differentiation marker and an inhibitory signaling receptor for CD8(+) effector memory T cells
Vazquez-Cintron, Edwin J; Monu, Ngozi R; Burns, Jeremy C; Blum, Roy; Chen, Gregory; Lopez, Peter; Ma, Jennifer; Radoja, Sasa; Frey, Alan B
2012 ;7(5):e36101-e36101 e36101, PLoS ONE
— id: 166801, year: 2012, vol: 7, page: e36101, stat: Journal Article,

Neuropilin 1 is expressed on thymus-derived natural regulatory T cells, but not mucosa-generated induced Foxp3+ T reg cells
Weiss, Jonathan M; Bilate, Angelina M; Gobert, Michael; Ding, Yi; Curotto de Lafaille, Maria A; Parkhurst, Christopher N; Xiong, Huizhong; Dolpady, Jayashree; Frey, Alan B; Ruocco, Maria Grazia; Yang, Yi; Floess, Stefan; Huehn, Jochen; Oh, Soyoung; Li, Ming O; Niec, Rachel E; Rudensky, Alexander Y; Dustin, Michael L; Littman, Dan R; Lafaille, Juan J
2012 Sep;209(10):2291-2299, Journal of experimental medicine
— id: 178843, year: 2012, vol: 209, page: 2291, stat: Journal Article,

Dendritic cells promote pancreatic viability in mice with acute pancreatitis
Bedrosian, Andrea S; Nguyen, Andrew H; Hackman, Michael; Connolly, Michael K; Malhotra, Ashim; Ibrahim, Junaid; Cieza-Rubio, Napoleon E; Henning, Justin R; Barilla, Rocky; Rehman, Adeel; Pachter, H Leon; Medina-Zea, Marco V; Cohen, Steven M; Frey, Alan B; Acehan, Devrim; Miller, George
2011 Nov;141(5):1915-26.e1, Gastroenterology
— id: 139730, year: 2011, vol: 141, page: 1915, stat: Journal Article,

In hepatic fibrosis, liver sinusoidal endothelial cells acquire enhanced immunogenicity
Connolly, Michael K; Bedrosian, Andrea S; Malhotra, Ashim; Henning, Justin R; Ibrahim, Junaid; Vera, Valery; Cieza-Rubio, Napoleon E; Hassan, Burhan U; Pachter, H Leon; Cohen, Steven; Frey, Alan B; Miller, George
2010 Aug 15;185(4):2200-2208, Journal of immunology
— id: 111819, year: 2010, vol: 185, page: 2200, stat: Journal Article,

Distinct populations of metastases-enabling myeloid cells expand in the liver of mice harboring invasive and preinvasive intra-abdominal tumor
Connolly, Michael K; Mallen-St Clair, Jon; Bedrosian, Andrea S; Malhotra, Ashim; Vera, Valery; Ibrahim, Junaid; Henning, Justin; Pachter, H Leon; Bar-Sagi, Dafna; Frey, Alan B; Miller, George
2010 Apr;87(4):713-725, Journal of leukocyte biology
— id: 108918, year: 2010, vol: 87, page: 713, stat: Journal Article,

Tumor-induced disruption of proximal TCR-mediated signal transduction in tumor-infiltrating CD8+ lymphocytes inactivates antitumor effector phase
Vazquez-Cintron, Edwin J; Monu, Ngozi R; Frey, Alan B
2010 Dec 15;185(12):7133-7140, Journal of immunology
— id: 116210, year: 2010, vol: 185, page: 7133, stat: Journal Article,

In liver fibrosis, dendritic cells govern hepatic inflammation in mice via TNF-alpha
Connolly, Michael K; Bedrosian, Andrea S; Mallen-St Clair, Jon; Mitchell, Aaron P; Ibrahim, Junaid; Stroud, Andrea; Pachter, H Leon; Bar-Sagi, Dafna; Frey, Alan B; Miller, George
2009 Nov;119(11):3213-3225, Journal of clinical investigation
— id: 105172, year: 2009, vol: 119, page: 3213, stat: Journal Article,

