Compartmentalized Signaling
Ras activation is seen after 3 minutes on the plasma membrane and after 40 minutes following stimulation with EGF of COS-1 cells transfected with the GFP-RBD biosensor and H-Ras. more detail
Our biosensor for activated Ras consists of the Ras binding domain (RBD) of Raf-1 fused to GFP.
more detail
Ras activation is seen after 3 minutes on the plasma membrane and after 40 minutes following stimulation with EGF of COS-1 cells transfected with the GFP-RBD biosensor and H-Ras. more detail
Our biosensor for activated Ras consists of the Ras binding domain (RBD) of Raf-1 fused to GFP.
more detail
Using genetically encoded fluorescent probes, we have discovered that Ras signals from intracellular membranes as well as from the plasma membrane. We now seek to understand the biological significance of compartmentalized signaling.