Barry N Kreiswirth

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Barry Kreiswirth

Adjunct Assistant Professor, Department of Medicine

Contact Info

212/263-6479
Barry.Kreiswirth@nyumc.org

Research Summary

The Public Health Research Institute TB Center is a multi-faceted laboratory that focuses on the molecular epidemiology of Mycobacterium tuberculosis. Sensitive and multi-drug-resistant clinical isolates are collected from hospitals throughout New York City and genotyped using the transposable element IS6110 as a gene probe. DNA fingerprints are stored and compared using a scanning densitometer with a matching software program that can detect and retrieve identical or like patterns. Multi-drug-resistant strains, first grouped on the basis of IS6110, have been further characterized by sequence analysis of regions of known resistance genes. An example is the characterization of more than 30 mutations in the rpoB gene, which correlate with rifampin resistance. The genetic characterization of the clinical strains provides an ideal test panel to analyze potential anti-tubercular compounds. Compounds received from pharmaceutical houses, biotechnology companies, and basic researchers are analyzed for anti-tubercular activity in both agar- and broth-based systems and in a human macrophage assay. In addition to the applied research, studies on M. tuberculosis pathogenesis are currently underway with the first aim of identifying virulence factors.

Research Interests

Molecular Characterization of Mycobacterium tuberculosis

Evaluation of the in vitro activity of ceftazidime-avibactam and ceftolozane-tazobactam against meropenem-resistant Pseudomonas aeruginosa isolates
Buehrle, Deanna J; Shields, Ryan K; Chen, Liang; Hao, Binghua; Press, Ellen G; Alkrouk, Ammar; Potoski, Brian A; Kreiswirth, Barry N; Clancy, Cornelius J; Nguyen, M Hong. Evaluation of the in vitro activity of ceftazidime-avibactam and ceftolozane-tazobactam against meropenem-resistant Pseudomonas aeruginosa isolates. Antimicrobial agents & chemotherapy. 2016 Apr 22;60(5):3227-3231 (2047132)

Genomic characterization of Enterobacter cloacae isolates from China that co-produce KPC-3 and NDM-1 carbapenemases
Du, Hong; Chen, Liang; Chavda, Kalyan D; Pandey, Ruchi; Zhang, Haifang; Xie, Xiaofang; Tang, Yi-Wei; Kreiswirth, Barry N. Genomic characterization of Enterobacter cloacae isolates from China that co-produce KPC-3 and NDM-1 carbapenemases. Antimicrobial agents & chemotherapy. 2016 Mar 25;60(4):2519-2523 (2043502)

Emergence of the mcr-1 colistin resistance gene in carbapenem-resistant Enterobacteriaceae
Du, Hong; Chen, Liang; Tang, Yi-Wei; Kreiswirth, Barry N. Emergence of the mcr-1 colistin resistance gene in carbapenem-resistant Enterobacteriaceae [Letter]. Lancet infectious diseases. 2016 Feb 1;16(3):287-288 (2044452)

Genetic Determinants of Drug Resistance in Mycobacterium tuberculosis and Their Diagnostic Value
Farhat, Maha R; Sultana, Razvan; Iartchouk, Oleg; Bozeman, Sam; Galagan, James; Sisk, Peter; Stolte, Christian; Nebenzahl-Guimaraes, Hanna; Jacobson, Karen; Sloutsky, Alexander; Kaur, Devinder; Posey, James; Kreiswirth, Barry N; Kurepina, Natalia; Rigouts, Leen; Streicher, Elizabeth M; Victor, Tommie C; Warren, Robin M; van Soolingen, Dick; Murray, Megan. Genetic Determinants of Drug Resistance in Mycobacterium tuberculosis and Their Diagnostic Value. American journal of respiratory & critical care medicine. 2016 Feb 24;:?-? (2045872)

Phagocytosis and Killing of Carbapenem-Resistant ST258 Klebsiella pneumoniae by Human Neutrophils
Kobayashi, Scott D; Porter, Adeline R; Dorward, David W; Brinkworth, Amanda J; Chen, Liang; Kreiswirth, Barry N; DeLeo, Frank R. Phagocytosis and Killing of Carbapenem-Resistant ST258 Klebsiella pneumoniae by Human Neutrophils. Journal of infectious diseases. 2016 Jan 14;213(10):1615-1622 (2043092)