Signaling defects in anti-tumor T cells
Frey, Alan B; Monu, Ngozi
2008 Apr;222:192-205, Immunological reviews
— id: 92674, year: 2008, vol: 222, page: 192, stat: Journal Article,

Skewing the Th cell phenotype toward Th1 alters the maturation of tumor-infiltrating mononuclear phagocytes
Nonaka, Kenichi; Saio, Masanao; Suwa, Tatsuhiko; Frey, Alan B; Umemura, Naoki; Imai, Hisashi; Ouyang, Guan-Feng; Osada, Shinji; Balazs, Margit; Adany, Roza; Kawaguchi, Yoshihiro; Yoshida, Kazuhiro; Takami, Tsuyoshi
2008 Sep;84(3):679-688, Journal of leukocyte biology
— id: 96103, year: 2008, vol: 84, page: 679, stat: Journal Article,

Osteopontin is linked to p65 and MMP-9 expression in pulmonary adenocarcinoma but not in malignant pleural mesothelioma
Frey, A B; Wali, A; Pass, H; Lonardo, F
2007 May;50(6):720-726, Histopathology
— id: 110900, year: 2007, vol: 50, page: 720, stat: Journal Article,

Protein kinase Cdelta regulates antigen receptor-induced lytic granule polarization in mouse CD8+ CTL
Ma, Jennifer S Y; Monu, Ngozi; Shen, David T; Mecklenbrauker, Ingrid; Radoja, Nadezda; Haydar, Tarik F; Leitges, Michael; Frey, Alan B; Vukmanovic, Stanislav; Radoja, Sasa
2007 Jun 15;178(12):7814-7821, Journal of immunology
— id: 96104, year: 2007, vol: 178, page: 7814, stat: Journal Article,

Suppression of proximal T cell receptor signaling and lytic function in CD8+ tumor-infiltrating T cells
Monu, Ngozi; Frey, Alan B
2007 Dec 1;67(23):11447-11454, Cancer research
— id: 75676, year: 2007, vol: 67, page: 11447, stat: Journal Article,

TNF expressed by tumor-associated macrophages, but not microglia, can eliminate glioma
Nakagawa, Jiro; Saio, Masanao; Tamakawa, Noriyuki; Suwa, Tatsuhiko; Frey, Alan B; Nonaka, Kenichi; Umemura, Naoki; Imai, Hisashi; Ouyang, Guan-Feng; Ohe, Naoyuki; Yano, Hirohito; Yoshimura, Sinichi; Iwama, Toru; Takami, Tsuyoshi
2007 Apr;30(4):803-811, International journal of oncology
— id: 96105, year: 2007, vol: 30, page: 803, stat: Journal Article,

Myeloid suppressor cells regulate the adaptive immune response to cancer
Frey, Alan B
2006 Oct;116(10):2587-2590, Journal of clinical investigation
— id: 69693, year: 2006, vol: 116, page: 2587, stat: Journal Article,

Effector-phase tolerance: another mechanism of how cancer escapes antitumor immune response
Frey, Alan B; Monu, Ngozi
2006 Apr;79(4):652-662, Journal of leukocyte biology
— id: 66694, year: 2006, vol: 79, page: 652, stat: Journal Article,

Defective adhesion in tumor infiltrating CD8+ T cells
Koneru, Mythili; Monu, Ngozi; Schaer, David; Barletta, Justine; Frey, Alan B
2006 May 15;176(10):6103-6111, Journal of immunology
— id: 64588, year: 2006, vol: 176, page: 6103, stat: Journal Article,

T-cell receptor signaling events triggering granule exocytosis
Radoja, Sasa; Frey, Alan B; Vukmanovic, Stanislav
2006 ;26(3):265-290, Critical reviews in immunology
— id: 67541, year: 2006, vol: 26, page: 265, stat: Journal Article,

Defective proximal TCR signaling inhibits CD8+ tumor-infiltrating lymphocyte lytic function
Koneru, Mythili; Schaer, David; Monu, Ngozi; Ayala, Aidee; Frey, Alan B
2005 Feb 15;174(4):1830-1840, Journal of immunology
— id: 50292, year: 2005, vol: 174, page: 1830, stat: Journal Article,

