Biosketch / Results /
David R Schwartz, M.D.Associate Professor;
Department of Medicine (Pulmy&CCM Div)
NYU Critical Care Associates
Clinical Addresses530 FIRST AVENUE
NEW YORK, NY 10016
Medical SpecialtiesPulmonary Medicine, Critical Care Medicine, Internal Medicine
Medical ExpertiseLung Cancer
InsuranceEBCBS CHLD HLTH, EBCBS EPO, EBCBS HLTHY NY, EBCBS HMO, EBCBS INDEMNITY, EBCBS MEDIBLUE, EBCBS POS, EBCBS PPO, Medicare, NY MEDICAID, Railroad Medicare, Tricare, UHC TOP TIER
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Board Certification1998 — Ab Internal Medicine (Pulmonary Disease)
1999 — Ab Internal Medicine (Critical Care Medicine)
Education1992 — SUNY Health Sciences Center at Brooklyn - Downstate Medical, Medical Education
1992-1996 — NYU Medical Center (Internal Medicine), Residency Training
1996-2000 — Massachusetts General Hospital (Pulmonary & Critical Care Medicine), Clinical Fellowships
Research SummaryOur laboratory's broad, long-term objective is to develop and apply new single-molecule methodologies to ultra-rapid, high resolution human and microorganism genomic analysis. To improve mapping rates and resolution, we developed Optical Mapping, a powerful non-electrophoretic approach that rapidly creates high-resolution ordered restriction maps from a variety of clone-types and genomic DNA. Optical mapping uses fluorescence microscopy imaging techniques, coupled with sophisticated statistical analysis, to determine restriction fragment order and size generated by enzymatic action on individual DNA molecules. Additionally, novel sequence analysis approaches are being developed around the concept of imaging polymerase action on single molecule templates.
Robotic "ridding" techniques, combined with novel molecular fixation techniques permit the biochemical action and evaluation of a large number of samples in parallel. Given the ability to grid multiple samples, and assay biochemistries on the single molecule level, we have developed an integrated system to robotically deposit samples, and image substrate molecules using automated fluorescence microscopy. To complement these automation advancements, we have developed new approaches for ordered restriction map construction using Bayesian inference techniques. These map construction algorithms work with maps automatically derived from single molecules, and are notably, the first practical approaches for the automated analysis of data derived from large populations of single molecules. Considered together, these advances constitute an integrated mapping system which eliminates operator interaction with the system from molecular deposition through map construction. The full utility of this system is derived ranging from the synthesis of new single molecule fixation effects, biochemistries and statistical analysis approaches, and should prove to be universally applicable to the analysis other single molecule systems.
High resolution mapping has enormous utility in the genomic analysis of mammalian genomes and microorganisms. Optical mapping is currently playing a major role in the international effort to sequence human genome by providing rigorous scaffolds for the assembly and verification of large scale sequencing efforts. Other applications in the laboratory are centered on the direct mapping of genomic DNA extracted from bacteria and pathogenic parasites-bypassing the need for libraries, with the goal of analyzing large number of strains to develop new approaches to the functional analysis of genomes.