Immunohistochemistry (IHC) Core
The Immunohistochemistry (IHC) Core, a component of the Experimental Pathology Shared Resource, serves as an immunohistochemical antibody repository with a catalog of over 1,500 different antibodies. The IHC Core Laboratory optimizes and validates commercial or proprietary antibodies for chromogenic or immunoflorescent, immunohistochemistry/immunocytochemistry of fresh, frozen, fixed human or animal cells and tissues. The Core Laboratory operates one Ventana Discovery and 2 Discovery XT automated IHC platforms with 80 side capacity. The core uses human and animal tissue micro-arrays for immunohistochemical optimization, validation, and verification and can employ a wide variety of off- and online retrieval methods as well as blocking strategies for protocol optimization and biomarker development. The Core Laboratory offers: traditional streptavidin-biotin, multimer and hapten amplified horseradish peroxidase (brown or purple) and alkaline phosphatase (blue or red) detection systems with double label capabilities. For immunofluorescence, the laboratory has a variety of alexa fluor conjugated, species-specific secondary antibodies available as well as horseradish peroxidase driven FITC and Rhodamine detection. The IHC Core Laboratory prepares and constructs Tissue Microarrays from both human and animal FFPE tissues blocks. Archival human tissues for creating tissue microarrays are procured through the Center for Biospecimen Research and Development. For histopathology services, please contact the Histopathology Core Lab. For additional information on IHC services please see the links below.
Phone: 646-501-6934, 347-712-0897
NYU Langone Medical Center
550 First Avenue
New York, NY 10016