pH Regulation in the Nervous System
An experimental arrangement used to study excitatory synaptic currents (*) and corresponding pH shifts in a hippocampal slice. Intracellular pH of an astrocyte (pHi) and extracellular pH (pHo) increase rapidly during stimulation. Microelectrode and optical recording methods permit investigation of the H+ fluxes.
Mitchell Chesler, MD, PhD
Dr. Chesler is Professor of Neurosurgery and Physiology and Neuroscience. His laboratory investigates regulation of pH in the nervous system. The focus of this research is how H+ is rapidly transported during neuronal activity, and how glial cells regulate pH. An additional focus of investigation is how regionally-specific changes in pH contribute to cellular signaling and CNS maturation and development.
Publications
- Fedirko N, Svichar N, Chesler M. Fabrication and use of high-speed, concentric h+- and Ca2+-selective microelectrodes suitable for in vitro extracellular recording. J Neurophysiol 96:919-924, 2006.
- Tong CK, Chen K, Chesler M. Kinetics of activity-evoked pH transients and extracellular pH buffering in rat hippocampal slices. J Neurophysiol 95:3686-3697, 2006.
- Shah GN, Ulmasov B, Waheed A, Becker T, Makani S, Svichar N, Chesler M, Sly WS. Carbonic anhydrase IV and XIV knockout mice: roles of the respective carbonic anhydrases in buffering the extracellular space in brain. Proc Natl Acad Sci U S A 102:16771-16776, 2005.
An experimental arrangement used to study excitatory synaptic currents (*) and corresponding pH shifts in a hippocampal slice. Intracellular pH of an astrocyte (pHi) and extracellular pH (pHo) increase rapidly during stimulation. Microelectrode and optical recording methods permit investigation of the H+ fluxes.
