Core Facilities by Research Center
Center for AIDS Research
Clinical Core: Biostatistics and Epidemiology
Patient-based research: epidemiological and biostatistical consultation, biomathematical modeling, and data management.
Clinical Core: Specimen Management
Processing and storage of biological samples from HIV-infected patients and uninfected controls, cataloging and distribution of information on stored specimens, procurement of fresh biological samples for HIV/AIDS studies.
Flow Cytometry
Innovative flow measurements, 3-color surface phenotyping of lymphocytes, training and consultation on phenotyping methods.
Immunology Core
Immunologic assays; testing of polyclonal sera as well as human, primate and rodent anti-HIV mAbs for neutralizing activity against HIV primary isolates and for their ability to bind to intact virions and to infected cells; provision of human anti-HIV mAbs; frozen stocks of more than 70 mycoplasma-free cell lines producing human anti-HIV mAbs.
Virology Core
Bulk and quantitative HIV culture, measurement, resistance testing, phenotype analysis , and genotype analysis using heteroduplex mobility assay and sequencing; HIV RNA in plasma or culture fluids by the Roche RT-PCR method; and a licensed virology lab which detects the presence of herpes viruses (HSV, CMV, HHV6, VZV, and EBV) by culture and/or PCR, as well as other viruses such as HCV and JC by PCR.
General Clinic Research Center (GCRC)
Research Nursing Staff/Nursing Services
Trained research nurses with technical expertise including monitoring, critical assessment, and teaching of patients.
Core Lab
Molecular biology support for GCRC clinical research protocols, training of clinical research fellows and medical students in the laboratory methods of patient-oriented research.
BioInformatics Core
Consulting and software for data management on GCRC projects involving DNA or protein sequences, gene expression microarrays, multilocus genotyping/SNPs, or proteomics.
Biostatistics Core
Statistical consultation and collaboration to GCRC investigators in all stages of the clinical research process for approved protocols. Protocol development and review through conduct, analysis, and reporting of results.
Informatics Core
Informatics policies (HIPAA, privacy, security); best practices; protocol development; accessing services; database design, development and maintenance and programming for automated data uploads or extractions; and reporting, coding, un-blinding, de-identifying and archiving data.
Research Subject Safety Office
Ensurance of safety and ethical conduct, with protection of human subjects accorded the highest priority.
Skirball Institute of Biomolecular Medicine
DNA Sequencing Facility
DNA sequencing performed with Applied Boisystems 3730 DNA Analyzer; in-person consulting for sequencing strategy planning and troubleshooting.
Electron Microscopy
Wide spectrum of microscopy equipment, technical assistance in sample
preparation and image acquisition. CM 12 transmission electron microscope,
CM 200 transmission electron microscope with cryo holder, Leica
Ultracut UCT ultramicrotome, Sorvall ultramicrotome, Leica glass
knife-makers, Denton Vacuum carbon coating unit, Carl Zeiss inverted
microscope, Zeiss light microscope. Instruction, consultation, assistance
and collaboration for the research involving electron microscopy.
Sample preparation, ultramicrotomy, staining, and immuno-gold electron
microscopy.
Media Services
Professional quality large format posters, digital prints, digital production services, fabric poster printing.
Protein Analysis Lab
ALDI-TOF mass spectrometry, Q-TOF mass spectrometry (MS/MS peptide sequencing), Ion Trap mass spectrometry (MS/MS peptide sequencing), proteolysis and identification of proteins from SDS-PAGE gels.
X-Ray Diffraction Lab
Two Rigaku X-ray systems for collecting macromolecular single-crystal diffraction data.
NYU Cancer Institute
Analytical Chemistry
Combined mass spectrometry and metals analysis laboratories. Mass spectrometry (MS) and atomic absorption (AA) spectroscopy; consultative services to NYU investigators in physical and inorganic chemistry, separations science, and spectral interpretation (MS, NMR, UV); training of students and researchers in the use of MS, NMR, and AA.
Biostatistics
Statistical collaboration and consultation in clinical and translational oncology, basic science, cancer epidemiology and prevention, and the core research services. Statistical expertise in research study design, conduct, analysis, interpretation, and reporting of results for clinical trials, laboratory experiments, and observational studies.
Core Clinical Laboratory
Support of immune-modulating antineoplastic agents and cytotoxic therapies, specialized clinical correlates to monitor novel approaches to cancer treatment. Laboratory support for institutional cancer clinical trials, logistical support for trials that require specimen collection and assessment of multiple parameters ranging from specialized tests for patient eligibility to quality control of specialized therapeutic modalities.
Cytogenics
Traditional chromosomal karyotyping of experimental cell lines chromosomal damage analysis, including sister chromatid exchange (SCE) micronucleus quantitation fluorescent in situ hybridization (FISH) technology, including metaphase and interphase FISH.
Experimental Animal and Exposure Core
Facilities, animal care, technical assistance for experiments with rodent species involving delivery of carcinogenic and toxic chemicals, infectious agents, or intervention compounds, using inhalation, systemic, dermal, and oral administration routes of exposure; experimental design and conduct for cancer-related animal studies; segregated housing and health monitoring of animals; necropsy services for animal experiments.
Experimental Pathology
Anatomic pathology: tissues acquisition and banking, histopathology, acquisition of human pathological specimens, processing of human and murine tissues; resources and guidance for investigators; access to immunohistochemistry, murine cytogenetics, laser capture, quantitative morphometry, and the preparation of tissue arrays.
Flow Cytometry & Cell Sorting
DakoCytomation MoFlo, Becton Dickinson LSR system, Becton Dickinson FACScan.
Genomics Facility
Affymetrix Genechip system for gene expression and DNA genotyping analyses, manufacturing of spotted DNA arrays, pyrosequencing and high-throughput quantitative PCR. Experimental designs, standards for DNA array procedures, guidance in data management and analysis.
Histopathology
Histology and comparative pathology service: paraffin, plastic, and cryostat sectioning, preparation of slides for conventional and specialized staining, immunohistochemistry, and in situ hybridization; conventional and specialized slide staining and immunohistochemistry; necropsy services including appropriate tissue fixation required for animal experiments; comprehensive consultation and collaboration on comparative and experimental pathology; basic photomicroscopy services.
Immunohistology
State-of-the-art computer-controlled immunohistochemical system, extensive menu of antibodies (Angiogenesis - CD34, CD31, Factor VIII, FAK, VEGF, HIF-1 alpha; apoptosis - bcl-2, CD30, MDM2, TUNEL, activated caspase 3; cell cycle and related - cyclins D1, E, p16, p21, p27, p53, p57; hematopoietic - CD1a, CD3, CD4, CD8, CD5, CD61, CD99, TIA-1, CD117; hormone and related - estrogen, progesterone, erb-B2, MIB-1; thymidine phosphorylase, CD44, cytokeratins 7, 19, 17, E-cadherin, FHIT, Cox-2, TTF-1, TGF-b, MDR, EGF-R, green fluorescent protein). Development of preliminary data as well as staining of funded research.
Mouse Imaging
Magnetic resonance micro-imaging (µMRI) and ultrasound biomicroscopy (UBM) services to detect and follow tumor progression in mouse models.
Tissue Acquisition & Banking Services
Human tissue sample procurement (collected from excess surgical material) and tissue banking for investigators involved in peer-reviewed grants or institutionally funded projects.
Transgenic Mouse/ES Cell Chimera Facility
Production of transgenic (Tg) mice by zygote injection, production of embryonic stem (ES) cell chimeras, and rederivation of mouse strains.
