Published on NYU Langone Medical Center (http://www.med.nyu.edu)

Home > Printer-friendly > Printer-friendly

Printer-friendly

Printer-friendly

Biosketch / Results /

Ramanuj Dasgupta, PhD

Associate Professor; Scientific Director - RNAi Facility
Departments of Biochemistry and Molecular Pharmacology [1] and NYU Cancer Institute (Research-Fac) [2]

Contact Info

Address
522 First Avenue
NYU School of Medicine Floor 12th Room 1211
Smilow Research Building
New York, NY 10016

212-263-9247, 212-263-9226
Ramanuj.Dasgupta@nyumc.org

[3]

« Back to Results [7]

Education

— University of Chicago, Graduate Education

« Back to Results [7]

Research Interests

The Wnt/Wingless (Wg) pathway is one of a core set of evolutionarily conserved signaling pathways that regulates many aspects of animal development. Aberrant Wnt signaling has been linked to human disease, such as cancers of the intestine, stomach, breast, liver, and skin. Mutations in the Wnt genes or in those that encode regulators of this pathway can cause devastating birth defects, including debilitating abnormalities of the central nervous system, axial skeleton, limbs, and occasionally other organs. The focus of my laboratory is to integrate a variety of functional genomic and proteomic high-throughput screens to generate a global picture of how the Wnt signaling pathway is regulated at a molecular level. We are also interested in understanding how components of the Wnt pathway may interact with other signal transduction cascades during development and disease. We are using a newly developed technology, called RNA-interference (RNAi) to systematically knockdown gene function on a genome scale and testing the effects (phenotype) of reduced gene function on cells and in the developing embryo. The RNAi screens are used to assign new function to genes in the context of the Wnt pathway. Finally, an important aspect of our research will be the combination of RNAi technology with small molecule screens in the hope of isolating more specific drug targets for the Wnt pathway. Though identifying specific targets of small molecules is always a challenge, our approach of comparing and integrating information about specific phenotypes from the RNAi and the small molecule screens, should aid in making target identification faster. This approach will allow us to not only propose new testable hypotheses about the mechanisms of the regulators of the Wnt-signaling pathway, but also in drug discovery with relevance to treatment and prevention of Wnt pathway-related diseases. The Wnt/Wingless (Wg) pathway is one of a core set of evolutionarily conserved signaling pathways that regulates many aspects of animal development. Aberrant Wnt signaling has been linked to human disease, such as cancers of the intestine, stomach, breast, liver, and skin. Mutations in the Wnt genes or in those that encode regulators of this pathway can cause devastating birth defects, including debilitating abnormalities of the central nervous system, axial skeleton, limbs, and occasionally other organs. The focus of my laboratory is to integrate a variety of functional genomic and proteomic high-throughput screens to generate a global picture of how the Wnt signaling pathway is regulated at a molecular level. We are also interested in understanding how components of the Wnt pathway may interact with other signal transduction cascades during development and disease. We are using a newly developed technology, called RNA-interference (RNAi) to systematically knockdown gene function on a genome scale and testing the effects (phenotype) of reduced gene function on cells and in the developing embryo. The RNAi screens are used to assign new function to genes in the context of the Wnt pathway. Finally, an important aspect of our research will be the combination of RNAi technology with small molecule screens in the hope of isolating more specific drug targets for the Wnt pathway. Though identifying specific targets of small molecules is always a challenge, our approach of comparing and integrating information about specific phenotypes from the RNAi and the small molecule screens, should aid in making target identification faster. This approach will allow us to not only propose new testable hypotheses about the mechanisms of the regulators of the Wnt-signaling pathway, but also in drug discovery with relevance to treatment and prevention of Wnt pathway-related diseases.

