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Facility Cores
Analytical Services and Exposure Assessment Facility Core Polina Maciejzcyk, Ph.D., Director The Analytical Services and Exposure Assessment Facility Core consists of two analytical laboratory units and an instrumentation Unit. The Analytical Measurements Laboratory Unit includes specialized instruments for inorganic chemical analysis, organic chemical analysis and flow cytometry. The Exposure Assessment Laboratory Unit houses a variety of highly specialized atmospheric monitoring instruments for analyzing ambient and test atmospheres. The Instrumentation Unit provides shared instrumentation maintenance, repair and fabrication services.
Polina B. Maciejczyk, Ph.D. NYU NIEHS Center The Analytical Services and Exposure Assessment Facility Core provides an array of analytical services that are driven by the scientific needs of the members of the Center. The services provided are:
Personnel Paul Peters is the Exposure Assessment Equipment Unit Manager. He supervises the day-to-day use of all exposure assessment equipment, and manages the records of its use, storage and maintenance. Thomas Darling, the Instrumentation Unit machinist, works with Mr. Gorczynski and all Center investigators to repair, modify, design and create novel or specialized devices, tools, and instruments that are required by the Center investigatorÕs research project. Contact Information: Back to Top >> Other Responsibilities: Dr. Goldberg is also the Director of the Division of Biostatistics in the School of Medicine, the Director of the Biostatistics Shared Resource of the NYU Cancer Institute, the Director of Biostatistics for the NYUSOM General Clinical Research Center, and Director of the Environmental Health Statistics and Bioinformatics Facility of the NIEHS Center at NYU. Dr. Goldberg is a Fellow of the American Statistical Association and a Fellow of the American Association for the Advancement of Science. She has served as President of the Biometric Society (ENAR), as Chair of the Fellows Committee of the American Statistical Association, as Chair of the Statistics Section U of the AAAS, and is currently Chair of the Short Course Committee for the International Biometric Society 2006 Meetings. Dr. Goldberg just completed a term as a Member of the U.S. Food and Drug Administration Vaccines and Related Biological Products Advisory Committee. Research Interests: Survival Analysis; Clinical Trials; Methods for the Analysis of Screening and Diagnostic Tests; Misclassification; Methods for the Analysis of Observational Data; Statistical Methods in Epidemiology; Statistical Genomics. Contact: TEL: (212) 263-0314 FAX: (212)263-8570 NYU NIEHS CenterEnvironmental Health Statistics and Bioinformatics Facility Core PurposeThe aim of the Environmental Health Statistics and Bioinformatics Facility Core is to provide:
Personnel Judith D. Goldberg, Sc.D., Director, is a Professor of Environmental Medicine and the Director of the Division of Biostatistics. Dr. Goldberg is also the Director of the Biostatistics Shared Resource of the NYU Cancer Institute and of the Biostatistics Core of the NYU General Clinical Research Center. Dr. Goldberg has extensive statistical expertise and experience in the areas of design, conduct, and analysis of randomized trials and observational studies, evaluation of disease screening tests, misclassification, survival analysis and censored data methods, statistical methods in epidemiology, and extensive experience in the design, development, and implementation of data management procedures and systems in both academia and industry. She is a Fellow of the American Statistical Association and of the AAAS. Karen Koenig, Ph.D., is an Assistant Professor of Epidemiology and Biostatistics in the Department of Environmental Medicine. She received her Ph.D. in Environmental Health Sciences (Epidemiology and Biostatistics) from NYU in 1989. Since that time, she has provided statistical consulting services to members of the Department, first for the Cancer Center and later for the NIEHS Center. Dr. Koenig is an applied biostatistician and epidemiologist with a strong interest in cancer research. Arthur Nádas, Ph.D., Associate Professor in the Division of Biostatistics in the Department of Environmental Medicine is an experienced mathematical statistician who is actively involved in statistics research. Dr. Nádas, who has been a member of the Facility Core since 1995, has expertise in a number of areas in statistics, including biostatistics, mathematical modeling, pattern recognition, and statistical computing. Yongzhao Shao, Ph.D.,is Associate Professor in the Division of Biostatistics in the Department of Environmental Medicine. He joined NYU School of Medicine in July 2003. He has a strong background in statistical methodology and applications. Dr. Shao’s current research interests include