Barry N Kreiswirth

Biosketch / Results /

Barry Kreiswirth

Adjunct Assistant Professor, Department of Medicine

Contact Info

212/263-6479
Barry.Kreiswirth@nyumc.org

Research Summary

The Public Health Research Institute TB Center is a multi-faceted laboratory that focuses on the molecular epidemiology of Mycobacterium tuberculosis. Sensitive and multi-drug-resistant clinical isolates are collected from hospitals throughout New York City and genotyped using the transposable element IS6110 as a gene probe. DNA fingerprints are stored and compared using a scanning densitometer with a matching software program that can detect and retrieve identical or like patterns. Multi-drug-resistant strains, first grouped on the basis of IS6110, have been further characterized by sequence analysis of regions of known resistance genes. An example is the characterization of more than 30 mutations in the rpoB gene, which correlate with rifampin resistance. The genetic characterization of the clinical strains provides an ideal test panel to analyze potential anti-tubercular compounds. Compounds received from pharmaceutical houses, biotechnology companies, and basic researchers are analyzed for anti-tubercular activity in both agar- and broth-based systems and in a human macrophage assay. In addition to the applied research, studies on M. tuberculosis pathogenesis are currently underway with the first aim of identifying virulence factors.

Research Keywords

molecular characterization of mycobacterium tuberculosis

Comprehensive Genome Analysis of Carbapenemase-Producing Enterobacter spp.: New Insights into Phylogeny, Population Structure, and Resistance Mechanisms
Chavda, Kalyan D; Chen, Liang; Fouts, Derrick E; Sutton, Granger; Brinkac, Lauren; Jenkins, Stephen G; Bonomo, Robert A; Adams, Mark D; Kreiswirth, Barry N. Comprehensive Genome Analysis of Carbapenemase-Producing Enterobacter spp.: New Insights into Phylogeny, Population Structure, and Resistance Mechanisms. MBio. 2016 Dec 13;7(6):?-? (2372092)

Genetic Determinants of Drug Resistance in Mycobacterium tuberculosis and Their Diagnostic Value
Farhat, Maha R; Sultana, Razvan; Iartchouk, Oleg; Bozeman, Sam; Galagan, James; Sisk, Peter; Stolte, Christian; Nebenzahl-Guimaraes, Hanna; Jacobson, Karen; Sloutsky, Alexander; Kaur, Devinder; Posey, James; Kreiswirth, Barry N; Kurepina, Natalia; Rigouts, Leen; Streicher, Elizabeth M; Victor, Tommie C; Warren, Robin M; van Soolingen, Dick; Murray, Megan. Genetic Determinants of Drug Resistance in Mycobacterium tuberculosis and Their Diagnostic Value. American journal of respiratory & critical care medicine. 2016 Sep 01;194(5):621-630 (2045872)

Minimum Inhibitory Concentration of Delamanid (OPC-67683) against Mycobacterium tuberculosis Clinical Isolates and a Proposed Critical Concentration
Stinson, Kelly; Kurepina, Natalia; Venter, Amour; Fujiwara, Mamoru; Kawasaki, Masanori; Timm, Juliano; Shashkina, Elena; Kreiswirth, Barry N; Liu, Yongge; Matsumoto, Makoto; Geiter, Lawrence. Minimum Inhibitory Concentration of Delamanid (OPC-67683) against Mycobacterium tuberculosis Clinical Isolates and a Proposed Critical Concentration. Antimicrobial agents & chemotherapy. 2016 Jun;60(6):3316-3322 (2047142)

Phagocytosis and Killing of Carbapenem-Resistant ST258 Klebsiella pneumoniae by Human Neutrophils
Kobayashi, Scott D; Porter, Adeline R; Dorward, David W; Brinkworth, Amanda J; Chen, Liang; Kreiswirth, Barry N; DeLeo, Frank R. Phagocytosis and Killing of Carbapenem-Resistant ST258 Klebsiella pneumoniae by Human Neutrophils. Journal of infectious diseases. 2016 May 15;213(10):1615-1622 (2043092)

Asymptomatic rectal colonization with carbapenem-resistant Enterobacteriaceae and Clostridium difficile among residents of a long-term care facility in New York City
Prasad, Nishant; Labaze, Georges; Kopacz, Joanna; Chwa, Sophie; Platis, Dimitris; Pan, Cynthia X; Russo, Daniel; LaBombardi, Vincent J; Osorio, Giuliana; Pollack, Simcha; Kreiswirth, Barry N; Chen, Liang; Urban, Carl; Segal-Maurer, Sorana. Asymptomatic rectal colonization with carbapenem-resistant Enterobacteriaceae and Clostridium difficile among residents of a long-term care facility in New York City. American journal of infection control. 2016 May 01;44(5):525-532 (2043672)