« Back to Results [7]

All data from NYU Health Sciences Library Faculty Bibliography — -

Contact:
http://hsl.med.nyu.edu/faculty-bibliography-search [8]

A membrane-associated beta-catenin/Oct4 complex correlates with ground-state pluripotency in mouse embryonic stem cells
Faunes, Fernando; Hayward, Penelope; Descalzo, Silvia Munoz; Chatterjee, Sujash S; Balayo, Tina; Trott, Jamie; Christoforou, Andrew; Ferrer-Vaquer, Anna; Hadjantonakis, Anna-Katerina; Dasgupta, Ramanuj; Arias, Alfonso Martinez
2013 Mar;140(6):1171-1183, Development
— id: 249552, year: 2013, vol: 140, page: 1171, stat: Journal Article,

Regulation of pluripotency and cellular reprogramming by the ubiquitin-proteasome system
Buckley, Shannon M; Aranda-Orgilles, Beatriz; Strikoudis, Alexandros; Apostolou, Effie; Loizou, Evangelia; Moran-Crusio, Kelly; Farnsworth, Charles L; Koller, Antonius A; Dasgupta, Ramanuj; Silva, Jeffrey C; Stadtfeld, Matthias; Hochedlinger, Konrad; Chen, Emily I; Aifantis, Iannis
2012 Dec;11(6):783-798, Cell Stem Cell
— id: 202132, year: 2012, vol: 11, page: 783, stat: Journal Article,

Antagonistic Effect of Small-molecule Inhibitors of Wnt/beta-catenin in Multiple Myeloma
Narayanan, Bhagavathi A; Doudican, Nicole A; Park, Jeesun; Xu, Dazhong; Narayanan, Narayanan K; Dasgupta, Ramanuj; Mazumder, Amitabha
2012 Nov;32(11):4697-4707, Anticancer research
— id: 182552, year: 2012, vol: 32, page: 4697, stat: Journal Article,

INHIBITION OF THE WNT PATHWAY IN COMBINATION WITH TRADITIONAL CHEMOTHERAPY INCREASES CHEMOSENSITIVITY IN T- AND B- ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINES
Romanos-Sirakis, Eleny; Morrison, Debra; Raetz, Elizabeth; DasGupta, Ramanuj; Carroll, William
2012 JUL;58(7):1017-1018, Pediatric blood & cancer
— id: 166838, year: 2012, vol: 58, page: 1017, stat: Journal Article,

A Systematic Screen for Micro-RNAs Regulating the Canonical Wnt Pathway
Anton, Roman; Chatterjee, Sujash S; Simundza, Julia; Cowin, Pamela; Dasgupta, Ramanuj
2011 ;6(10):e26257-e26257, PLoS ONE
— id: 140537, year: 2011, vol: 6, page: e26257, stat: Journal Article,

Feature Article: From the Cover: An RNAi-based chemical genetic screen identifies three small-molecule inhibitors of the Wnt/wingless signaling pathway
Gonsalves, Foster C; Klein, Keren; Carson, Brittany B; Katz, Shauna; Ekas, Laura A; Evans, Steve; Nagourney, Robert; Cardozo, Timothy; Brown, Anthony M C; Dasgupta, Ramanuj
2011 Apr 12;108(15):5954-5963, Proceedings of the National Academy of Sciences of the United States of America
— id: 130910, year: 2011, vol: 108, page: 5954, stat: Journal Article,

Yan, an ETS-domain transcription factor, negatively modulates the Wingless pathway in the Drosophila eye
Olson, Emily R; Pancratov, Raluca; Chatterjee, Sujash S; Changkakoty, Binita; Pervaiz, Zeeshan; Dasgupta, Ramanuj
2011 ;12(10):1047-1054, EMBO reports
— id: 138112, year: 2011, vol: 12, page: 1047, stat: Journal Article,

Postgenomic technologies targeting the Wnt signaling network
Pancratov, Raluca; Dasgupta, Ramanuj
2011 Nov;3(6):649-665, Wiley interdisciplinary reviews. Systems biology & medicine
— id: 138103, year: 2011, vol: 3, page: 649, stat: Journal Article,

Functional genomic approaches targeting the wnt signaling network
Dasgupta, Ramanuj
2009 Jul;10(7):620-631, Current drug targets
— id: 100675, year: 2009, vol: 10, page: 620, stat: Journal Article,

Bili inhibits Wnt/beta-catenin signaling by regulating the recruitment of axin to LRP6
Kategaya, Lorna S; Changkakoty, Binita; Biechele, Travis; Conrad, William H; Kaykas, Ajamete; Dasgupta, Ramanuj; Moon, Randall T
2009 ;4(7):e6129-e6129, PLoS ONE
— id: 101871, year: 2009, vol: 4, page: e6129, stat: Journal Article,

High-throughput RNAi screen in Drosophila
DasGupta, Ramanuj; Gonsalves, Foster C
2008 ;469:163-184, Methods in molecular biology
— id: 92186, year: 2008, vol: 469, page: 163, stat: Journal Article,

