Nicholas J Cowan

Biosketch / Results /

Nicholas J Cowan, Ph.D.

Professor;
Departments of Biochemistry and Molecular Pharmacology (Biochemistry) and Biochemistry and Molecular Pharmacology

Contact Info

Address
550 First Avenue
Department of Biochemistry Floor 3 Room 315
Medical Science Building
New York, NY 10016

212-263-8166
Nicholas.Cowan@nyumc.org

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Education

1967-1970 — University of Oxford, England, PhD in Biochemistry, Graduate Education
1970-1973 — Medical Research Council Laboratory of Molecular Biology, Cambridge, England, PostDoctoral Training

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Research Summary

The final stage in the flow of information from encoded DNA to expressed proteins is the folding of each protein into its correct three-dimensional structure. In principle, such folding reactions can occur spontaneously because all the necessary information required to determine proper folding is contained within the primary amino acid sequence. However, under physiological conditions, constraints of temperature and the tendency of unfolded proteins to aggregate are such that many proteins must undergo facilitated folding via interaction with one or more protein complexes termed chaperonins. We are concerned with the mechanism whereby proteins in the eukaryotic cytosol, such as actin and tubulin, are folded via interaction with the cytoplasmic chaperonin, a heteromeric double toroid that provides a sequestered environment in which the unscrambling of aberrantly folded target proteins can occur. We are studying the nature of target proteins that interact with the chaperonin, the function of various cofactors that modulate the folding reaction, and the role of ATP hydrolysis in chaperonin-mediated folding.

Research Interests

Protein Folding in the Eukaryotic Cytosol

Research Keywords

actin, chaperonins, cytoskeleton, protein folding, tubulin

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All data from NYU Health Sciences Library Faculty Bibliography — -

Contact:
http://hsl.med.nyu.edu/faculty-bibliography-search

Mutations in TUBG1, DYNC1H1, KIF5C and KIF2A cause malformations of cortical development and microcephaly
Poirier, Karine; Lebrun, Nicolas; Broix, Loic; Tian, Guoling; Saillour, Yoann; Boscheron, Cecile; Parrini, Elena; Valence, Stephanie; Pierre, Benjamin Saint; Oger, Madison; Lacombe, Didier; Genevieve, David; Fontana, Elena; Darra, Franscesca; Cances, Claude; Barth, Magalie; Bonneau, Dominique; Bernadina, Bernardo Dalla; N'guyen, Sylvie; Gitiaux, Cyril; Parent, Philippe; des Portes, Vincent; Pedespan, Jean Michel; Legrez, Victoire; Castelnau-Ptakine, Laetitia; Nitschke, Patrick; Hieu, Thierry; Masson, Cecile; Zelenika, Diana; Andrieux, Annie; Francis, Fiona; Guerrini, Renzo; Cowan, Nicholas J; Bahi-Buisson, Nadia; Chelly, Jamel
2013 Apr;45(6):639-647, Nature genetics
— id: 394952, year: 2013, vol: 45, page: 639, stat: Journal Article,

Tubulin-Specific Chaperones: Components of a Molecular Machine That Assembles the alpha/beta Heterodimer
Tian, Guoling; Cowan, Nicholas J
2013 ;115:155-171, Methods in cell biology
— id: 512962, year: 2013, vol: 115, page: 155, stat: Journal Article,

Mutations in the beta-tubulin gene TUBB5 cause microcephaly with structural brain abnormalities
Breuss, Martin; Heng, Julian Ik-Tsen; Poirier, Karine; Tian, Guoling; Jaglin, Xavier Hubert; Qu, Zhengdong; Braun, Andreas; Gstrein, Thomas; Ngo, Linh; Haas, Matilda; Bahi-Buisson, Nadia; Moutard, Marie-Laure; Passemard, Sandrine; Verloes, Alain; Gressens, Pierre; Xie, Yunli; Robson, Kathryn J H; Rani, Deepa Selvi; Thangaraj, Kumarasamy; Clausen, Tim; Chelly, Jamel; Cowan, Nicholas Justin; Keays, David Anthony
2012 Dec;2(6):1554-1562, Cell reports
— id: 249192, year: 2012, vol: 2, page: 1554, stat: Journal Article,

Regulation of Androgen Receptor-Mediated Transcription by RPB5 Binding Protein URI/RMP
Mita, Paolo; Savas, Jeffrey N; Djouder, Nabil; Yates, John R 3rd; Ha, Susan; Ruoff, Rachel; Schafler, Eric D; Nwachukwu, Jerome C; Tanese, Naoko; Cowan, Nicholas J; Zavadil, Jiri; Garabedian, Michael J; Logan, Susan K
2011 Sep;31(17):3639-3652, Molecular & cellular biology
— id: 136514, year: 2011, vol: 31, page: 3639, stat: Journal Article,