design and analysis of biomedical studies, genetic epidemiology, and statistical genomics. In particular, his current research interests are focused in genetic linkage and association analysis. Dr. Shao is also a member of the NYU Cancer Institute Biostatistics Shared Resource. Ilana Belitskaya-Levy, Ph.D., is an Assistant Professor in the Division of Biostatistics in the Department of Environmental. She joined NYUSOM after receiving her Ph.D. in Statistics from Stanford University in November, 2002. Dr. Belitskaya has research interests in high dimensional data analysis, algorithms for missing data analysis, cluster analysis, data mining, nonparametric statistics, and in particular the applications of statistics for the analysis of large data sets arising in genomics, biology, and medicine, microarrays, and flow cytometry. Dr. Belitskaya is a member of the NYU Cancer Institute Biostatistics Shared Resource and is a statistician for the NYU General Clinical Research Center where she collaborates in the functional genomics program as well as in the clinical and translational research programs. Stuart Brown, Ph.D.,is Associate Professor of Cell Biology and the Director of the Research Computing Facility (RCC) at NYU School of Medicine. The RCC provides the bioinformatics core facility for the School of Medicine. Dr. Brown teaches a graduate course in bioinformatics at the Sackler Institute of the NYU School of Medicine and is a faculty member in the NYU Structural Biology Training Program. Dr. Brown serves as a member of the Scientific Advisory Boards for bioinformatics and genomics and is chair of the microarray database committee for the Academic Medical Development Consortium(AMDeC). Contact InformationJudith D. Goldberg, Sc.D. ProfessorDepartment of Environmental Medicine New York University School of Medicine 650 1st Avenue, 5th Floor New York, NY 10016 Phone: (212)263-0314 FAX: (212)263-8570 E-mail: jd.goldberg@.med.nyu.eduServices AvailableBiostatistics and bioinformatics collaboration and support for peer-reviewed, funded projects, and the development of new research initiatives and pilot studies including: Study planning including study design and analysis planning Study implementation and conduct including data management consultation Statistical Analysis Mathematical modeling Interpretation and reporting of results Bioinformatics support Operational PoliciesThe resources of the Environmental Health Statistics and Bioinformatics Facility Core are available to all members of the NIEHS Center. The policy of the Facility Core is to provide statistical collaboration and bioinformatics support to all investigators in the Research Cores and Facility Cores for the development of new environmental health research initiatives, for the design and analysis of pilot studies to develop new research initiatives, and for short-term and unanticipated research projects that are part of funded research projects. Special emphasis is placed on supporting collaborations among research cores. The Director of the Facility Core ensures the efficient and effective functioning of the resource. The assignment of Facility Core members to particular projects or investigators is based primarily on the project requirements and availability of appropriate staff expertise. The Facility Core sets its priorities in coordination with the Center’s Internal Advisory Committee of which the Director is a member. Experimental Animal Services Facility Core Maarten Bosland, D.V.Sc., Ph.D. Professor of Environmental Medicine and Urology Education: Dr. Bosland received his Ph.D. in 1989 from Utrecht University (The Netherlands) and his D.V.Sc. in 1978 from Utrecht University (The Netherlands). Postdoctoral Training: Dr. Bosland received Post-doctoral training in Human Pathology at the Antonius Hospital, Utrecht, The Netherlands, in Toxicologic Pathology at TNO-Toxicology & Nutrition, Zeist, The Netherlands, and in Laboratory Animal Science at Utrecht University. He is board certified in Laboratory Animal Science and in Laboratory Animal Pathology (from The Netherlands). Other Responsibilities: Director of the Experimental Animal Service Facility Core of the NYU Environmental Health Sciences Center of Excellence; Director of the Histopathology & Experimental Animal Facility shared resources of the NYU Cancer Institute; Program Co-Leader of the Genitourinary Cancer Program of the NYU Cancer InstituteResearch Interests: Prostate cancer; Cancer chemoprevention; Hormonal carcinogenesis; Breast cancer; Endocrine disruption; Experimental pathology Contact: E-mail: maarten.bosland@med.nyu.edu TEL: (845) 731-3583 and (212) 263-0333 FAX: (845) 351-3492 NYU NIEHS Center Experimental Animal Services Facility Core Purpose The objectives of the facility core are to provide the following array of integrated services that are driven by the scientific needs of members of the NIEHS Center:
