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Daniel J Eichinger

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Daniel J Eichinger, Ph.D.

Associate Professor; Graduate Advisor & Chair of DAPC
Department of Microbiology (Microbiology )

Contact Info

Address
341 E. 25th St, OPH-307
Floor 1,3 Room 107b (Of), 307 (Lab)
Old Public Health
New York, NY 10010

Phone: 212-263-8171
Fax: 212-263-8116
Email: Daniel.Eichinger@nyumc.org

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Education

1986 — Dr. Eichinger received his Ph.D., degree in Parasitology from New York University Medical Center, New York, NY, Graduate Education
1986-1989 — Dr. Eichinger was a Postdoctoral Fellow in Molecular Biology and Genetics at Johns Hopkins University School of Medicine in Baltimore, MD, PostDoctoral Training

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Research Summary

Entamoeba histolytica is a protozoan infecting up to 10% of the human population and causing approximately 50 million cases of invasive colitis each year. Infection begins with the ingestion of the parasite1s cyst form, which transforms into the disease-causing ameboid trophozoite in the colon. Reconversion of trophozoites into the infective cyst completes the life cycle. Primarily, we study cyst formation at the protein and gene expression levels to identify cyst-inducing conditions and the parasite products which sense these conditions. Using a reptilian Entamoeba parasite, which readily encysts in vitro, we apply several approaches to isolate genes and their products which are expressed during cyst formation. We also explore culture conditions which induce excystation, and we are developing a DNA transfection system; both will permit a detailed study of gene expression during the complete parasite life cycle. We will use information from this model system to target future studies of therapies that will hopefully interrupt progression of the E. histolytica life cycle.

Another interest is a peculiar type of sialidase expressed by the parasite Trypanosoma cruzi. This enzyme can remove sialic acid from the terminal position of a glycoconjugate and reattach the sugar to another galactose-terminated carbohydrate molecule. The enzyme thus functions as a sialidase, similar to viral and bacterial enyzmes, and as a glycosyltransferase that uses preformed carbohydrate molecules as substrates. We are conducting structure and function studies of this trans-sialidase to map the enzymatic domain required for the unique sialic acid transferase function.

Research Interests

Pathogenic Mechanisms of Protozoan Parasites

Research Keywords

Entameoba, encystation, sialidase, trans-sialidase, trypanosome

Grant Keywords

Foodborne Pathogens, Gastrointestinal Diseases/Disorders, Tropical Diseases, Infectious Diseases/Agents, Digestive System

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All data from NYU Health Sciences Library Faculty Bibliography — -

Contact:
http://hsl.med.nyu.edu/faculty-bibliography-search#about

Compounds of the upper gastrointestinal tract induce rapid and efficient excystation of Entamoeba invadens
Mitra, Biswa Nath; Pradel, Gabriele; Frevert, Ute; Eichinger, Daniel
2010 May;40(6):751-760, International journal for parasitology
— id: 109037, year: 2010, vol: 40, page: 751, stat: Journal Article,

CHARACTERIZATION OF THE IMMUNE RESPONSE TO THE LARVAL PHASE OF ASCARIS SUUM INFECTION AND IDENTIFICATION OF ASCARIS ANTIGENS EXPRESSED DURING THAT PHASE
Fernandez, MAG; Pollack, HJ; Eichinger, D; Hotez, PJ
2009 NOV ;81(5):134-134, American journal of tropical medicine & hygiene
— id: 106986, year: 2009, vol: 81, page: 134, stat: Journal Article,

Analysis of commercial Entamoeba histolytica ELISA kits for the detection of Entamoeba invadens in reptiles
Brewer, Laurie A; Denver, Mary C; Whitney, Meredith; Eichinger, Daniel J
2008 Sep;39(3):493-495, Journal of zoo & wildlife medicine
— id: 95552, year: 2008, vol: 39, page: 493, stat: Journal Article,

Acetylation of the Entamoeba histone H4 N-terminal domain is influenced by short-chain fatty acids that enter trophozoites in a pH-dependent manner
Byers, Jennifer; Eichinger, Daniel
2008 Jan;38(1):57-64, International journal for parasitology
— id: 78787, year: 2008, vol: 38, page: 57, stat: Journal Article,

Trichostatin A effects on gene expression in the protozoan parasite Entamoeba histolytica
Ehrenkaufer, Gretchen M; Eichinger, Daniel J; Singh, Upinder
2007 ;8:216-216, BMC genomics
— id: 78788, year: 2007, vol: 8, page: 216, stat: Journal Article,

Identification of developmentally regulated genes in Entamoeba histolytica: insights into mechanisms of stage conversion in a protozoan parasite
Ehrenkaufer, Gretchen M; Haque, Rashidul; Hackney, Jason A; Eichinger, Daniel J; Singh, Upinder
2007 Jun;9(6):1426-1444, Cellular microbiology
— id: 78789, year: 2007, vol: 9, page: 1426, stat: Journal Article,

