Michael John Garabedian

Biosketch / Results /

Michael Garabedian

Professor, Department of Microbiology
Professor, Department of Urology

Course Director

Contact Info

Address
450 East 29th Street
New York, NY 10016

212/263-7662
Michael.Garabedian@med.nyu.edu

Research Summary

Steroid hormone receptors are ligand-regulated transcription factors that control diverse physiological and developmental processes. In addition, estrogen and androgen receptors (ER and AR) are implicated in the etiology of breast and prostate cancer, respectively, whereas the glucocorticoid receptor (GR) is an important therapeutic target for cancer and inflammatory diseases. The goal of my laboratory is to elucidate the mechanism of signal transduction and transcriptional regulation by these receptors. Specifically, the lab is interested: 1) How molecular chaperones regulate steroid receptor action; 2) how transcriptional regulatory molecules (coactivators and corepressors) modulate ER, GR and AR transcriptional activity and the role of steroid receptor phosphorylation in the recruitment of coactivators and corepressors, 3) how Rho GTPases and the actin cytoskeleton regulate steroid receptor transcriptional response, and 4) the mechanism of GR-induced cell growth arrest and apoptosis. We are using genetic (mouse knock-outs and knock-ins), biochemical (microarrays and high-throughput screening of small molecules that disrupt receptor:coactivator interaction) as well as cell biological strategies (studying macromolecular complexes using color variants of GFP) to elucidate the mechanism of ER, AR and GR function in vivo. Understanding the mechanism of steroid receptor-regulated gene expression may reveal novel points of intervention to be exploited in the development of new therapies for steroid-dependent malignancies, such as breast and prostate cancer.

 

Research Keywords

signal transduction and transcriptional regulation by steroid receptors, molecular, cellular, & translational neuroscience

URI Regulates KAP1 Phosphorylation and Transcriptional Repression Via PP2A Phosphatase in Prostate Cancer Cells
Mita, Paolo; Savas, Jeffrey N; Briggs, Erica M; Ha, Susan; Gnanakkan, Veena; Yates, John R 3rd; Robins, Diane M; David, Gregory; Boeke, Jef D; Garabedian, Michael J; Logan, Susan K. URI Regulates KAP1 Phosphorylation and Transcriptional Repression Via PP2A Phosphatase in Prostate Cancer Cells. Journal of biological chemistry. 2016 Dec 02;291(49):25516-25528 (2288712)

PPARgamma agonists promote differentiation of cancer stem cells by restraining YAP transcriptional activity
Basu-Roy, Upal; Han, Eugenia; Rattanakorn, Kirk; Gadi, Abhilash; Verma, Narendra; Maurizi, Giulia; Gunaratne, Preethi H; Coarfa, Cristian; Kennedy, Oran D; Garabedian, Michael J; Basilico, Claudio; Mansukhani, Alka. PPARgamma agonists promote differentiation of cancer stem cells by restraining YAP transcriptional activity. Oncotarget. 2016 Sep 20;7(38):60954-60970 (2219342)

Multivalent peptoid conjugates suppress enzalutamide-resistant prostate cancer cellular proliferation
Wang, Yu; Dehigaspitiya, Dilani C; Levine, Paul M; Profit, Adam A; Haugbro, Michael; Imberg-Kazdan, Keren; Logan, Susan K; Kirshenbaum, Kent; Garabedian, Michael J. Multivalent peptoid conjugates suppress enzalutamide-resistant prostate cancer cellular proliferation. Cancer research. 2016 Sep 01;76(17):5124-5132 (2199512)

Dynein axonemal heavy chain 8 promotes androgen receptor activity and associates with prostate cancer progression
Wang, Yu; Ledet, Russell J; Imberg-Kazdan, Keren; Logan, Susan K; Garabedian, Michael J. Dynein axonemal heavy chain 8 promotes androgen receptor activity and associates with prostate cancer progression. Oncotarget. 2016 Aug 2;7(31):49268-49280 (2167082)

PARP-1 Represses LXR-mediated ABCA1 Expression and Cholesterol Efflux in Macrophages
Shrestha, Elina; Hussein, Maryem A; Savas, Jeffery N; Ouimet, Mireille; Barrett, Tessa J; Leone, Sarah; Yates, John R 3rd; Moore, Kathryn J; Fisher, Edward A; Garabedian, Michael J. PARP-1 Represses LXR-mediated ABCA1 Expression and Cholesterol Efflux in Macrophages. Journal of biological chemistry. 2016 May 20;291(21):11172-11184 (2059152)