Warren R Jelinek

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Warren R Jelinek, Ph.D.

Professor; Chair - IBC Committee
Department of Biochemistry and Molecular Pharmacology

Contact Info

Address
550 First Avenue
Floor 3 Room 346
Medical Science Building
New York, NY 10016

212-263-5937
212-263-8166
Warren.Jelinek@nyumc.org

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Education

1971-1973 — Columbia University, Molecular & Cellular Biology, PostDoctoral Training
— University of Pennsylvania, Graduate Education

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Research Summary

We seek to identify molecules that act during the pre-commitment period to cause the commitment event of terminal differentiation in murine erythroleukemia cells (MELC). We mutagenized MELC by retroviral insertion and isolated mutants that fail to commit to the terminal erythrodifferentiation program. Repeatedly, and independently, one particular genomic locus was the insertional target in fail-to-commit mutants. We now seek to identify the gene associated with this locus. We identified a novel mammalian protein kinase, which we termed STPK13, whose mRNA declines dramatically during the MELC pre-commitment period. Further investigation demonstrated that the mRNA also fluctuates in abundance during the normal cell cycle. Homologous genes, termed polo and cdc5 have recently been isolated from Drosophila and Saccharomyces cerevisiae, respectively. Analyses of mutants of these genes have revealed a requirement during mitosis for the encoded protein kinase. The cell cycle-associated mRNA fluctuations we observed in mouse cells is conserved in S. cerevisiae and thus has been maintained during at least five hundred million years of evolution; it is expected to be a fundamental mechanism of regulation of the protein kinase. Our recent observations suggest that cell cycle-associated regulation of pre-mRNA splicing plays a central role in the cell cycle-associated fluctuation of the abundance of this mRNA. The decline in STPK13 mRNA abundance during the MELC pre-commitment period is expected to indicate an anticipation by these cells of a halt in cell division associated with the commitment event. We recently identified a protein target of association of the STPK13 protein kinase. It is a highly conserved RNA helicase which also shows cell cycle regulation. Both proteins are potentially good candidates as antimitotic targets.

Research Interests

Terminal Differentiation in Murine Erythroleukemia Cells

Research Keywords

cell cycle, differentiation-specific mRNA regulation, mitosis, murine erythroleukemia cells, protein kinase.

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All data from NYU Health Sciences Library Faculty Bibliography — -

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Cell cycle- and terminal differentiation-associated regulation of the mouse mRNA encoding a conserved mitotic protein kinase
Lake RJ; Jelinek WR
1993 Dec;13(12):7793-7801, Molecular & cellular biology
— id: 6432, year: 1993, vol: 13, page: 7793, stat: Journal Article,

Replication of a plasmid bearing a human Alu-family repeat in monkey COS-7 cells
Johnson EM; Jelinek WR
1986 Jul;83(13):4660-4664, Proceedings of the National Academy of Sciences of the United States of America
— id: 57495, year: 1986, vol: 83, page: 4660, stat: Journal Article,

4.5S RNA is encoded by hundreds of tandemly linked genes, has a short half-life, and is hydrogen bonded in vivo to poly(A)-terminated RNAs in the cytoplasm of cultured mouse cells
Schoeniger LO; Jelinek WR
1986 May;6(5):1508-1519, Molecular & cellular biology
— id: 57536, year: 1986, vol: 6, page: 1508, stat: Journal Article,

Repetitive sequence transcripts and U1 RNA in mouse oocytes and eggs
Kaplan G; Jelinek WR; Bachvarova R
1985 May;109(1):15-24, Developmental biology (Orlando)
— id: 17401, year: 1985, vol: 109, page: 15, stat: Journal Article,

ANALYSIS OF RNA SPECIES IN MOUSE OOCYTES BY NORTHERN BLOTTING - ACTIN MESSENGER-RNA, U1-RNA, AND ALU SEQUENCES
BACHVAROVA, R; KAPLAN, G; JELINEK, WR
1983 ;97(5):A20-A20, Journal of cell biology
— id: 40604, year: 1983, vol: 97, page: A20, stat: Journal Article,

Kpn I family of long-dispersed repeated DNA sequences of man: evidence for entry into genomic DNA of DNA copies of poly(A)-terminated Kpn I RNAs
DiGiovanni L; Haynes SR; Misra R; Jelinek WR
1983 Nov;80(21):6533-6537, Proceedings of the National Academy of Sciences of the United States of America
— id: 17402, year: 1983, vol: 80, page: 6533, stat: Journal Article,

The mammalian Alu family of dispersed repeats
Jelinek WR; Haynes SR
1983 ;47 Pt 2:1123-1130, Cold Spring Harbor symposia on quantitative biology
— id: 57494, year: 1983, vol: 47 Pt 2, page: 1123, stat: Journal Article,

Discrete and heterogeneous high molecular weight RNAs complementary to a long dispersed repeat family (a possible transposon) of human DNA
Kole LB; Haynes SR; Jelinek WR
1983 Apr 5;165(2):257-286, Journal of molecular biology
— id: 17403, year: 1983, vol: 165, page: 257, stat: Journal Article,

Repetitive sequences in eukaryotic DNA and their expression
Jelinek WR; Schmid CW
1982 ;51(4550):813-844, Annual review of biochemistry
— id: 17405, year: 1982, vol: 51, page: 813, stat: Journal Article,

The Alu family of dispersed repetitive sequences
Schmid CW; Jelinek WR
1982 Jun 4;216(4550):1065-1070, Science
— id: 17404, year: 1982, vol: 216, page: 1065, stat: Journal Article,