Tuba8 Is Expressed at Low Levels in the Developing Mouse and Human Brain
Braun, A; Breuss, M; Salzer, MC; Flint, J; Cowan, NJ; Keays, DA
2010 MAY 14 ;86(5):819-822, American journal of human genetics
— id: 110005, year: 2010, vol: 86, page: 819, stat: Journal Article,

Disease-associated mutations in TUBA1A result in a spectrum of defects in the tubulin folding and heterodimer assembly pathway
Tian, Guoling; Jaglin, Xavier H; Keays, David A; Francis, Fiona; Chelly, Jamel; Cowan, Nicholas J
2010 Sep 15;19(18):3599-3613, Human molecular genetics
— id: 112037, year: 2010, vol: 19, page: 3599, stat: Journal Article,

Effect of TBCD and its regulatory interactor Arl2 on tubulin and microtubule integrity
Tian, Guoling; Thomas, Simi; Cowan, Nicholas J
2010 Nov;67(11):706-714, Cytoskeleton (Hoboken)
— id: 113945, year: 2010, vol: 67, page: 706, stat: Journal Article,

Mutations in the beta-tubulin gene TUBB2B result in asymmetrical polymicrogyria
Jaglin, Xavier Hubert; Poirier, Karine; Saillour, Yoann; Buhler, Emmanuelle; Tian, Guoling; Bahi-Buisson, Nadia; Fallet-Bianco, Catherine; Phan-Dinh-Tuy, Francoise; Kong, Xiang Peng; Bomont, Pascale; Castelnau-Ptakhine, Laetitia; Odent, Sylvie; Loget, Philippe; Kossorotoff, Manoelle; Snoeck, Irina; Plessis, Ghislaine; Parent, Philippe; Beldjord, Cherif; Cardoso, Carlos; Represa, Alfonso; Flint, Jonathan; Keays, David Anthony; Cowan, Nicholas Justin; Chelly, Jamel
2009 Jun;41(6):746-752, Nature genetics
— id: 135247, year: 2009, vol: 41, page: 746, stat: Journal Article,

A Pachygyria-causing {alpha}-Tubulin Mutation Results in Inefficient Cycling with CCT and a Deficient Interaction with TBCB
Tian, Guoling; Kong, Xiang-Peng; Jaglin, Xavier H; Chelly, Jamel; Keays, David; Cowan, Nicholas J
2008 Mar;19(3):1152-1161, Molecular biology of the cell
— id: 78375, year: 2008, vol: 19, page: 1152, stat: Journal Article,

Mutations in alpha-tubulin cause abnormal neuronal migration in mice and lissencephaly in humans
Keays, David A; Tian, Guoling; Poirier, Karine; Huang, Guo-Jen; Siebold, Christian; Cleak, James; Oliver, Peter L; Fray, Martin; Harvey, Robert J; Molnar, Zoltan; Pinon, Maria C; Dear, Neil; Valdar, William; Brown, Steve D M; Davies, Kay E; Rawlins, J Nicholas P; Cowan, Nicholas J; Nolan, Patrick; Chelly, Jamel; Flint, Jonathan
2007 Jan 12;128(1):45-57, Cell
— id: 78376, year: 2007, vol: 128, page: 45, stat: Journal Article,

Cryptic out-of-frame translational initiation of TBCE rescues tubulin formation in compound heterozygous HRD
Tian, Guoling; Huang, Melissa C; Parvari, Ruti; Diaz, George A; Cowan, Nicholas J
2006 Sep 5;103(36):13491-13496, Proceedings of the National Academy of Sciences of the United States of America
— id: 67543, year: 2006, vol: 103, page: 13491, stat: Journal Article,

Mutations affecting beta-tubulin folding and degradation
Wang, Yaqing; Tian, Guoling; Cowan, Nicholas J; Cabral, Fernando
2006 May 12;281(19):13628-13635, Journal of biological chemistry
— id: 67544, year: 2006, vol: 281, page: 13628, stat: Journal Article,

Identification of a novel tubulin-destabilizing protein related to the chaperone cofactor E
Bartolini, Francesca; Tian, Guoling; Piehl, Michelle; Cassimeris, Lynne; Lewis, Sally A; Cowan, Nicholas J
2005 Mar 15;118(Pt 6):1197-1207, Journal of cell science
— id: 56011, year: 2005, vol: 118, page: 1197, stat: Journal Article,

