the philips lab
departments of medicine, cell biology and pharmacology, NYU cancer institute, Division of Rheumatology, NYU School of Medicine
Ras Activation in T Cells
Ras Activation in T Cells
Ras activation on the Golgi of T cells is mediated by the RasGRP1 guanine nucleotide exchange factor. Ras activation on the plasma membrane is kept in check by the GAP CAPRI. more detail
Ras Activation in T Cells
CD4+ splenocytes from a BALB/c mouse were transduced by nucleofection with CFP-H-Ras and YFP-RBD and stimulated (bottom) by cross-linking the T cell receptor.  Accumulation of the YFP-RBD biosensor is seen only on the Golgi apparatus. more detail

We have discovered that the Golgi apparatus is the primary platform upon which RasGRP1 activates Ras in lymphocytes.  We now seek to understand what are the biological advantages of endomembrane Ras signaling in immune cells and the role that Ras signaling plays in autoimmunity.