Immunocytochemical demonstration of down regulation of HLA class-I molecule expression in human metastatic breast carcinoma
Saio, Masanao; Teicher, Matt; Campbell, Gaynor; Feiner, Helen; Delgado, Yara; Frey, Alan B
2004 ;21(3):243-249, Clinical & experimental metastasis
— id: 67542, year: 2004, vol: 21, page: 243, stat: Journal Article,

Expression of rat complement control protein Crry on tumor cells inhibits rat natural killer cell-mediated cytotoxicity
Caragine, Theresa A; Imai, Masaki; Frey, Alan B; Tomlinson, Stephen
2002 Nov 1;100(9):3304-3310, Blood
— id: 93833, year: 2002, vol: 100, page: 3304, stat: Journal Article,

A tumor-expressed inhibitor of the early but not late complement lytic pathway enhances tumor growth in a rat model of human breast cancer
Caragine, Theresa A; Okada, Noriko; Frey, Alan B; Tomlinson, Stephen
2002 Feb 15;62(4):1110-1115, Cancer research
— id: 91293, year: 2002, vol: 62, page: 1110, stat: Journal Article,

The role of fibroblasts in thymocyte-positive selection
Lilic, Mirjana; Santori, Fabio R; Neilson, Eric G; Frey, Alan B; Vukmanovic, Stanislav
2002 Nov 1;169(9):4945-4950, Journal of immunology
— id: 34693, year: 2002, vol: 169, page: 4945, stat: Journal Article,

CD8(+) tumor-infiltrating lymphocytes are primed for Fas-mediated activation-induced cell death but are not apoptotic in situ
Radoja S; Saio M; Frey AB
2001 May 15;166(10):6074-6083, Journal of immunology
— id: 20291, year: 2001, vol: 166, page: 6074, stat: Journal Article,

CD8(+) tumor-infiltrating T cells are deficient in perforin-mediated cytolytic activity due to defective microtubule-organizing center mobilization and lytic granule exocytosis
Radoja S; Saio M; Schaer D; Koneru M; Vukmanovic S; Frey AB
2001 Nov 1;167(9):5042-5051, Journal of immunology
— id: 25552, year: 2001, vol: 167, page: 5042, stat: Journal Article,

Tumor-infiltrating macrophages induce apoptosis in activated CD8(+) T cells by a mechanism requiring cell contact and mediated by both the cell-associated form of TNF and nitric oxide
Saio M; Radoja S; Marino M; Frey AB
2001 Nov 15;167(10):5583-5593, Journal of immunology
— id: 25551, year: 2001, vol: 167, page: 5583, stat: Journal Article,

Protection against tick-transmitted Lyme disease in dogs vaccinated with a multiantigenic vaccine
Straubinger, RK; Rao, TD; Davidson, E; Summers, BA; Jacobson, RH; Frey, AB
2001 Oct 12;20(1-2):181-193, Vaccine
— id: 28209, year: 2001, vol: 20, page: 181, stat: Journal Article,

Type I IFN modulates innate and specific antiviral immunity
Durbin JE; Fernandez-Sesma A; Lee CK; Rao TD; Frey AB; Moran TM; Vukmanovic S; Garcia-Sastre A; Levy DE
2000 Apr 15;164(8):4220-4228, Journal of immunology
— id: 11766, year: 2000, vol: 164, page: 4220, stat: Journal Article,

Distinct requirements for IFNs and STAT1 in NK cell function
Lee CK; Rao DT; Gertner R; Gimeno R; Frey AB; Levy DE
2000 Oct 1;165(7):3571-3577, Journal of immunology
— id: 20292, year: 2000, vol: 165, page: 3571, stat: Journal Article,

Cancer-induced defective cytotoxic T lymphocyte effector function: another mechanism how antigenic tumors escape immune-mediated killing [In Process Citation]
Radoja S; Frey AB
2000 Jun;6(6):465-479, Molecular medicine
— id: 11514, year: 2000, vol: 6, page: 465, stat: Journal Article,

Mice bearing late-stage tumors have normal functional systemic T cell responses in vitro and in vivo
Radoja S; Rao TD; Hillman D; Frey AB
2000 Mar 1;164(5):2619-2628, Journal of immunology
— id: 8539, year: 2000, vol: 164, page: 2619, stat: Journal Article,

