Matthias Stadtfeld

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Matthias Stadtfeld

Assistant Professor, Department of Cell Biology

Contact Info

Matthias.Stadtfeld@med.nyu.edu


Research Summary

Pluripotent cells that have the unique ability to form all cell types of the adult body can be derived in two different ways: 1) by explanting early mammalian embryos, thereby giving rise to embryonic stem (ES) cells and 2) by the enforced expression of defined embryonic transcription factors in adult somatic cells, giving rise to induced pluripotent stem (iPS) cells. The latter process is commonly referred to as reprogramming and allows for the comparatively straightforward generation of patient-specific pluripotent stem cells to study, and ultimately possibly treat, degenerative disorders. In addition, iPSC technology represents a tractable experimental approach to study mammalian development. Research in my laboratory uses reprogramming technology to identify mechanisms that control gene expression and determine cellular identity, using the mouse as the main model organism. One major focus of research will be determining the reasons for the occurrence of epigenetic abnormalities that are frequently introduced in specific genomic regions during iPS cell formation. These abnormalities limit the developmental potential of iPS cells and their study will help unraveling the molecular requirements for faithful epigenetic reprogramming. A second major goal will be using pluripotent cells for the in vitro generation of adult-type stem cells that are functionally equivalent to their in vivo counterparts found in the body. We are especially interested in understanding the molecular determinants of blood cell specification and, ultimately, the generation of functional hematopoietic stem cells from ES cells and iPS cells.

Co-repressor CBFA2T2 regulates pluripotency and germline development
Tu, Shengjiang; Narendra, Varun; Yamaji, Masashi; Vidal, Simon E; Rojas, Luis Alejandro; Wang, Xiaoshi; Kim, Sang Yong; Garcia, Benjamin A; Tuschl, Thomas; Stadtfeld, Matthias; Reinberg, Danny. Co-repressor CBFA2T2 regulates pluripotency and germline development. Nature. 2016 Jun 8;534(7607):387-390 (2136522)

The histone chaperone CAF-1 safeguards somatic cell identity
Cheloufi, Sihem; Elling, Ulrich; Hopfgartner, Barbara; Jung, Youngsook L; Murn, Jernej; Ninova, Maria; Hubmann, Maria; Badeaux, Aimee I; Euong Ang, Cheen; Tenen, Danielle; Wesche, Daniel J; Abazova, Nadezhda; Hogue, Max; Tasdemir, Nilgun; Brumbaugh, Justin; Rathert, Philipp; Jude, Julian; Ferrari, Francesco; Blanco, Andres; Fellner, Michaela; Wenzel, Daniel; Zinner, Marietta; Vidal, Simon E; Bell, Oliver; Stadtfeld, Matthias; Chang, Howard Y; Almouzni, Genevieve; Lowe, Scott W; Rinn, John; Wernig, Marius; Aravin, Alexei; Shi, Yang; Park, Peter J; Penninger, Josef M; Zuber, Johannes; Hochedlinger, Konrad. The histone chaperone CAF-1 safeguards somatic cell identity. Nature. 2015 Dec 10;528(7581):218-224 (1877752)

F-class cells: new routes and destinations for induced pluripotency
Vidal, Simon E; Stadtfeld, Matthias; Apostolou, Eftychia. F-class cells: new routes and destinations for induced pluripotency. Cell stem cell. 2015 Jan 8;16(1):9-10 (1435902)

Combinatorial Modulation of Signaling Pathways Reveals Cell-Type-Specific Requirements for Highly Efficient and Synchronous iPSC Reprogramming
Vidal, Simon E; Amlani, Bhishma; Chen, Taotao; Tsirigos, Aristotelis; Stadtfeld, Matthias. Combinatorial Modulation of Signaling Pathways Reveals Cell-Type-Specific Requirements for Highly Efficient and Synchronous iPSC Reprogramming. Stem cell reports. 2014 Oct 14;3(4):574-584 (1323032)

Nucleosomal occupancy changes locally over key regulatory regions during cell differentiation and reprogramming
West, Jason A; Cook, April; Alver, Burak H; Stadtfeld, Matthias; Deaton, Aimee M; Hochedlinger, Konrad; Park, Peter J; Tolstorukov, Michael Y; Kingston, Robert E. Nucleosomal occupancy changes locally over key regulatory regions during cell differentiation and reprogramming. Nature communications. 2014 Aug 27;5:4719-4719 (1161652)