Personnel Maarten C. Bosland, D.V.Sc., Ph.D., Director: over 17 years of experience in experimental pathology and animal facility management; board certified in Laboratory Animal Science and Laboratory Animal Pathology (the Netherlands). He has direct responsibility for the Histopathology and the Experimental Animal Units and for the health surveillance program of the Animal Facility and for comparative pathology services. Lung Chi Chen, Ph.D., Co-Director of the Experimental Animal Facility Core for the Inhalation Exposure Unit: over 2 decades of experience in inhalation toxicology including the generation and characterization of exposure atmospheres. Toya McWilliams, D.V.M., Director of NYU Medical Center's Division of Laboratory Animal Science and of its Central Animal Facility: attending veterinarian with responsibility for the veterinary oversight of the Department of Environmental Satellite Animal Facility, particularly for providing clinical veterinary services. Katherine Seymour, AALAS-certified Laboratory Animal Technologist, the Experimental Animal Unit Supervisor: over 35 years of experience in laboratory animal research, over 15 years as supervisory technician at this Center. Linda Ayres, AALAS-certified Laboratory Animal Technologist, Deputy Experimental Animal Unit Supervisor: has special responsibilities for assuring safe working practices and personal protection as the Institute of Environmental Medicine's Safety Officer. Robert Kelly, Experimental Animal Unit Technician, AALAS-certified: the primary technician involved in specialized animal care activities serving the needs of NIEHS Center memberswith over 25 years experience. Michael Barbieri, AALAS-certified Laboratory Animal Technician, Experimental Animal Unit Technicianwith partial responsibility for the inhalation exposures of this unit. Kenneth Magar, supervisory technician of the Inhalation Exposure Unit, provides technical assistance and oversight: 38 years experience in the conduct of inhalation exposures at NYU and responsible for animal exposures, test atmosphere generation, chamber maintenance, and calibration and maintenance of air sampling. Sally Lasano, Histopathology Supervisory Technician. Contact InformationMaarten Bosland, D.V.Sc., Ph.D. Professor Department of Environmental Medicine New York University School of Medicine 57 Old Forge Road Tuxedo, NY 10987 Phone: (845) 731-3583 and (212) 263-0333 FAX: (845) 351-3492 E-mail: maarten.bosland@med.nyu.edu Services Available Experimental Animal Unit: The Experimental Animal Unit provides uniform dosing and specialized housing for the animals used in the extensive and diverse environmental health studies by Center members. The Experimental Animal Unit is a formal Satellite of the NYUSOM's AAALAC-accredited Central Animal Facility and Division of Laboratory Animal Science, which provides veterinary care supervision for the Unit. The Unit includes several animal biosafety level (ABSL)-2 and carcinogen containment rooms, and facilities for research with immunocompromised mice as well as for necropsies and various types of treatments typically used in animal experimentation. Inhalation Exposure Unit: The Inhalation Exposure Unit furnishes the specialized equipment necessary to perform inhalation exposure studies and provides personnel trained in generation of test atmospheres containing particles and gases and dosing of animals with these atmospheres. Development and optimization of new inhalation procedures and set up of new studies are provided at no cost. The Inhalation Exposure Unit includes:
Histopathology Unit The Histopathology Unit offers rapid turn-around of routine as well as non-routine services according to the needs of NIEHS Center members. New methods or potentially difficult techniques such as immunochemistry can be set up and optimized at no cost. The Histopathology Unit includes:
Operational PoliciesPrioritization is on a first-come-first-serve basis and Center members can "make reservations" ahead of time. However, pilot projects that are intended to develop preliminary results for grant applications are given special priority if at all possible. If prioritization conflicts arise, projects of Center members are given priority over projects of non-members and peer-reviewed projects are given priority over non-peer-reviewed projects. The Facility Core has a Users committee whose role is to review the current and future activities of the Core, to identify changes in needs and use that are anticipated, to provide advice to the Core Director and Co-Director, and to discuss any prioritization conflicts that may have arisen. Molecular
