Matthias Stadtfeld

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Matthias Stadtfeld

Assistant Professor, Department of Cell Biology

Contact Info

Matthias.Stadtfeld@med.nyu.edu


Research Summary

Pluripotent cells that have the unique ability to form all cell types of the adult body can be derived in two different ways: 1) by explanting early mammalian embryos, thereby giving rise to embryonic stem (ES) cells and 2) by the enforced expression of defined embryonic transcription factors in adult somatic cells, giving rise to induced pluripotent stem (iPS) cells. The latter process is commonly referred to as reprogramming and allows for the comparatively straightforward generation of patient-specific pluripotent stem cells to study, and ultimately possibly treat, degenerative disorders. In addition, iPSC technology represents a tractable experimental approach to study mammalian development. Research in my laboratory uses reprogramming technology to identify mechanisms that control gene expression and determine cellular identity, using the mouse as the main model organism. One major focus of research will be determining the reasons for the occurrence of epigenetic abnormalities that are frequently introduced in specific genomic regions during iPS cell formation. These abnormalities limit the developmental potential of iPS cells and their study will help unraveling the molecular requirements for faithful epigenetic reprogramming. A second major goal will be using pluripotent cells for the in vitro generation of adult-type stem cells that are functionally equivalent to their in vivo counterparts found in the body. We are especially interested in understanding the molecular determinants of blood cell specification and, ultimately, the generation of functional hematopoietic stem cells from ES cells and iPS cells.

Research Keywords

pluripotent stem cells, cellular reprogramming, stem cell biology, epigenetic mechanisms, hematopoiesis

Probabilistic Modeling of Reprogramming to Induced Pluripotent Stem Cells
Liu, Lin L; Brumbaugh, Justin; Bar-Nur, Ori; Smith, Zachary; Stadtfeld, Matthias; Meissner, Alexander; Hochedlinger, Konrad; Michor, Franziska. Probabilistic Modeling of Reprogramming to Induced Pluripotent Stem Cells. Cell reports. 2016 Dec 20;17(12):3395-3406 (2374582)

A reporter model to visualize imprinting stability at the Dlk1 locus during mouse development and in pluripotent cells
Swanzey, Emily; Stadtfeld, Matthias. A reporter model to visualize imprinting stability at the Dlk1 locus during mouse development and in pluripotent cells. Development. 2016 Nov 15;143(22):4161-4166 (2278682)

Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a
Strikoudis, Alexandros; Lazaris, Charalampos; Trimarchi, Thomas; Galvao Neto, Antonio L; Yang, Yan; Ntziachristos, Panagiotis; Rothbart, Scott; Buckley, Shannon; Dolgalev, Igor; Stadtfeld, Matthias; Strahl, Brian D; Dynlacht, Brian D; Tsirigos, Aristotelis; Aifantis, Iannis. Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a. Nature cell biology. 2016 Nov;18(11):1127-1138 (2279842)

Co-repressor CBFA2T2 regulates pluripotency and germline development
Tu, Shengjiang; Narendra, Varun; Yamaji, Masashi; Vidal, Simon E; Rojas, Luis Alejandro; Wang, Xiaoshi; Kim, Sang Yong; Garcia, Benjamin A; Tuschl, Thomas; Stadtfeld, Matthias; Reinberg, Danny. Co-repressor CBFA2T2 regulates pluripotency and germline development. Nature. 2016 Jun 08;534(7607):387-390 (2136522)

The histone chaperone CAF-1 safeguards somatic cell identity
Cheloufi, Sihem; Elling, Ulrich; Hopfgartner, Barbara; Jung, Youngsook L; Murn, Jernej; Ninova, Maria; Hubmann, Maria; Badeaux, Aimee I; Euong Ang, Cheen; Tenen, Danielle; Wesche, Daniel J; Abazova, Nadezhda; Hogue, Max; Tasdemir, Nilgun; Brumbaugh, Justin; Rathert, Philipp; Jude, Julian; Ferrari, Francesco; Blanco, Andres; Fellner, Michaela; Wenzel, Daniel; Zinner, Marietta; Vidal, Simon E; Bell, Oliver; Stadtfeld, Matthias; Chang, Howard Y; Almouzni, Genevieve; Lowe, Scott W; Rinn, John; Wernig, Marius; Aravin, Alexei; Shi, Yang; Park, Peter J; Penninger, Josef M; Zuber, Johannes; Hochedlinger, Konrad. The histone chaperone CAF-1 safeguards somatic cell identity. Nature. 2015 Dec 10;528(7581):218-224 (1877752)