Blaser Lab Group

This page provides the most common protocols used in our research laboratory.

The procedures have been sorted and listed according to specific area or topic.

Please direct questions or comments to any member of the laboratory.

Molecular Biology

1. Small Scale Expression of Tagged Recombinant Proteins

2. E. coli Calcium Chloride competent cell protocol and Transformation of Ca++ competent cells

Immunological Procedures

1. ELISA Protocol for Lewis antigen determination in Hp

2. LPS SDS-Urea PAGE for Lewis Western Blot (10%)

3. LPS SDS-Urea PAGE for Lewis Western Blot (15%)

Detection Methods

1. LPS SDS Urea PAGE