Weiser Lab - Microbiology
Jeffrey N. Weiser, M.D.
Professor and Chair
Medical Science Building, Room 256
550 First Avenue, New York, NY 10016
Office: (212) 263-1080
Fax: (212) 263-8276
Lab: (212) 263-1081
B.S. (Biological Sciences) Stanford University, 1979.
M.D. Harvard University, 1984.
Resident in Pediatrics, University of Washington, 1984-87
Research Fellow, Oxford University, 1987-89
Research Associate, Rockefeller University, 1989-92
Professor of Microbiology and Pediatrics, University of Pennsylvania School of Medicine (through 12/31/14)
Professor of Microbiology, New York University School School of Medicine (2015 - )
Description of Weiser Lab Research:
The Weiser lab investigates the molecular basis of host-pathogen interaction for agents that reside in and infect the human respiratory tract. Most studies focus on the pathogenesis of Streptococcus pneumoniae because of its prominence as a cause of acute respiratory tract infection. Other pathogens under investigation in the laboratory have included Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, and Influenza A. Many projects focus on colonization of the mucosal surface of the upper airway, the initial step in the disease process. Both host and bacterial factors affecting colonization are under investigation. In general these studies utilize bacterial genetics to examine effects of specific microbial genes together with mouse models of colonization that allow for the use of genetically-modified hosts.
On-going projects examine:
How colonizing microbes stimulate and evade innate and adaptive immune clearance mechanisms.
Mechanisms that facilitate interspecies competition within a host.
Mechanisms of competition between co-colonizing pneumococci.
Effects of influenza co-infection on bacterial colonization.
Pneumococcal interactions with neutrophils/macrophages and mechanisms to evade clearance by professional phagocytes.
The role of phosphorycholine, a surface constituent of many respiratory tract inhabitants, in pathogenesis.
Host and bacterial factors affecting shedding and host to host transmission.
Systemic effects on innate immunity of the colonizing flora (microbiota).
Selected Publications (since 2010):
Clearance of Pneumococcal Colonization in Infants Is Delayed through Altered Macrophage Trafficking.
Siegel SJ, Tamashiro E, Weiser JN.
PLoS Pathog. 2015 Jun 24;11(6):e1005004. doi: 10.1371/journal.ppat.1005004. eCollection 2015 Jun.
TLR2 signaling decreases transmission of Streptococcus pneumoniae by limiting bacterial shedding in an infant mouse Influenza A co-infection model.
Richard AL, Siegel SJ, Erikson J, Weiser JN.
PLoS Pathog. 2014 10:e1004339.
Shielding of a lipooligosaccharide IgM epitope allows evasion of neutrophil-mediated killing of an invasive strain of nontypeable Haemophilus influenzae.
Langereis JD, Weiser JN.
MBio. 2014 5:e01478-14.
Influenza promotes pneumococcal growth during coinfection by providing host sialylated substrates as a nutrient source.
Siegel SJ, Roche AM, Weiser JN. Cell Host Microbe. 2014 16:55-67.
Antibody blocks acquisition of bacterial colonization through agglutination.
Roche AM, Richard AL, Rahkola JT, Janoff EN, Weiser JN.
Mucosal Immunol. 2014 Jun 25. doi: 10.1038/mi.2014.55. [Epub ahead of print]
Macrophage migration inhibitory factor promotes clearance of pneumococcal colonization.
Das R, LaRose MI, Hergott CB, Leng L, Bucala R, Weiser JN. J Immunol. 2014 193:764-72.
Protection from the acquisition of Staphylococcus aureus nasal carriage by cross-reactive antibody to a pneumococcal dehydrogenase.
Lijek Rebeccah S, Luque Santiago L, Liu Qian, Parker Dane, Bae Taeok, Weiser Jeffrey N: Proceedings of the National Academy of Sciences of the United States of America 2012 109: 13823-8
Minimization of bacterial size allows for complement evasion and is overcome by the agglutinating effect of antibody.
Dalia AB, Weiser JN.
Cell Host Microbe. 2011 10(5):486-96.
Nod2 sensing of lysozyme-digested peptidoglycan promotes macrophage recruitment and clearance of S. pneumoniae colonization in mice.
Davis KM, Nakamura S, Weiser JN. J Clin Invest. 2011 121:3666-76.
Synergistic stimulation of type I interferons during influenza virus coinfection promotes Streptococcus pneumoniae colonization in mice.
Nakamura S, Davis KM, Weiser JN. J Clin Invest. 2011 121:3657-65.
Within-host competition drives selection for the capsule virulence determinant of Streptococcus pneumoniae.
Lysenko ES, Lijek RS, Brown SP, Weiser JN. Current Biology 2010 20:1222-6.
Recognition of peptidoglycan from the microbiota by Nod1 enhances systemic innate immunity.
Clarke TB, Davis KM, Lysenko ES, Zhou AY, Yu Y, Weiser JN. Nat Med. 2010 16:228-31.
Shigeto Hamaguchi Ph.D. – Visiting Scholar
Pam Shen Ph.D. – Postdoctoral Fellow
Ammar Zafar Ph.D. – Research Scientist
Tonia Zangari Ph.D. – Postdoctoral Fellow
Jennifer Pagano – Executive Assistant
Weiser Lab 2016