Selective contribution of eukaryotic prefoldin subunits to actin and tubulin binding
Simons, C Torrey; Staes, An; Rommelaere, Heidi; Ampe, Christophe; Lewis, Sally A; Cowan, Nicholas J
2004 Feb 6;279(6):4196-4203, Journal of biological chemistry
— id: 42643, year: 2004, vol: 279, page: 4196, stat: Journal Article,

Cytosolic Arl2 is complexed with cofactor D and protein phosphatase 2A
Shern, Jack F; Sharer, J Daniel; Pallas, David C; Bartolini, Francesca; Cowan, Nicholas J; Reed, Matthew S; Pohl, Jan; Kahn, Richard A
2003 Oct 17;278(42):40829-40836, Journal of biological chemistry
— id: 48114, year: 2003, vol: 278, page: 40829, stat: Journal Article,

Functional overlap between retinitis pigmentosa 2 protein and the tubulin-specific chaperone cofactor C
Bartolini, Francesca; Bhamidipati, Arunashree; Thomas, Scott; Schwahn, Uwe; Lewis, Sally A; Cowan, Nicholas J
2002 Apr 26;277(17):14629-14634, Journal of biological chemistry
— id: 67547, year: 2002, vol: 277, page: 14629, stat: Journal Article,

Localization in the human retina of the X-linked retinitis pigmentosa protein RP2, its homologue cofactor C and the RP2 interacting protein Arl3
Grayson, Celene; Bartolini, Francesca; Chapple, J Paul; Willison, Keith R; Bhamidipati, Arunashree; Lewis, Sally A; Luthert, Philip J; Hardcastle, Alison J; Cowan, Nicholas J; Cheetham, Michael E
2002 Nov 15;11(24):3065-3074, Human molecular genetics
— id: 67546, year: 2002, vol: 11, page: 3065, stat: Journal Article,

Bad chaperone
Lewis, Sally A; Cowan, Nicholas J
2002 Nov;8(11):1202-1203, Nature medicine
— id: 63336, year: 2002, vol: 8, page: 1202, stat: Journal Article,

Structure of eukaryotic prefoldin and of its complexes with unfolded actin and the cytosolic chaperonin CCT
Martin-Benito, Jaime; Boskovic, Jasminka; Gomez-Puertas, Paulino; Carrascosa, Jose L; Simons, C Torrey; Lewis, Sally A; Bartolini, Francesca; Cowan, Nicholas J; Valpuesta, Jose M
2002 Dec 2;21(23):6377-6386, EMBO journal
— id: 67545, year: 2002, vol: 21, page: 6377, stat: Journal Article,

Type II chaperonins, prefoldin, and the tubulin-specific chaperones
Cowan NJ; Lewis SA
2001 ;59(2):73-104, Advances in protein chemistry
— id: 32463, year: 2001, vol: 59, page: 73, stat: Journal Article,

ADP ribosylation factor-like protein 2 (Arl2) regulates the interaction of tubulin-folding cofactor D with native tubulin
Bhamidipati A; Lewis SA; Cowan NJ
2000 May 29;149(5):1087-1096, Journal of cell biology
— id: 11674, year: 2000, vol: 149, page: 1087, stat: Journal Article,

The development of auditory attention in children
Gomes, H; Molholm, S; Christodoulou, C; Ritter, W; Cowan, N
2000 Jan 1;5:D108-D120, Frontiers in biosciences
— id: 129227, year: 2000, vol: 5, page: D108, stat: Journal Article,

Mismatch negativity in children and adults, and effects of an attended task
Gomes, H; Molholm, S; Ritter, W; Kurtzberg, D; Cowan, N; Vaughan, H G Jr
2000 Nov;37(6):807-816, Psychophysiology
— id: 129228, year: 2000, vol: 37, page: 807, stat: Journal Article,

Purification of prefoldin
Lewis, S A; Cowan, N J
2000 ;140:179-184, Methods in molecular biology
— id: 78378, year: 2000, vol: 140, page: 179, stat: Journal Article,

A chaperone with a hydrophilic surface
Cowan NJ; Lewis SA
1999 Nov;6(11):990-991, Nature structural biology
— id: 11910, year: 1999, vol: 6, page: 990, stat: Journal Article,

Prefoldin-nascent chain complexes in the folding of cytoskeletal proteins
Hansen WJ; Cowan NJ; Welch WJ
1999 Apr 19;145(2):265-277, Journal of cell biology
— id: 17132, year: 1999, vol: 145, page: 265, stat: Journal Article,