NKT cell cytokine imbalance in murine diabetes mellitus
Frey AB; Rao TD
1999 ;29(3):201-214, Autoimmunity
— id: 6171, year: 1999, vol: 29, page: 201, stat: Journal Article,

Protection from Lyme neuroborreliosis in nonhuman primates with a multiantigenic vaccine
Pachner AR; Delaney E; Zhang WF; O'Neill T; Major E; Frey AB; Davidson E
1999 Jun;91(3):310-313, Clinical immunology
— id: 8508, year: 1999, vol: 91, page: 310, stat: Journal Article,

Multidose streptozotocin induction of diabetes in BALB/cBy mice induces a T cell proliferation defect in thymocytes which is reversible by interleukin-4
Wood SC; Rao TD; Frey AB
1999 Feb 25;192(1):1-12, Cellular immunology
— id: 6056, year: 1999, vol: 192, page: 1, stat: Journal Article,

Protection of human breast cancer cells from complement-mediated lysis by expression of heterologous CD59
Yu J; Caragine T; Chen S; Morgan BP; Frey AB; Tomlinson S
1999 Jan;115(1):13-18, Clinical & experimental immunology
— id: 7468, year: 1999, vol: 115, page: 13, stat: Journal Article,

Oncogenes and immunity: immune responses against products of transforming genes and the effects of oncogene activity on immunogenicity
Appleman L; Frey AB
Molecular approaches to tumor immunotherapy Singapore : World Scientific, 1998,
— id: 3588, year: 1998, vol: , page: ?, stat: Chapter,

Rat NKR-P1+ CD3+ T cells: selective proliferation in interleukin-2, diverse T-cell-receptor-Vbeta repertoire and polarized interferon-gamma expression
Badovinac V; Boggiano C; Trajkovic V; Frey AB; Vujanovic NL; Gold DP; Mostarica-Stojkovic M; Vukmanovic S
1998 Sep;95(1):117-125, Immunology
— id: 7999, year: 1998, vol: 95, page: 117, stat: Journal Article,

Repression of interleukin-2 mRNA translation in primary human breast carcinoma tumor-infiltrating lymphocytes
Lopez CB; Rao TD; Feiner H; Shapiro R; Marks JR; Frey AB
1998 Dec 15;190(2):141-155, Cellular immunology
— id: 7394, year: 1998, vol: 190, page: 141, stat: Journal Article,

Administration of silica sensitizes lipopolysaccharide responsiveness of murine macrophages but inhibits T and B cell priming by inhibition of antigen presenting function
Rao TD; Frey AB
1998 May;27(3):181-199, Immunological investigations
— id: 7751, year: 1998, vol: 27, page: 181, stat: Journal Article,

Soluble proteins isolated from Borrelia burgdorferi by extraction with Triton X-114 confer resistance to experimental infection
Rao TD; Frey AB
1998 Oct;89(1):94-104, Clinical immunology & immunopathology
— id: 7431, year: 1998, vol: 89, page: 94, stat: Journal Article,

Study of immune response to tumors in the rat
Frey AB
1997 Jun;12(2):173-188, Methods
— id: 7149, year: 1997, vol: 12, page: 173, stat: Journal Article,

Killing of rat adenocarcinoma 13762 in situ by adoptive transfer of CD4+ anti-tumor T cells requires tumor expression of cell surface MHC class II molecules
Frey AB; Cestari S
1997 May 25;178(1):79-90, Cellular immunology
— id: 7150, year: 1997, vol: 178, page: 79, stat: Journal Article,

Tumors antigens encoded by oncogenes and the impact of oncogenes upon the immune responses
Appleman LJ; Frey AB
1996 May 25;170(1):1-10, Cellular immunology
— id: 8048, year: 1996, vol: 170, page: 1, stat: Journal Article,

Role of host antigen receptor-bearing and antigen receptor-negative cells in immune response to rat adenocarcinoma 13762
Frey AB
1996 May 15;156(10):3841-3849, Journal of immunology
— id: 6995, year: 1996, vol: 156, page: 3841, stat: Journal Article,