and Cell Biology Education: Dr. Klein received her Ph.D. from New York University in 1988. Postdoctoral Training: Dr. Klein did her postdoctoral training at New York University with Dr. Costa and Dr. Snow. Other Responsibilities: Dr. Klein is the Director of Facility Cores for the NIEHS Center; and is Director of the Molecular and Cell Biology Facility Core of the NIEHS Center. She also provides experimental non-clinical cytogenetics consultation and technical expertise to the NYU Cancer Center. Dr. Klein has served as a grant reviewer for the NIEHS HBCU Program Projects, the NCI on a Special Emphasis Panel and the EPA (Biochemistry, Molecular Biology and Genetics Fellowships). She is a member of the American Society of Human Genetics (ASHG), the American Society for Cancer Research (AACR), the American Society for the Advancement of Science (AAAS) and the Environmental Mutagen Society (EMS) for which she serves on the Nominations Committee (2004) and the 2005 International EMS Meeting Program Committee. Research Interests: Dr. Klein is a member of the Molecular Toxicology and Carcinogenesis Research Core of the NIEHS Center. Her research interests include the study of epigenetic environmental carcinogens, transgenic mammalian mutagenesis models, molecular cytogenetics and the investigation of a variety of dietary antioxidants that act as antimutagens or anticarcinogens, perhaps via modulation of epigenetic processes. Contact: E-Mail: kleinc@env.med.nyu.edu TEL: (845) 731-3510 FAX: (845) 351-2058 Molecular and Cell Biology Facility Core NYU NIEHS Center Molecular and Cell Biology Facility Core PurposeResearch in the area of environmental health sciences requires multidisciplinary approaches, utilization of new, accurate and highly sensitive molecular techniques and the use of state-of-the-art instruments that allow researchers to obtain, store, quantitate, and organize experimental molecular biology data. Since cutting-edge, high-throughput instruments are expensive and not affordable to individual investigators, the Molecular and Cell Biology Facility Core maintains and upgrades this essential dedicated equipment for shared use by molecular biology researchers. Also, since the most current molecular approaches require considerable training before their routine implementation, this Facility Core provides expert instruction and consultation services on the use of these techniques. In order to maintain cutting-edge status, the Facility Core continues to acquire new state-of-the-art, high cost equipment through competitive and institutional funding sources. To further support the molecular biology research in the Center, the Molecular and Cell Biology Facility Core operates an on-site commercial store with well-stocked freezers containing a wide variety of discounted restriction and modifying enzymes, and DNA sequencing and PCR reagents and kits. Since many Center researchers who are active in the area of molecular biology use cultured cells in their research, this Facility Core also provides highly discounted tissue culture media and fetal bovine serum at considerable cost savings compared to commercial suppliers. The Facility Core offers consultation regarding preparation of specialized tissue culture media, and identification of less expensive or alternate media or serum substitutes. Personnel Catherine B. Klein, Ph.D., Director, has over 25 years experience in mammalian cell culture and molecular biology. As Facility Core Director, she interacts with funded investigators to coordinate the efficient use of all available services. Dr. Klein assumes overall responsibility for the operations of the entire facility and in that capacity is the direct supervisor of its technical staff. For the molecular biology supplies center and the Media Preparation Center, Dr. Klein provides quality control and sterility assurances. Isaac Wirgin, Ph.D.,Co-Director, is an experienced molecular/population biologist (17 years at NYU) with research interests in transcriptional regulation of gene expression in toxicant-exposed cells and organisms and population genetics. Dr. Wirgin has a long history of identifying intrinsic and extrinsic factors that impact gene expression at the mRNA level. Dr. Wirgin is responsible for overseeing the daily operations of all molecular biology equipment within the Core, including technical support, interactions with companies for contractual negotiations, as well as maintenance and service of all instruments. Dr. Nirmal Roy, Ph.D., is the Molecular Biology Consultant for the Molecular and Cell Biology Facility Core. He is a Research Assistant Professor of Environmental Medicine with more than 20 years of experience in molecular and cellular biology including the cloning of various toxicological relevant genes from fish models (cytochrome P450s and AHR pathway molecules). Dr. Roy advises on the use and provides the appropriate negative, positive, and normalization controls for all hybridizations. He assists in the evaluation of the printing success including analyses of spot morphometry, hybridization potential of probe, and evaluation of background on all slides. He also advises on