Mamta V Tahiliani

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Mamta V Tahiliani, PhD

Assistant Professor;
Department of Biochemistry and Molecular Pharmacology and Administration (Administration)

Contact Info

Address
540 First Ave
Floor 4 Room lab ofc4
Skirball Institute
New York, NY 10016

212-263-2854
Mamta.Tahiliani@med.nyu.edu


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Research Summary

5-methylcytosine is a minor base in mammalian DNA, which plays a disproportionately critical role in development. DNA methylation is very dynamic during embryogenesis and is vital for parental imprinting, X inactivation, silencing of endogenous retroviruses as well as the regulation of genomic stability. De novo methylation and demethylation also occur in somatic cells during differentiation, tumorigenesis and aging. In contrast to the established relation between DNA methylation, DNA methyltransferases and gene silencing, the enzymes and processes regulating DNA demethylation are not well understood. We have recently discovered that TET1 catalyzes the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). We also found that hmC can be detected in the genome of mouse ES cells, and that both TET1 levels and hmC levels decline when ES cells are differentiated. These results suggest that hmC is a normal constituent of mammalian DNA, and identify TET1 as an enzyme with a potential role in epigenetic regulation through modification of 5mC. This study described a new class of enzymes that catalyze a new modification of DNA and alters our perception of how DNA methylation status may be regulated in cells. The description of the regulated conversion of 5mC to hmC raises an enormous number of new questions that are of pressing importance. The goal of my laboratory is to integrate hmC into known pathways of 5mC metabolism and to determine how hmC exerts its influence on the genome with the ultimate goal of understanding the role that 5mC and hmC play in genomic stability.

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All data from NYU Health Sciences Library Faculty Bibliography — -

Contact:
http://hsl.med.nyu.edu/faculty-bibliography-search

Simultaneous sequencing of oxidized methylcytosines produced by TET/JBP dioxygenases in Coprinopsis cinerea
Chavez, Lukas; Huang, Yun; Luong, Khai; Agarwal, Suneet; Iyer, Lakshminarayan M; Pastor, William A; Hench, Virginia K; Frazier-Bowers, Sylvia A; Korol, Evgenia; Liu, Shuo; Tahiliani, Mamta; Wang, Yinsheng; Clark, Tyson A; Korlach, Jonas; Pukkila, Patricia J; Aravind, L; Rao, Anjana
2014 Dec;111(48):E5149-E5158, Proceedings of the National Academy of Sciences of the United States of America
— id: 1369032, year: 2014, vol: 111, page: E5149, stat: Journal Article,

Expanding the epigenetic landscape: novel modifications of cytosine in genomic DNA
Kriaucionis, Skirmantas; Tahiliani, Mamta
2014 Oct;6(10):a018630-a018630 a018630, Cold Spring Harbor perspectives in biology
— id: 1360302, year: 2014, vol: 6, page: a018630, stat: Journal Article,

Tet1 and Tet2 regulate 5-hydroxymethylcytosine production and cell lineage specification in mouse embryonic stem cells
Koh, Kian Peng; Yabuuchi, Akiko; Rao, Sridhar; Huang, Yun; Cunniff, Kerrianne; Nardone, Julie; Laiho, Asta; Tahiliani, Mamta; Sommer, Cesar A; Mostoslavsky, Gustavo; Lahesmaa, Riitta; Orkin, Stuart H; Rodig, Scott J; Daley, George Q; Rao, Anjana
2011 Feb;8(2):200-213, Cell stem cell
— id: 232452, year: 2011, vol: 8, page: 200, stat: Journal Article,

Genome-wide mapping of 5-hydroxymethylcytosine in embryonic stem cells
Pastor, William A; Pape, Utz J; Huang, Yun; Henderson, Hope R; Lister, Ryan; Ko, Myunggon; McLoughlin, Erin M; Brudno, Yevgeny; Mahapatra, Sahasransu; Kapranov, Philipp; Tahiliani, Mamta; Daley, George Q; Liu, X Shirley; Ecker, Joseph R; Milos, Patrice M; Agarwal, Suneet; Rao, Anjana
2011 May;473(7347):394-397, Nature
— id: 232442, year: 2011, vol: 473, page: 394, stat: Journal Article,