A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries
Teixeira, Marcia C A; Oliveira, Geraldo G S; Silvany, Marco A; Alcantara-Neves, Neuza M; Soares, Milena B P; Ribeiro-Dos-Santos, Ricardo; Jeronimo, Selma M B; Costa, Carlos H; dos-Santos, Washington L C; Eichinger, Daniel; Pontes-de-Carvalho, Lain
2007 Mar;35(1):51-54, Biologicals
— id: 78790, year: 2007, vol: 35, page: 51, stat: Journal Article,

Entamoeba invadens: the requirement for galactose ligands during encystment
Turner, N A; Eichinger, D
2007 Aug;116(4):467-474, Experimental parasitology
— id: 73292, year: 2007, vol: 116, page: 467, stat: Journal Article,

Entamoeba invadens: restriction of ploidy by colonic short chain fatty acids
Byers, Jennifer; Eichinger, Daniel
2005 Jul;110(3):203-206, Experimental parasitology
— id: 78791, year: 2005, vol: 110, page: 203, stat: Journal Article,

Colonic short-chain fatty acids inhibit encystation of Entamoeba invadens
Byers, Jennifer; Faigle, Wolfgang; Eichinger, Daniel
2005 Feb;7(2):269-279, Cellular microbiology
— id: 56028, year: 2005, vol: 7, page: 269, stat: Journal Article,

Parasite-derived trans-sialidase binds to heart tissue in Trypanosoma cruzi-infected animals
Alcantara-Neves, Neuza M; Ribeiro-dos-Santos, Ricardo; Amor, Ana Lucia Moreno; Uemura, Haruki; Silva-Neto, Samuel J; Eichinger, Daniel; Pontes-de-Carvalho, Lain
2004 Nov;37(5):273-278, Microbial pathogenesis
— id: 78792, year: 2004, vol: 37, page: 273, stat: Journal Article,

Entamoeba invadens contains the components of a classical adrenergic signaling system
Frederick, Jesse; Eichinger, Dan
2004 Oct;137(2):339-343, Molecular & biochemical parasitology
— id: 51385, year: 2004, vol: 137, page: 339, stat: Journal Article,

Gene discovery in the Entamoeba invadens genome
Wang, Zheng; Samuelson, John; Clark, C Graham; Eichinger, Daniel; Paul, Jaishree; Van Dellen, Katrina; Hall, Neil; Anderson, Iain; Loftus, Brendan
2003 Jun;129(1):23-31, Molecular & biochemical parasitology
— id: 78793, year: 2003, vol: 129, page: 23, stat: Journal Article,

The enteric parasite Entamoeba uses an autocrine catecholamine system during differentiation into the infectious cyst stage
Coppi, Alida; Merali, Salim; Eichinger, Daniel
2002 Mar 8;277(10):8083-8090, Journal of biological chemistry
— id: 39730, year: 2002, vol: 277, page: 8083, stat: Journal Article,

Catecholamines in Entamoebae: recent (re)discoveries
Eichinger, D; Coppi, A; Frederick, J; Merali, S
2002 NOV ;27(6):589-593, Journal of biosciences
— id: 33282, year: 2002, vol: 27, page: 589, stat: Journal Article,

A role for a galactose lectin and its ligands during encystment of Entamoeba
Eichinger D
2001 Jan-Feb;48(1):17-21, Journal of eukaryotic microbiology
— id: 26778, year: 2001, vol: 48, page: 17, stat: Journal Article,

Encystation in parasitic protozoa
Eichinger D
2001 Aug;4(4):421-426, Current opinion in microbiology
— id: 26705, year: 2001, vol: 4, page: 421, stat: Journal Article,

Characterization of the cell adhesion site of Trypanosoma cruzi metacyclic stage surface glycoprotein gp82
Manque PM; Eichinger D; Juliano MA; Juliano L; Araya JE; Yoshida N
2000 Feb;68(2):478-484, Infection & immunity
— id: 57565, year: 2000, vol: 68, page: 478, stat: Journal Article,

Regulation of Entamoeba invadens encystation and gene expression with galactose and N-acetylglucosamine
Coppi A; Eichinger D
1999 Jul 30;102(1):67-77, Molecular & biochemical parasitology
— id: 6253, year: 1999, vol: 102, page: 67, stat: Journal Article,

Proteasome-dependent cyst formation and stage-specific ubiquitin mRNA accumulation in Entamoeba invadens
Gonzalez J; Bai G; Frevert U; Corey EJ; Eichinger D
1999 Sep;264(3):897-904, European journal of biochemistry
— id: 8485, year: 1999, vol: 264, page: 897, stat: Journal Article,

Crithidia fasciculata induces encystation of Entamoeba invadens in a galactose-dependent manner
Cho J; Eichinger D
1998 Aug;84(4):705-710, Journal of parasitology
— id: 12080, year: 1998, vol: 84, page: 705, stat: Journal Article,

Encystation of entamoeba parasites
Eichinger D
1997 Jul;19(7):633-639, Bioessays
— id: 8216, year: 1997, vol: 19, page: 633, stat: Journal Article,

