Immunohistochemistry (IHC) Core
The Immunohistochemistry (IHC) Core, a component of the Experimental Pathology Shared Resource, serves as an immunohistochemical antibody repository with a catalog of over 1,400 antibodies. The IHC Core Laboratory develops and optimizes immunohistochemical chomogenic and immunofluorescent immunohistochemical protocols for immunohistochemistry of commercial or proprietary antibodies for the collection of IHC data in fresh, frozen, fixed human or animal tissues and cells. Once optimized, samples may be run on any of the IHC Core Laboratories: two Ventana NEXES, one Discovery, and one Discovery XT automated immunohistochemical staining instuments (90 slide total capacity). The IHC core uses human and animal tissue micro-arrays for optimization, validation, and verification and can employ a wide variety of retrieval and blocking techniques for protocol optimization and development. For chromogenic IHC, the Core Laboratory offers both streptavidin-biotin and multimer Horseradish Peroxidase and Alkaline Phosphatase detection. For immunofluorescence, the Core Laboratory has a variety of species-fluorophore combinations available for detection. The IHC Core Laboratory also prepares and creates Tissue Microarrays from both human archival and animal FFPE tissues. Archival human tissues for creating Tissue Microarrays are procured through the Human Specimen Resource Center. Animal tissue is collected and processed through the Histopathology Core. Please see the links below for additional information.
Phone: 646-501-6934, 347-712-0897
NYU Langone Medical Center
550 First Avenue
New York, NY 10016