Manijeh Mohraz, Ph.D.

Research Associate Professor; Instructor-Histology
Department of Cell Biology (Cell Biology)

Contact Info

Address
550 First Avenue
Floor 6 Room 627
Medical Science Building
New York, NY 10016

Phone: 212-263-5357
Email: manijeh.mohraz@med.nyu.edu

« Back to Results

Research Summary

on-transporting ATPases, or "ion pumps," couple ATP hydrolysis to active ion translocation across membranes, a function essential for cell viability. Although much is understood about ion pumps, details of their transport mechanisms are unknown. Of utmost importance is delineating the three-dimensional structure at high resolution, which would allow integration of the biochemical and kinetic information. We focus on elucidating the three-dimensional structure of Na,K-ATPase, the Na+,K+ pump in the plasma membrane of all animal cells, using electron microscopy of two-dimensional crystals and image reconstruction from the recorded micrographs, complemented with relevant biochemical investigations.

We obtained the three-dimensional structure of Na,K-ATPase at 2.5-nm resolution and mapped the locations of its two subunits. Moreover, results from the combined structural and biochemical studies have implications for the oligomeric form of the functional unit and how ion transport might occur. Based on these findings, we proposed a model for the form and mechanism of the Na+,K+ pump.

Our continuing goals are to extend the structural resolution to 1.0 nm, which would allow identifying structures such as helices and channels, and pursue structural studies on H,K-ATPase, the stomach acid pump.

Research Interests

Structural Studies of Ion-transporting ATPases

Research Keywords

electron microscopy, image reconstruction, ion-transporting ATPases, membrane protein, Na+,K+ pump, two-dimensional crystals

« Back to Results

All data from NYU Health Sciences Library Faculty Bibliography — -

Contact:
http://hsl.med.nyu.edu/faculty-bibliography-search#about

Reconstitution of detergent-solubilized Na,K-ATPase and formation of two-dimensional crystals
Mohraz M
1999 Mar;125(1):76-85, Journal of structural biology
— id: 8513, year: 1999, vol: 125, page: 76, stat: Journal Article,

Reconstitution and crystallization of solubilized Na,K-ATPase
Mohraz, M
1999 JAN ;76(1):A451-A451, Biophysical journal
— id: 54000, year: 1999, vol: 76, page: A451, stat: Journal Article,

Production of recombinant Na1K-ATPase for structural studies
Mohraz, M; Morimoto, T
1999 NOV ;10(5):80A-80A, Molecular biology of the cell
— id: 53777, year: 1999, vol: 10, page: 80A, stat: Journal Article,

Reconstitution and crystallization of solubilized Na,K-ATPase
Mohraz, M
1997 NOV ;8(5):484-484, Molecular biology of the cell
— id: 53161, year: 1997, vol: 8, page: 484, stat: Journal Article,

Immunoelectron microscopy of epitopes on Na,K-ATPase catalytic subunit. Implications for the transmembrane organization of the C-terminal domain
Mohraz M; Arystarkhova E; Sweadner KJ
1994 Jan 28;269(4):2929-2936, Journal of biological chemistry
— id: 8430, year: 1994, vol: 269, page: 2929, stat: Journal Article,

MEMBRANE TOPOLOGY OF THE ALPHA SUBUNIT OF NA,K-ATPASE
MOHRAZ, M; ARYSTARKHOVA, E; SWEADNER, KJ
1993 FEB ;64(2):A331-A331, Biophysical journal
— id: 54341, year: 1993, vol: 64, page: A331, stat: Journal Article,

STRUCTURAL STUDY OF H,K-ATPASE BY ELECTRON-MICROSCOPY AND IMAGE-PROCESSING
Mohraz, M; Sathe, S; Smith, PR
1990 Feb;57(2):A357-A357, Biophysical journal
— id: 32111, year: 1990, vol: 57, page: A357, stat: Journal Article,

Three-dimensional structure of Na,K-ATPase and a model for the oligomeric form and the mechanism of the Na,K pump
Mohraz M; Smith PR
1988 ;268A:99-106, Progress in clinical & biological research
— id: 11284, year: 1988, vol: 268A, page: 99, stat: Journal Article,

The three-dimensional structure of the Na,K-ATPase from electron microscopy
Mohraz M; Simpson MV; Smith PR
1987 Jul;105(1):1-8, Journal of cell biology
— id: 8309, year: 1987, vol: 105, page: 1, stat: Journal Article,

3-D STRUCTURE OF NA,K-ATPASE BY ELECTRON-MICROSCOPY AND IMAGE- RECONSTRUCTION
Mohraz, M; Smith, PR
1987 Feb;51(2):A174-A174, Biophysical journal
— id: 31413, year: 1987, vol: 51, page: A174, stat: Journal Article,

The structure of the adenovirus capsid. III. Hexon packing determined from electron micrographs of capsid fragments
van Oostrum, J; Smith, P R; Mohraz, M; Burnett, R M
1987 Nov 5;198(1):73-89, Journal of molecular biology
— id: 150636, year: 1987, vol: 198, page: 73, stat: Journal Article,

THE VERTEX MORPHOLOGY OF THE ADENOVIRUS CAPSID
Vanoostrum, J; Smith, PR; Mohraz, M; Burnett, RM
1987 May 27;494(11):423-426, Annals of the New York Academy of Sciences
— id: 31309, year: 1987, vol: 494, page: 423, stat: Journal Article,

Structural studies of Na,K-ATPase
Mohraz, M; Yee, M; Smith, P R
1986 ;483:131-139, Annals of the New York Academy of Sciences
— id: 150635, year: 1986, vol: 483, page: 131, stat: Journal Article,

Interpretation of electron micrographs of adenovirus hexon arrays using a crystallographic molecular model
van Oostrum, J; Smith, P R; Mohraz, M; Burnett, R M
1986 Jul-Sep;96(1-3):77-90, Journal of ultrastructure & molecular structure research
— id: 150637, year: 1986, vol: 96, page: 77, stat: Journal Article,

Novel crystalline sheets of Na,K-ATPase induced by phospholipase A2
Mohraz M; Yee M; Smith PR
1985 Oct-Nov;93(1-2):17-26, Journal of ultrastructure research
— id: 57472, year: 1985, vol: 93, page: 17, stat: Journal Article,

PROGRESS IN THE 3-D STRUCTURE DETERMINATION OF NA-K-ATPASE BY ELECTRON-MICROSCOPY AND IMAGE-PROCESSING
Mohraz, M; Smith, PR
1985 ;47(2):A173-A173, Biophysical journal
— id: 30789, year: 1985, vol: 47, page: A173, stat: Journal Article,

Structure of (Na+,K+)-ATPase as revealed by electron microscopy and image processing
Mohraz M; Smith PR
1984 May;98(5):1836-1841, Journal of cell biology
— id: 57473, year: 1984, vol: 98, page: 1836, stat: Journal Article,

THE STRUCTURE OF (NA+,K+)-ATPASE AS REVEALED BY ELECTRON-MICROSCOPY
MOHRAZ, M; RINDER, CA; SIMPSON, MV; SMITH, PR
1984 ;435(DEC):561-563, Annals of the New York Academy of Sciences
— id: 41242, year: 1984, vol: 435, page: 561, stat: Journal Article,

THE STRUCTURE OF (NA+,K+)-ATPASE AS REVEALED BY ELECTRON-MICROSCOPY
MOHRAZ, M; RINDER, CA; SIMPSON, MV; SMITH, PR
1983 ;97(5):A116-A116, Journal of cell biology
— id: 40606, year: 1983, vol: 97, page: A116, stat: Journal Article,