Proteasome function is required for encystation of Entamoeba invadens
Gonzalez J; Frevert U; Corey EJ; Nussenzweig V; Eichinger D
1997 ;28 Spec No:139-140, Archives of medical research (Mexico)
— id: 12421, year: 1997, vol: 28 Spec No, page: 139, stat: Journal Article,

Temperature differences for trans-glycosylation and hydrolysis reaction reveal an acceptor binding site in the catalytic mechanism of Trypanosoma cruzi trans-sialidase
Ribeirao, M; Pereira-Chioccola, V L; Eichinger, D; Rodrigues, M M; Schenkman, S
1997 Dec;7(8):1237-1246, Glycobiology
— id: 78794, year: 1997, vol: 7, page: 1237, stat: Journal Article,

Single immunizing dose of recombinant adenovirus efficiently induces CD8+ T cell-mediated protective immunity against malaria
Rodrigues EG; Zavala F; Eichinger D; Wilson JM; Tsuji M
1997 Feb 1;158(3):1268-1274, Journal of immunology
— id: 8435, year: 1997, vol: 158, page: 1268, stat: Journal Article,

Directed mutagenesis of the Trypanosoma cruzi trans-sialidase enzyme identifies two domains involved in its sialyltransferase activity
Smith LE; Eichinger D
1997 Apr;7(3):445-451, Glycobiology
— id: 56509, year: 1997, vol: 7, page: 445, stat: Journal Article,

Isolation and expression of an open reading frame encoding sialidase from Trypanosoma rangeli
Smith LE; Uemura H; Eichinger D
1996 Jul;79(1):21-33, Molecular & biochemical parasitology
— id: 56892, year: 1996, vol: 79, page: 21, stat: Journal Article,

Trans-sialidase genes expressed in mammalian forms of Trypanosoma cruzi evolved from ancestor genes expressed in insect forms of the parasite
Briones, M R; Egima, C M; Eichinger, D; Schenkman, S
1995 Aug;41(2):120-131, Journal of molecular evolution
— id: 78795, year: 1995, vol: 41, page: 120, stat: Journal Article,

Identification of a developmentally regulated transcript expressed during encystation of Entamoeba invadens
Sanchez L; Enea V; Eichinger D
1994 Sep;67(1):125-135, Molecular & biochemical parasitology
— id: 56703, year: 1994, vol: 67, page: 125, stat: Journal Article,

Increased levels of polyadenylated histone H2B mRNA accumulate during Entamoeba invadens cyst formation
Sanchez LB; Enea V; Eichinger D
1994 Sep;67(1):137-146, Molecular & biochemical parasitology
— id: 56704, year: 1994, vol: 67, page: 137, stat: Journal Article,

Structural and functional properties of Trypanosoma trans-sialidase
Schenkman S; Eichinger D; Pereira ME; Nussenzweig V
1994 ;48:499-523, Annual review of microbiology
— id: 56524, year: 1994, vol: 48, page: 499, stat: Journal Article,

A proteolytic fragment of Trypanosoma cruzi trans-sialidase lacking the carboxyl-terminal domain is active, monomeric, and generates antibodies that inhibit enzymatic activity
Schenkman, S; Chaves, L B; Pontes de Carvalho, L C; Eichinger, D
1994 Mar 18;269(11):7970-7975, Journal of biological chemistry
— id: 78796, year: 1994, vol: 269, page: 7970, stat: Journal Article,

Demonstration of heat-shock protein 70 in the sporozoite stage of malaria parasites
Tsuji M; Mattei D; Nussenzweig RS; Eichinger D; Zavala F
1994 ;80(1):16-21, Parasitology research
— id: 13020, year: 1994, vol: 80, page: 16, stat: Journal Article,

Trypanosoma cruzi trans-sialidase and cell invasion
Schenkman, S; Eichinger, D
1993 Jun;9(6):218-222, Parasitology today
— id: 78797, year: 1993, vol: 9, page: 218, stat: Journal Article,

Only some members of a gene family in Trypanosoma cruzi encode proteins that express both trans-sialidase and neuraminidase activities
Uemura H; Schenkman S; Nussenzweig V; Eichinger D
1992 Nov;11(11):3837-3844, EMBO journal
— id: 61957, year: 1992, vol: 11, page: 3837, stat: Journal Article,

Circumsporozoite protein of Plasmodium berghei: gene cloning and identification of the immunodominant epitopes
Eichinger DJ; Arnot DE; Tam JP; Nussenzweig V; Enea V
1986 Nov;6(11):3965-3972, Molecular & cellular biology
— id: 61980, year: 1986, vol: 6, page: 3965, stat: Journal Article,

The murine sex-limited protein (Slp): reassessment of its sex limitation
Ferreira A; Eichinger D; Nussenzweig V
1982 Oct;129(4):1506-1508, Journal of immunology
— id: 62014, year: 1982, vol: 129, page: 1506, stat: Journal Article,

A SIMPLIFIED TWO-DIMENSIONAL ELECTROPHORETIC TECHNIQUE
FERREIRA, A; EICHINGER, D
1981 ;43(3):291-299, Journal of immunological methods
— id: 40324, year: 1981, vol: 43, page: 291, stat: Journal Article,