Biosketch / Results /

Ramanuj Dasgupta

Research Associate Professor, Department of Biochemistry and Molecular Pharmacology
Perlmutter Cancer Center

Contact Info

Address
522 First Avenue
New York, NY 10016

212/263-9247
Ramanuj.Dasgupta@nyumc.org

Education

— University of Chicago, Graduate Education

Research Interests

Finally, an important aspect of our research will be the combination of RNAi technology with small molecule screens in the hope of isolating more specific drug targets for the Wnt pathway. Though identifying specific targets of small molecules is always a challenge, our approach of comparing and integrating information about specific phenotypes from the RNAi and the small molecule screens, should aid in making target identification faster. This approach will allow us to not only propose new testable hypotheses about the mechanisms of the regulators of the Wnt-signaling pathway, but also in drug discovery with relevance to treatment and prevention of Wnt pathway-related diseases.|Finally, an important aspect of our research will be the combination of RNAi technology with small molecule screens in the hope of isolating more specific drug targets for the Wnt pathway. Though identifying specific targets of small molecules is always a challenge, our approach of comparing and integrating information about specific phenotypes from the RNAi and the small molecule screens, should aid in making target identification faster. This approach will allow us to not only propose new testable hypotheses about the mechanisms of the regulators of the Wnt-signaling pathway, but also in drug discovery with relevance to treatment and prevention of Wnt pathway-related diseases.|The Wnt/Wingless (Wg) pathway is one of a core set of evolutionarily conserved signaling pathways that regulates many aspects of animal development. Aberrant Wnt signaling has been linked to human disease, such as cancers of the intestine, stomach, breast, liver, and skin. Mutations in the Wnt genes or in those that encode regulators of this pathway can cause devastating birth defects, including debilitating abnormalities of the central nervous system, axial skeleton, limbs, and occasionally other organs.|The Wnt/Wingless (Wg) pathway is one of a core set of evolutionarily conserved signaling pathways that regulates many aspects of animal development. Aberrant Wnt signaling has been linked to human disease, such as cancers of the intestine, stomach, breast, liver, and skin. Mutations in the Wnt genes or in those that encode regulators of this pathway can cause devastating birth defects, including debilitating abnormalities of the central nervous system, axial skeleton, limbs, and occasionally other organs.|The focus of my laboratory is to integrate a variety of functional genomic and proteomic high-throughput screens to generate a global picture of how the Wnt signaling pathway is regulated at a molecular level. We are also interested in understanding how components of the Wnt pathway may interact with other signal transduction cascades during development and disease. We are using a newly developed technology, called RNA-interference (RNAi) to systematically knockdown gene function on a genome scale and testing the effects (phenotype) of reduced gene function on cells and in the developing embryo. The RNAi screens are used to assign new function to genes in the context of the Wnt pathway.|The focus of my laboratory is to integrate a variety of functional genomic and proteomic high-throughput screens to generate a global picture of how the Wnt signaling pathway is regulated at a molecular level. We are also interested in understanding how components of the Wnt pathway may interact with other signal transduction cascades during development and disease. We are using a newly developed technology, called RNA-interference (RNAi) to systematically knockdown gene function on a genome scale and testing the effects (phenotype) of reduced gene function on cells and in the developing embryo. The RNAi screens are used to assign new function to genes in the context of the Wnt pathway.

Wnt inhibition leads to improved chemosensitivity in paediatric acute lymphoblastic leukaemia
Dandekar, Smita; Romanos-Sirakis, Eleny; Pais, Faye; Bhatla, Teena; Jones, Courtney; Bourgeois, Wallace; Hunger, Stephen P; Raetz, Elizabeth A; Hermiston, Michelle L; Dasgupta, Ramanuj; Morrison, Debra J; Carroll, William L
2014-07-09; 0007-1048,British journal of haematology - id: 1066072, year: 2014 JOURNAL ARTICLE

Wnt Co-receptor Lrp5 is a Driver of Idiopathic Pulmonary Fibrosis
Lam, Anna P; Herazo-Maya, Jose D; Sennello, Joseph A; Flozak, Annette S; Russell, Susan; Mutlu, Gokhan M; Budinger, G R Scott; DasGupta, Ramanuj; Varga, John; Kaminski, Naftali; Gottardi, Cara J
2014-06-14; 1073-449x,American journal of respiratory & critical care medicine - id: 1033782, year: 2014 JOURNAL ARTICLE

Luciferase reporter assay in Drosophila and mammalian tissue culture cells
Yun, Chi; Dasgupta, Ramanuj
2014-05-12; 2160-4762,Current protocols in chemical biology - id: 970062, year: 2014 Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

A membrane-associated beta-catenin/Oct4 complex correlates with ground-state pluripotency in mouse embryonic stem cells
Faunes, Fernando; Hayward, Penelope; Descalzo, Silvia Munoz; Chatterjee, Sujash S; Balayo, Tina; Trott, Jamie; Christoforou, Andrew; Ferrer-Vaquer, Anna; Hadjantonakis, Anna-Katerina; Dasgupta, Ramanuj; Arias, Alfonso Martinez
2013-03-27; 0950-1991,Development - id: 249552, year: 2013 Journal Article

Inhibition of androgen receptor and beta-catenin activity in prostate cancer
Lee, Eugine; Madar, Aviv; David, Gregory; Garabedian, Michael J; Dasgupta, Ramanuj; Logan, Susan K
2013-09-30; 0027-8424,Proceedings of the National Academy of Sciences of the United States of America (PNAS) - id: 542762, year: 2013 Journal Article