the philips lab
departments of medicine, cell biology and pharmacology, NYU cancer institute, Division of Rheumatology, NYU School of Medicine
Compartmentalized Signaling
Compartmentalized Signaling
Ras activation is seen after 3 minutes on the plasma membrane and after 40 minutes following stimulation with EGF of COS-1 cells transfected with the GFP-RBD biosensor and H-Ras. more detail
Compartmentalized Signaling
Our biosensor for activated Ras consists of the Ras binding domain (RBD) of Raf-1 fused to GFP.
more detail

Using genetically encoded fluorescent probes, we have discovered that Ras signals from intracellular membranes as well as from the plasma membrane.  We now seek to understand the biological significance of compartmentalized signaling.