Barry N Kreiswirth

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Barry Kreiswirth

Adjunct Assistant Professor, Department of Medicine

Contact Info


Research Summary

The Public Health Research Institute TB Center is a multi-faceted laboratory that focuses on the molecular epidemiology of Mycobacterium tuberculosis. Sensitive and multi-drug-resistant clinical isolates are collected from hospitals throughout New York City and genotyped using the transposable element IS6110 as a gene probe. DNA fingerprints are stored and compared using a scanning densitometer with a matching software program that can detect and retrieve identical or like patterns. Multi-drug-resistant strains, first grouped on the basis of IS6110, have been further characterized by sequence analysis of regions of known resistance genes. An example is the characterization of more than 30 mutations in the rpoB gene, which correlate with rifampin resistance. The genetic characterization of the clinical strains provides an ideal test panel to analyze potential anti-tubercular compounds. Compounds received from pharmaceutical houses, biotechnology companies, and basic researchers are analyzed for anti-tubercular activity in both agar- and broth-based systems and in a human macrophage assay. In addition to the applied research, studies on M. tuberculosis pathogenesis are currently underway with the first aim of identifying virulence factors.

Research Interests

Molecular Characterization of Mycobacterium tuberculosis

Early agr activation correlates with vancomycin treatment failure in multi-clonotype MRSA endovascular infections
Abdelhady, Wessam; Chen, Liang; Bayer, Arnold S; Seidl, Kati; Yeaman, Michael R; Kreiswirth, Barry N; Xiong, Yan Q
2015-01-11; 0305-7453,Journal of antimicrobial chemotherapy - id: 1429012, year: 2015 JOURNAL ARTICLE

Frequency and Distribution of Single Nucleotide Polymorphisms within mprF in Methicillin-Resistant Staphylococcus aureus (MRSA) Clinical Isolates: Role in Cross-Resistance between Daptomycin and Host Defense Antimicrobial Peptides
Bayer, Arnold S; Mishra, Nagendra N; Chen, Liang; Kreiswirth, Barry N; Rubio, Aileen; Yang, Soo-Jin
2015-06-15; 1098-6596,Antimicrobial agents & chemotherapy - id: 1626222, year: 2015 JOURNAL ARTICLE

Identification of Outer Membrane and Exoproteins of Carbapenem-Resistant Multilocus Sequence Type 258 Klebsiella pneumoniae
Brinkworth, Amanda J; Hammer, Carl H; Olano, L Renee; Kobayashi, Scott D; Chen, Liang; Kreiswirth, Barry N; DeLeo, Frank R
2015-04-26; 1932-6203,PLoS one - id: 1543092, year: 2015 Journal Article

Complete sequence of a blaKPC-harboring co-integrate plasmid isolated from Escherichia coli
Chavda, Kalyan D; Chen, Liang; Jacobs, Michael R; Rojtman, Albert D; Bonomo, Robert A; Kreiswirth, Barry N
2015-03-16; 0066-4804,Antimicrobial agents & chemotherapy - id: 1494712, year: 2015 JOURNAL ARTICLE

First report of an OXA-48-producing multidrug-resistant Proteus mirabilis strain from Gaza, Palestine
Chen, Liang; Al Laham, Nahed; Chavda, Kalyan D; Mediavilla, Jose R; Jacobs, Michael R; Bonomo, Robert A; Kreiswirth, Barry N
2015-04-26; 1098-6596,Antimicrobial agents & chemotherapy - id: 1543262, year: 2015 JOURNAL ARTICLE