Low molecular weight RNAs transcribed in vitro by RNA polymerase III from Alu-type dispersed repeats in Chinese hamster DNA are also found in vivo
Haynes SR; Jelinek WR
1981 Oct;78(10):6130-6134, Proceedings of the National Academy of Sciences of the United States of America
— id: 17406, year: 1981, vol: 78, page: 6130, stat: Journal Article,

The Chinese hamster Alu-equivalent sequence: a conserved highly repetitious, interspersed deoxyribonucleic acid sequence in mammals has a structure suggestive of a transposable element
Haynes SR; Toomey TP; Leinwand L; Jelinek WR
1981 Jul;1(7):573-583, Molecular & cellular biology
— id: 17407, year: 1981, vol: 1, page: 573, stat: Journal Article,

Ubiquitous, interspersed repeated sequences in mammalian genomes
Jelinek WR; Toomey TP; Leinwand L; Duncan CH; Biro PA; Choudary PV; Weissman SM; Rubin CM; Houck CM; Deininger PL; Schmid CW
1980 Mar;77(3):1398-1402, Proceedings of the National Academy of Sciences of the United States of America
— id: 17408, year: 1980, vol: 77, page: 1398, stat: Journal Article,

Isolation and sequence determination of 5'-terminal oligonucleotide fragments of RNA transcripts synthesized by bacteriophage T3-induced RNA polymerase from T3 DNA
Maitra, U; Jelinek, W; Yudelevich, A; Majumder, H K; Guha, A
1980 Jul;77(7):3908-3911, Proceedings of the National Academy of Sciences of the United States of America
— id: 178939, year: 1980, vol: 77, page: 3908, stat: Journal Article,

In vitro RNA-RNA splicing in adenovirus 2 mRNA formation
Blanchard, J M; Weber, J; Jelinek, W; Darnell, J E
1978 Nov;75(11):5344-5348, Proceedings of the National Academy of Sciences of the United States of America
— id: 178940, year: 1978, vol: 75, page: 5344, stat: Journal Article,

Inverted repeated DNA from Chinese hamster ovary cells studied with cloned DNA fragments
Jelinek WR
1978 Jun;75(6):2679-2683, Proceedings of the National Academy of Sciences of the United States of America
— id: 17409, year: 1978, vol: 75, page: 2679, stat: Journal Article,

Oligonucleotides in heterogeneous nuclear RNA: similarity of inverted repeats and RNA from repetitious DNA sites
Jelinek, W; Evans, R; Wilson, M; Salditt-Georgieff, M; Darnell, J E
1978 Jul;17(14):2776-2783, Biochemistry
— id: 178942, year: 1978, vol: 17, page: 2776, stat: Journal Article,

Low molecular weight RNAs hydrogen-bonded to nuclear and cytoplasmic poly(A)-terminated RNA from cultured Chinese hamster ovary cells
Jelinek, W; Leinwand, L
1978 Sep;15(1):205-214, Cell
— id: 178941, year: 1978, vol: 15, page: 205, stat: Journal Article,

Mapping of inverted repeated DNA sequences within the genome of simian virus 40
Hsu MT; Jelinek WR
1977 Apr;74(4):1631-1634, Proceedings of the National Academy of Sciences of the United States of America
— id: 17411, year: 1977, vol: 74, page: 1631, stat: Journal Article,

Specific nucleotide sequences in HeLa cell inverted repeated DNA: enrichment for sequences found in double-stranded regions of heterogeneous nuclear RNA
Jelinek WR
1977 Oct 5;115(4):591-601, Journal of molecular biology
— id: 17410, year: 1977, vol: 115, page: 591, stat: Journal Article,

Characterization of vesicular stomatitis virus mRNA species synthesized in vitro
Rhodes, D P; Abraham, G; Colonno, R J; Jelinek, W; Banerjee, A K
1977 Mar;21(3):1105-1112, Journal of virology
— id: 178916, year: 1977, vol: 21, page: 1105, stat: Journal Article,

Determination of nucleotide sequences from double-stranded regions of HeLa cell nuclear RNA
Robertson, H D; Dickson, E; Jelinek, W
1977 Oct;115(4):571-589, Journal of molecular biology
— id: 178943, year: 1977, vol: 115, page: 571, stat: Journal Article,

3'-Terminal addition to HeLa cell nuclear and cytoplasmic poly (A)
Sawicki, S G; Jelinek, W; Darnell, J E
1977 Jun;113(1):219-235, Journal of molecular biology
— id: 178944, year: 1977, vol: 113, page: 219, stat: Journal Article,

The definition of a large viral transcription unit late in Ad2 infection of HeLa cells: mapping of nascent RNA molecules labeled in isolated nuclei
Weber, J; Jelinek, W; Darnell, J E Jr
1977 Apr;10(4):611-616, Cell
— id: 178945, year: 1977, vol: 10, page: 611, stat: Journal Article,

Biochemical events in mRNA formation in mammalian cells
Darnell JE; Jelinek W; Puckett L; Derman E; Bachenheimer S
1976 ;194(34):53-74, Symposium of the Society for Developmental Biology
— id: 23912, year: 1976, vol: 194, page: 53, stat: Journal Article,

Methyl labeling of HeLa cell hnRNA: a comparison with mRNA
Salditt-Georgieff, M; Jelinek, W; Darnell, J E; Furuichi, Y; Morgan, M; Shatkin, A
1976 Feb;7(2):227-237, Cell
— id: 178946, year: 1976, vol: 7, page: 227, stat: Journal Article,

Methylated, blocked 5 termini in HeLa cell mRNA
Furuichi, Y; Morgan, M; Shatkin, A J; Jelinek, W; Salditt-Georgieff, M; Darnell, J E
1975 May;72(5):1904-1908, Proceedings of the National Academy of Sciences of the United States of America
— id: 178947, year: 1975,