Immunohistochemistry (IHC) Core
The Immunohistochemistry (IHC) Core, a component of the Experimental Pathology Shared Resource, serves as an immunohistochemical antibody repository with a catalog of over 1,500 antibodies (the IHC core is currently looking at options to digitize its catalog so that investigators can search the database directly). The IHC Core Laboratory optimizes commercial or proprietary antibodies for chomogenic or immunofluorescent immunohistochemistry. Established antibodies and protocols are immediately available for the collection of IHC data in fresh, frozen, fixed human or animal tissues and cells. Once optimized, samples may be run on any of the IHC Core Laboratories Ventana Discovery XT or Discovery automated immunohistochemical staining platforms (80 slide total capacity). The IHC core uses human and animal tissue micro-arrays for immunohistochemistry optimization, validation, and verification and can employ a wide variety of retrieval and blocking techniques for protocol optimization and biomarker development. For chromogenic IHC, the Core Laboratory offers streptavidin-biotin, multimeric and amplified horseradish peroxidase (brown) as well as alkaline phosphatase (blue or red) detection. For immunofluorescence, the Core Laboratory has a variety of species-fluorophore combinations available for detection. The IHC Core Laboratory also prepares and constructs Tissue Microarrays from both human archival and animal FFPE tissues. Archival human tissues for creating Tissue Microarrays are procured through the Human Specimen Resource Center. Animal tissue is collected and processed through the Histopathology Core. Please see the links below for additional information.
Phone: 646-501-6934, 347-712-0897
NYU Langone Medical Center
550 First Avenue
New York, NY 10016