Microscopy: Announcements

Zeiss Lightsheet

Lightsheet microscopy is excellent for transparent live organisms such as embryos and zebrafish and for small cleared organs.  It complements confocal microscopy by imaging optical sections of larger tissues.

The lightsheet microscope here works with living samples in water or media and with fixed and cleared samples such as CLARITY.  Our system is aqueous media only.

With lasers at 405, 488, 568, and 638 nm, this system images most genetically encoded fluorescent proteins and other popular fluorescent probes.  Magnification is at 5X or 20X.  Images are collected with two 1920 X 1920 pixels sCMOS cameras.

Please contact Microscopy Core for further information about this microscope.

Zeiss LSM 88O with AiryScan

This microscope has many imaging modes, far more than the typical confocal. It has a 32 channel detector for superior spectral detection and software to separate probes that have very similar spectra, such as GFP and YFP. It also has a wide range of lasers from 405 nm through 638 nm. In addition to the standard lines for blue, green, red, far-red imaging, it has a 440 nm laser for CFP and a 594 nm laser for Alexa 594 or other new red probes.

The microscope has an additional AiryScan detector that can boost spatial resolution up to 2X finer than standard confocal. AiryScan uses a 32 channel detector in a hexagonal array to collect all light from the zero and first order rings of an Airy pattern with spatial distribution preserved. The preserves more light than standard pinhole confocal and allows for additional computation to improve spatial resolution.

Two GaAsP detectors with filters for green-red or CFP-YFP can be used for time resolved FLIM with the pulsed 440 nm laser or FCS with CW lasers. Other techniques may be available too. Of course, these detectors may also be used for ultra-low light confocal too.

Please contact Microscopy Core for further information about this microscope.