Function of the wingless signaling pathway in Drosophila
Gonsalves, Foster C; DasGupta, Ramanuj
2008 ;469:115-125, Methods in molecular biology
— id: 92184, year: 2008, vol: 469, page: 115, stat: Journal Article,

A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila
DasGupta, Ramanuj; Nybakken, Kent; Booker, Matthew; Mathey-Prevot, Bernard; Gonsalves, Foster; Changkakoty, Binita; Perrimon, Norbert
2007 Sep 28;8(9):R203-R203, Genome biology
— id: 74273, year: 2007, vol: 8, page: R203, stat: Journal Article,

Functional genomics reveals genes involved in protein secretion and Golgi organization
Bard, Frederic; Casano, Laetitia; Mallabiabarrena, Arrate; Wallace, Erin; Saito, Kota; Kitayama, Hitoshi; Guizzunti, Gianni; Hu, Yue; Wendler, Franz; Dasgupta, Ramanuj; Perrimon, Norbert; Malhotra, Vivek
2006 Feb 2;439(7076):604-607, Nature
— id: 63618, year: 2006, vol: 439, page: 604, stat: Journal Article,

Drosophila Wnt/Fz pathways
DasGupta, Ramanuj; Boutros, Michael; Perrimon, Norbert
2005 May 10;2005(283):cm5-cm5, Science's STKE
— id: 59510, year: 2005, vol: 2005, page: cm5, stat: Journal Article,

Functional genomic analysis of the Wnt-wingless signaling pathway
DasGupta, Ramanuj; Kaykas, Ajamete; Moon, Randall T; Perrimon, Norbert
2005 May 6;308(5723):826-833, Science
— id: 59511, year: 2005, vol: 308, page: 826, stat: Journal Article,

Notch modulates Wnt signalling by associating with Armadillo/beta-catenin and regulating its transcriptional activity
Hayward, Penny; Brennan, Keith; Sanders, Phil; Balayo, Tina; DasGupta, Ramanuj; Perrimon, Norbert; Martinez Arias, Alfonso
2005 Apr;132(8):1819-1830, Development
— id: 59512, year: 2005, vol: 132, page: 1819, stat: Journal Article,

The Wingless morphogen gradient is established by the cooperative action of Frizzled and Heparan Sulfate Proteoglycan receptors
Baeg, Gyeong-Hun; Selva, Erica M; Goodman, Robyn M; Dasgupta, Ramanuj; Perrimon, Norbert
2004 Dec 1;276(1):89-100, Developmental biology (Orlando)
— id: 59513, year: 2004, vol: 276, page: 89, stat: Journal Article,

Using RNAi to catch Drosophila genes in a web of interactions: insights into cancer research
Dasgupta, Ramanuj; Perrimon, Norbert
2004 Nov 1;23(51):8359-8365, Oncogene
— id: 59514, year: 2004, vol: 23, page: 8359, stat: Journal Article,

Links between signal transduction, transcription and adhesion in epithelial bud development
Jamora, Colin; DasGupta, Ramanuj; Kocieniewski, Pawel; Fuchs, Elaine
2003 Mar 20;422(6929):317-322, Nature
— id: 59515, year: 2003, vol: 422, page: 317, stat: Journal Article,

A developmental conundrum: a stabilized form of beta-catenin lacking the transcriptional activation domain triggers features of hair cell fate in epidermal cells and epidermal cell fate in hair follicle cells
DasGupta, Ramanuj; Rhee, Horace; Fuchs, Elaine
2002 Jul 22;158(2):331-344, Journal of cell biology
— id: 59516, year: 2002, vol: 158, page: 331, stat: Journal Article,

Source URL: http://www.med.nyu.edu/biosketch/dasgur01

Links:
[1] http://www.med.nyu.edu/pharmacology/
[2] http://nyuci.org/
[3] https://campusconnectioncmnpt.nyumc.org/psp/hrprod/EMPLOYEE/HRMS/c/ROLE_EMPLOYEE.HR_EE_PERS_INFO.GBL
[4] http://www.med.nyu.edu/dasgur01#education
[5] http://www.med.nyu.edu/dasgur01#research
[6] http://www.med.nyu.edu/dasgur01#publications
[7] http://www.med.nyu.edu/dasgur01
[8] http://hsl.med.nyu.edu/facult