Catherine G Klein

Biosketch / Results /

Catherine Klein

Assistant Professor, Department of Environmental Medicine
Dir Mole Cell and Anal Svcs Fac Core NYU NIEHS Ctr
Dir Environmental Health Science Graduate Pgm
Mgr Anal Svs Unit, Exper Anml Exp Core NYUCC

Contact Info

Address
57 Old Forge Rd.
Tuxedo, NY 10987

845/731-3510
Catherine.Klein@nyumc.org

Research Summary

With molecular biologic and molecular cytogenetic techniques, we study the mutagenic and epigenetic mechanisms that silence genes, including transgene expression in a unique set of mammalian cell lines. The G12 and G10 cell lines each contain a bacterial xanthine-guanine phosphoribosyl transferase (gpt) gene target that has been stably inserted into different chromosomal genome locations. These cell lines exhibit differing propensities for mutagenesis, deletions and epigenetic gene silencing by various oxidative carcinogens and clastogens, including x-rays, anticancer therapeutics, metals and traditionally nonmutagenic carcinogens such as diethylstilbestrol (DES). The assay offers the ability to analyze the epigenetic potential of other putative xenoestrogens or environmental toxicants, such as organic pesticides, environmental mixtures and nanoparticles. Using this assay system, the epigenetic silencing of the gpt transgene by DNA condensation, excess methylation and histone modifications were identified as important mechanisms for trangene silencing in carcinogenic nickel-induced G12 mutants. Epigenome arrays add complementary information about other genes and pathways that can be altered by a variety of environmental exposures.

Research Interests

Mechanisms of gene silencing, epigenetics, gene methylation, environmental toxicants, metals

Frontal Cortex Transcriptome Analysis of Mice Exposed to Electronic Cigarettes During Early Life Stages
Lauterstein, Dana E; Tijerina, Pamella B; Corbett, Kevin; Akgol Oksuz, Betul; Shen, Steven S; Gordon, Terry; Klein, Catherine B; Zelikoff, Judith T. Frontal Cortex Transcriptome Analysis of Mice Exposed to Electronic Cigarettes During Early Life Stages. International journal of environmental research & public health. 2016 Apr 12;13(4):417-417 (2078432)

Dysregulation of DNA methylation induced by past arsenic treatment causes persistent genomic instability in mammalian cells
Mauro, Maurizio; Caradonna, Fabio; Klein, Catherine B. Dysregulation of DNA methylation induced by past arsenic treatment causes persistent genomic instability in mammalian cells. Environmental & molecular mutagenesis. 2015 Nov 19;57(2):137-150 (1848602)

Current and future prospectives for jobs in science
Klein, C B. Current and future prospectives for jobs in science [Meeting Abstract]. Environmental & molecular mutagenesis. 2014 Sep;55:S19-S19 (1291802)

Epigenetic assays: Updates, benefits, and limitations
Klein, C B. Epigenetic assays: Updates, benefits, and limitations [Meeting Abstract]. Environmental & molecular mutagenesis. 2014 Sep;55:S27-S27 (1291792)

Nickel
Klein, Catherine; Costa, Max. Nickel IN: Handbook on the toxicology of metals. Amsterdam : Academic Press, 2014 4th ed.. p.1091-1111 (2061012)