use of the scanner to obtain, evaluate and initially organize hybridization data. Dr. Roy is responsible for advising new users on sequencing on the automated DNA sequencer, and he responsible for the preparation and labeling of DNA targets to be used on Affymetrix GeneChip and custom microarrays. Lorraine Maceda, is an experienced molecular biology technician for the past 15 years and serves as Molecular Biology Technician for the Facility Core. Ms. Maceda has many years of experience in genotyping and has optimized use of the DNA sequencer for DNA fragment/microsatellite analysis. Ms. Maceda, under the supervision of Co-Director Dr. Wirgin, provides on-site technical advice to research investigators, maintains contacts with service people, and is responsible for the routine weekly maintenance of the PhosphorImager, fluorescent microplate reader, Alpha Innotech Imager, Kodak developer, Kodak camera, DNA sequencer, and microarray spotter/scanner. Linda Tomlinson, is the Facility Core technician responsible for all dishwashing services related either to media preparation or lab maintenance, the weekly maintenance of the Millipore water systems, the ordering of supplies and ingredients required to provide all these services, the daily scheduling of autoclave use, and oversight of the operations and maintenance of the autoclave and dishwashing equipment. She has over ten years experience at NYU performing these duties. Contact InformationCatherine B. Klein, Ph.D.Assistant Professor Department of Environmental Medicine New York University School of Medicine 57 Old Forge Road Tuxedo, NY 10987 Phone: (845) 731-3510 Fax: (845) 351-2058 E-mail: kleinc@env.med.nyu.edu Services Available The Molecular and Cell Biology Facility Core provides Center investigators with:
2) consultation and hands-on assistance in the isolation of cDNAs, design of microarrays, printing of probes on custom array slides, hybridizations, evaluation of hybridization success, and acquisition and initial organization of data from commercial and custom microarrays. The Core will also prepare labeled targets for custom microarray and Affymetrix GeneChip hybridizations. The Molecular and Cell Biology Facility Core will work closely with the Environmental Health Statistics and Bioinformatics Facility Core in the design of microarray experiments and in the analysis of their data. 3) technical support and investigator training for the use of shared molecular biology imaging instruments including a PhosphorImager, Fluorescent Microplate reader, AlphaImager, and Kodak film developer. The Facility Core also helps to develop new methods employing this equipment. 4) consultation and training on commonly used molecular biology techniques including, but not limited to, Northern, Southern, and Western blots, real-time PCR, RT-PCR, and gel mobility shift analyses. This Core will also aid Center investigators in use of software available in the Genetics Computer Group (GCG) package. 5) basic molecular and cell biology research and laboratory support including (a) an "on-site" commercial supply of discounted molecular biology enzymes and reagents, (b) freshly prepared, highly discounted tissue culture media, buffers, and serum, and (c) cost efficient MilliQ water, dishwashing, and autoclaving services. Operational PoliciesThe operational policies of the Molecular and Cell Biology Facility Core are as follows: The Imaging Center is open seven days a week, and is available to all investigators who have completed training and do not need supervision. Qualified users include any investigator who is entitled to receive research funding via NYUSOM. Those individuals requiring assistance can be assisted by the Facility Core technician, according to the schedule posted in the Imaging Center. Investigators reserve a block of time (30 min- 4 hr) in advance, recorded in a logbook for the use of each instrument. Each new investigator will have their own password, and a computer program will automatically calculate individual investigator use for our records. All Center members have access to the Laboratory Supplies Unit on a first come, first served basis, during usual working hours (8AM to 5 PM, M-F). All operations of the Genotyping, Microarray, and Imaging Units of the Facility Core are carried out by personnel with over 20 years experience in molecular biology according to detailed protocols established by the facility Co-Director Dr. Wirgin and Molecular Consultant, Dr. Roy, after consultation with the individual investigators. Written protocols are available to all investigators upon request and on the Web. A user committee comprised of several active molecular biology researchers including Drs. I. Wirgin, C. Klein, T. Gordon, C. Huang, N. Roy, M.-s. Tang and R. Shore reviews the daily operations and future directions of this Facility Core.
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