Dominant-negative p53 can restore tumorigenicity of L-929 cells in immunocompetent mice
Appleman LJ; Frey AB
1995 Nov 15;63(4):576-583, International journal of cancer
— id: 7930, year: 1995, vol: 63, page: 576, stat: Journal Article,

Mouse embryo fibroblasts transformed by activated ras or dominant-negative p53 express cross-reactive tumor rejection antigens
Appleman LJ; Uyeki J; Frey AB
1995 Jun 9;61(6):887-894, International journal of cancer
— id: 12762, year: 1995, vol: 61, page: 887, stat: Journal Article,

Rat mammary adenocarcinoma 13762 expressing IFN-gamma elicits antitumor CD4+ MHC class II-restricted T cells that are cytolytic in vitro and tumoricidal in vivo
Frey AB
1995 May 1;154(9):4613-4622, Journal of immunology
— id: 12776, year: 1995, vol: 154, page: 4613, stat: Journal Article,

Expression of activated H-rasval12 in nontumorigenic and non-cross-reactive syngeneic cells induces tumor antigens cross-reactive with rat mammary adenocarcinoma 13762
Frey AB; Cestari S
1995 Nov 15;155(10):4783-4789, Journal of immunology
— id: 6825, year: 1995, vol: 155, page: 4783, stat: Journal Article,

Single exposure of mice to Borrelia burgdorferi elicits immunoglobulin G antibodies characteristic of secondary immune response without production of interleukin-4 by immune T cells
Frey AB; Rao TD
1995 Jul;63(7):2596-2603, Infection & immunity
— id: 6623, year: 1995, vol: 63, page: 2596, stat: Journal Article,

Apparent split tolerance of CD8+ T cells from beta 2-microglobulin-deficient (beta 2m-/-) mice to syngeneic beta 2m+/+ cells
Jhaver KG; Rao TD; Frey AB; Vukmanovic S
1995 Jun 15;154(12):6252-6261, Journal of immunology
— id: 7900, year: 1995, vol: 154, page: 6252, stat: Journal Article,

Protective resistance to experimental Borrelia burgdorferi infection of mice by adoptive transfer of a CD4+ T cell clone
Rao TD; Frey AB
1995 May;162(2):225-234, Cellular immunology
— id: 12773, year: 1995, vol: 162, page: 225, stat: Journal Article,

T<sub>h</sub>1 T cell immune response is produced in mice either by infection or immunization with <i>Borrelia burgdorferi</i>
Rao, T. D.; Frey, A. B.
1995 ;5(1):27-39, Immunology & infectious diseases
— id: 98792, year: 1995, vol: 5, page: 27, stat: Journal Article,

Rat adenocarcinoma 13762 expresses tumor rejection antigens but tumor-bearing animals exhibit tumor-specific immunosuppression
Frey AB; Appleman LJ
1993 Nov;69(2):223-233, Clinical immunology & immunopathology
— id: 13053, year: 1993, vol: 69, page: 223, stat: Journal Article,

Regulated replication of an episomal simian virus 40 origin plasmid in COS7 cells
Chittenden T; Frey A; Levine AJ
1991 Nov;65(11):5944-5951, Journal of virology
— id: 55867, year: 1991, vol: 65, page: 5944, stat: Journal Article,

Differential expression of MAG isoforms during development
Pedraza L; Frey AB; Hempstead BL; Colman DR; Salzer JL
1991 Jun;29(2):141-148, Journal of neuroscience research
— id: 14013, year: 1991, vol: 29, page: 141, stat: Journal Article,

PROTEIN ZERO OF PERIPHERAL-NERVE MYELIN - BIOSYNTHESIS, MEMBRANE INSERTION, AND EVIDENCE FOR HOMOTYPIC INTERACTION
Durso, D; Brophy, PJ; Staugaitis, SM; Gillespie, CS; Frey, AB; Stempak, JG; Colman, DR
1990 Mar;4(3):449-460, Neuron
— id: 32091, year: 1990, vol: 4, page: 449, stat: Journal Article,

