Michael John Garabedian

Biosketch / Results /

Michael Garabedian

Professor, Department of Microbiology;Professor, Department of Urology

Contact Info

522 First Avenue
New York, NY 10016



1987-1993 — Dr. Garabedian was a Postdoctoral Fellow in the Department of Biochemistry and Biophysics at the University of California, San Francisco, CA, PostDoctoral Training
— Brandeis University, Graduate Education

Research Summary

Steroid hormone receptors are ligand-regulated transcription factors that control diverse physiological and developmental processes. In addition, estrogen and androgen receptors (ER and AR) are implicated in the etiology of breast and prostate cancer, respectively, whereas the glucocorticoid receptor (GR) is an important therapeutic target for cancer and inflammatory diseases. The goal of my laboratory is to elucidate the mechanism of signal transduction and transcriptional regulation by these receptors. Specifically, the lab is interested: 1) How molecular chaperones regulate steroid receptor action; 2) how transcriptional regulatory molecules (coactivators and corepressors) modulate ER, GR and AR transcriptional activity and the role of steroid receptor phosphorylation in the recruitment of coactivators and corepressors, 3) how Rho GTPases and the actin cytoskeleton regulate steroid receptor transcriptional response, and 4) the mechanism of GR-induced cell growth arrest and apoptosis. We are using genetic (mouse knock-outs and knock-ins), biochemical (microarrays and high-throughput screening of small molecules that disrupt receptor:coactivator interaction) as well as cell biological strategies (studying macromolecular complexes using color variants of GFP) to elucidate the mechanism of ER, AR and GR function in vivo. Understanding the mechanism of steroid receptor-regulated gene expression may reveal novel points of intervention to be exploited in the development of new therapies for steroid-dependent malignancies, such as breast and prostate cancer.

Research Interests

Signal Transduction and Transcriptional Regulation by Steroid Receptors

Modulation of macrophage gene expression via LXRalpha serine 198 phosphorylation
Wu, Chaowei; Hussein, Maryem; Shrestha, Elina; Leone, Sarah; Aiyegbo, Mohammed S; Lambert, W Marcus; Pourcet, Benoit; Cardozo, Timothy; Gustaffson, Jan-Ake; Fisher, Edward A; Pineda-Torra, Ines; Garabedian, Michael J
2015-04-06; 1098-5549,Molecular & cellular biology - id: 1519242, year: 2015 JOURNAL ARTICLE

Loss of TBL1XR1 Disrupts Glucocorticoid Receptor Recruitment to Chromatin and Results in Glucocorticoid Resistance in a B-Lymphoblastic Leukemia Model
Jones, Courtney L; Bhatla, Teena; Blum, Roy; Wang, Jinhua; Paugh, Steven W; Wen, Xin; Bourgeois, Wallace; Bitterman, Danielle S; Raetz, Elizabeth A; Morrison, Debra J; Teachey, David T; Evans, William E; Garabedian, Michael J; Carroll, William L
2014-06-06; 0021-9258,Journal of biological chemistry - id: 1030982, year: 2014 JOURNAL ARTICLE

MAPK Signaling Cascades Mediate Distinct Glucocorticoid Resistance Mechanisms in Pediatric B-Precursor ALL
Jones, Courtney L; Gearheart, Christy M; Fosmire, Susan; Delgado-Martin, Cristina; Pais, Faye; Wang, Jinhua; Bhatla, Teena; Bitterman, Danielle S; de Rijk, Simone R; Bourgeois, Wallace; Dandekar, Smita C; Raetz, Elizabeth A; Hermiston, Michelle L; Garabedian, Michael J; Porter, Christopher C; Carroll, William L
2015-03-19; 1528-0020,Blood - id: 1497612, year: 2014

Targeting the Androgen Receptor with Steroid Conjugates
Levine, Paul M; Garabedian, Michael J; Kirshenbaum, Kent
2014-06-20; 0022-2623,Journal of medicinal chemistry - id: 1036692, year: 2014 JOURNAL ARTICLE

Caveolin-1 Regulates Genomic Action of the Glucocorticoid Receptor in Neural Stem Cells
Peffer, Melanie E; Chandran, Uma R; Luthra, Soumya; Volonte, Daniela; Galbiati, Ferruccio; Garabedian, Michael J; Monaghan, A Paula; Defranco, Donald B
2014-05-02; 0270-7306,Molecular & cellular biology - id: 941102, year: 2014 JOURNAL ARTICLE