The behaviour of 5-hydroxymethylcytosine in bisulfite sequencing
Huang, Yun; Pastor, William A; Shen, Yinghua; Tahiliani, Mamta; Liu, David R; Rao, Anjana
2010 ;5(1):e8888-e8888, PLoS one
— id: 116726, year: 2010, vol: 5, page: e8888, stat: Journal Article,

Impaired hydroxylation of 5-methylcytosine in myeloid cancers with mutant TET2
Ko, Myunggon; Huang, Yun; Jankowska, Anna M; Pape, Utz J; Tahiliani, Mamta; Bandukwala, Hozefa S; An, Jungeun; Lamperti, Edward D; Koh, Kian Peng; Ganetzky, Rebecca; Liu, X Shirley; Aravind, L; Agarwal, Suneet; Maciejewski, Jaroslaw P; Rao, Anjana
2010 Dec 9;468(7325):839-843, Nature
— id: 116732, year: 2010, vol: 468, page: 839, stat: Journal Article,

Prediction of novel families of enzymes involved in oxidative and other complex modifications of bases in nucleic acids
Iyer, Lakshminarayan M; Tahiliani, Mamta; Rao, Anjana; Aravind, L
2009 Jun 1;8(11):1698-1710, Cell cycle
— id: 116724, year: 2009, vol: 8, page: 1698, stat: Journal Article,

Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1
Tahiliani, Mamta; Koh, Kian Peng; Shen, Yinghua; Pastor, William A; Bandukwala, Hozefa; Brudno, Yevgeny; Agarwal, Suneet; Iyer, Lakshminarayan M; Liu, David R; Aravind, L; Rao, Anjana
2009 May 15;324(5929):930-935, Science
— id: 116723, year: 2009, vol: 324, page: 930, stat: Journal Article,

Mouse Eri1 interacts with the ribosome and catalyzes 5.8S rRNA processing
Ansel, K Mark; Pastor, William A; Rath, Nicola; Lapan, Ariya D; Glasmacher, Elke; Wolf, Christine; Smith, Laura C; Papadopoulou, Nikoletta; Lamperti, Edward D; Tahiliani, Mamta; Ellwart, Joachim W; Shi, Yujiang; Kremmer, Elisabeth; Rao, Anjana; Heissmeyer, Vigo
2008 May;15(5):523-530, Nature structural & molecular biology
— id: 116720, year: 2008, vol: 15, page: 523, stat: Journal Article,

The histone H3K4 demethylase SMCX links REST target genes to X-linked mental retardation
Tahiliani, Mamta; Mei, Pinchao; Fang, Rui; Leonor, Thiago; Rutenberg, Michael; Shimizu, Fumiko; Li, Jing; Rao, Anjana; Shi, Yujiang
2007 May 31;447(7144):601-605, Nature
— id: 116716, year: 2007, vol: 447, page: 601, stat: Journal Article,

PtdIns(4,5)P2 functions at the cleavage furrow during cytokinesis
Field, Seth J; Madson, Nikki; Kerr, Monica L; Galbraith, Kenneth A A; Kennedy, Caitlin E; Tahiliani, Mamta; Wilkins, Andrew; Cantley, Lewis C
2005 Aug 9;15(15):1407-1412, Current biology. CB
— id: 116709, year: 2005, vol: 15, page: 1407, stat: Journal Article,

Concerted dephosphorylation of the transcription factor NFAT1 induces a conformational switch that regulates transcriptional activity
Okamura, H; Aramburu, J; Garcia-Rodriguez, C; Viola, J P; Raghavan, A; Tahiliani, M; Zhang, X; Qin, J; Hogan, P G; Rao, A
2000 Sep;6(3):539-550, Molecular cell
— id: 116701, year: 2000, vol: 6, page: 539, stat: Journal Article,