Epstein-Barr virus DNA replication
Frey A; Chittenden T; Levine AJ
1989 ;144:227-232, Current topics in microbiology & immunology
— id: 55869, year: 1989, vol: 144, page: 227, stat: Journal Article,

p53-plus-ras-transformed rat embryo fibroblasts express tumor-specific transplantation antigen activity which is shared by independently transformed cells
Frey AB; Levine AJ
1989 Dec;63(12):5440-5444, Journal of virology
— id: 55868, year: 1989, vol: 63, page: 5440, stat: Journal Article,

Purification of complexes of nuclear oncogene p53 with rat and Escherichia coli heat shock proteins: in vitro dissociation of hsc70 and dnaK from murine p53 by ATP
Clarke CF; Cheng K; Frey AB; Stein R; Hinds PW; Levine AJ
1988 Mar;8(3):1206-1215, Molecular & cellular biology
— id: 55870, year: 1988, vol: 8, page: 1206, stat: Journal Article,

Determination of the membrane topology of the phenobarbital-inducible rat liver cytochrome P-450 isoenzyme PB-4 using site-specific antibodies
De Lemos-Chiarandini C; Frey AB; Sabatini DD; Kreibich G
1987 Feb;104(2):209-219, Journal of cell biology
— id: 18421, year: 1987, vol: 104, page: 209, stat: Journal Article,

Immunological evidence for the association of p53 with a heat shock protein, hsc70, in p53-plus-ras-transformed cell lines
Hinds PW; Finlay CA; Frey AB; Levine AJ
1987 Aug;7(8):2863-2869, Molecular & cellular biology
— id: 55871, year: 1987, vol: 7, page: 2863, stat: Journal Article,

3-(Trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine photolabels a substrate-binding site of rat hepatic cytochrome P-450 form PB-4
Frey AB; Kreibich G; Wadhera AB; Clarke L; Waxman DJ
1986 Aug 26;25(17):4797-4803, Biochemistry
— id: 18423, year: 1986, vol: 25, page: 4797, stat: Journal Article,

The structure of phenobarbital-inducible rat liver cytochrome P-450 isoenzyme PB-4. Production and characterization of site-specific antibodies
Frey AB; Waxman DJ; Kreibich G
1985 Dec 5;260(28):15253-15265, Journal of biological chemistry
— id: 18424, year: 1985, vol: 260, page: 15253, stat: Journal Article,

Induction of cytochrome P-450 isozymes in rat hepatoma-derived cell cultures
Frey AB; Rosenfeld MG; Dolan WJ; Adesnik M; Kreibich G
1984 Aug;120(2):169-180, Journal of cellular physiology
— id: 18429, year: 1984, vol: 120, page: 169, stat: Journal Article,

Studies on the subunit composition of rat liver glutathione S-transferases
Frey AB; Friedberg T; Oesch F; Kreibich G
1983 Sep 25;258(18):11321-11325, Journal of biological chemistry
— id: 18430, year: 1983, vol: 258, page: 11321, stat: Journal Article,

Synthesis and incorporation of myelin polypeptides into CNS myelin
Colman DR; Kreibich G; Frey AB; Sabatini DD
1982 Nov;95(2 Pt 1):598-608, Journal of cell biology
— id: 18436, year: 1982, vol: 95, page: 598, stat: Journal Article,

Studies on the biosynthesis of microsomal membrane proteins. Site of synthesis and mode of insertion of cytochrome b5, cytochrome b5 reductase, cytochrome P-450 reductase and epoxide hydrolase
Okada Y; Frey AB; Guenthner TM; Oesch F; Sabatini DD; Kreibich G
1982 Feb;122(2):393-402, European journal of biochemistry
— id: 18440, year: 1982, vol: 122, page: 393, stat: Journal Article,

BIOSYNTHESIS OF CNS MYELIN MEMBRANE-PROTEINS
Colman, DR; Kreibich, G; Frey, AB; Sabatini, DD
1981 ;91(2):A404-A404, Journal of cell biology
— id: 30541, year: 1981, vol: 91, page: A404, stat: Journal Article,

Location of a gelatin-binding region of human plasma fibronectin
Furie MB; Frey AB; Rifkin DB
1980 May 25;255(10):4391-4394, Journal of biological chemistry