A single-ring mitochondrial chaperonin (Hsp60-Hsp10) can substitute for GroEL-GroES in vivo
Nielsen KL; McLennan N; Masters M; Cowan NJ
1999 Sep;181(18):5871-5875, Journal of bacteriology
— id: 6201, year: 1999, vol: 181, page: 5871, stat: Journal Article,

Tubulin folding cofactors as GTPase-activating proteins. GTP hydrolysis and the assembly of the alpha/beta-tubulin heterodimer
Tian G; Bhamidipati A; Cowan NJ; Lewis SA
1999 Aug 20;274(34):24054-24058, Journal of biological chemistry
— id: 6176, year: 1999, vol: 274, page: 24054, stat: Journal Article,

Mammalian cytosolic chaperonin
Cowan NJ
1998 ;290:230-241, Methods in enzymology
— id: 12142, year: 1998, vol: 290, page: 230, stat: Journal Article,

Cytoskeletal protein-prefoldin chaperone complexes form co-translationally en route to the cytosolic chaperonin and folding to the native state
Hansen, WJ; Cowan, NJ; Welch, WJ
1998 NOV ;9(11):453A-453A, Molecular biology of the cell
— id: 53651, year: 1998, vol: 9, page: 453A, stat: Journal Article,

A single ring is sufficient for productive chaperonin-mediated folding in vivo
Nielsen KL; Cowan NJ
1998 Jul;2(1):93-99, Molecular cell
— id: 7711, year: 1998, vol: 2, page: 93, stat: Journal Article,

Prefoldin, a chaperone that delivers unfolded proteins to cytosolic chaperonin
Vainberg IE; Lewis SA; Rommelaere H; Ampe C; Vandekerckhove J; Klein HL; Cowan NJ
1998 May 29;93(5):863-873, Cell
— id: 7835, year: 1998, vol: 93, page: 863, stat: Journal Article,

The alpha- and beta-tubulin folding pathways
Lewis, S A; Tian, G; Cowan, N J
1997 Dec;7(12):479-484, Trends in cell biology
— id: 78377, year: 1997, vol: 7, page: 479, stat: Journal Article,

The ?- and ?-tubulin folding pathways
Lewis, SA; Tian, G; Cowan, NJ
1997 ;7(12):479-484, Trends in cell biology
— id: 589682, year: 1997, vol: 7, page: 479, stat: Journal Article,

Tubulin subunits exist in an activated conformational state generated and maintained by protein cofactors
Tian G; Lewis SA; Feierbach B; Stearns T; Rommelaere H; Ampe C; Cowan NJ
1997 Aug 25;138(4):821-832, Journal of cell biology
— id: 7270, year: 1997, vol: 138, page: 821, stat: Journal Article,

Chaperonin-mediated folding of actin and tubulin
Lewis SA; Tian G; Vainberg IE; Cowan NJ
1996 Jan;132(1-2):1-4, Journal of cell biology
— id: 6929, year: 1996, vol: 132, page: 1, stat: Journal Article,

GFAP is necessary for the integrity of CNS white matter architecture and long-term maintenance of myelination
Liedtke W; Edelmann W; Bieri PL; Chiu FC; Cowan NJ; Kucherlapati R; Raine CS
1996 Oct;17(4):607-615, Neuron
— id: 17133, year: 1996, vol: 17, page: 607, stat: Journal Article,

Pathway leading to correctly folded beta-tubulin
Tian G; Huang Y; Rommelaere H; Vandekerckhove J; Ampe C; Cowan NJ
1996 Jul 26;86(2):287-296, Cell
— id: 56896, year: 1996, vol: 86, page: 287, stat: Journal Article,

Quasi-native chaperonin-bound intermediates in facilitated protein folding
Tian G; Vainberg IE; Tap WD; Lewis SA; Cowan NJ
1995 Oct 13;270(41):23910-23913, Journal of biological chemistry
— id: 6872, year: 1995, vol: 270, page: 23910, stat: Journal Article,

Specificity in chaperonin-mediated protein folding
Tian G; Vainberg IE; Tap WD; Lewis SA; Cowan NJ
1995 May 18;375(6528):250-253, Nature
— id: 56720, year: 1995, vol: 375, page: 250, stat: Journal Article,

A novel cochaperonin that modulates the ATPase activity of cytoplasmic chaperonin
Gao Y; Melki R; Walden PD; Lewis SA; Ampe C; Rommelaere H; Vandekerckhove J; Cowan NJ
1994 Jun;125(5):989-996, Journal of cell biology
— id: 6381, year: 1994, vol: 125, page: 989, stat: Journal Article,

Facilitated folding of actins and tubulins occurs via a nucleotide-dependent interaction between cytoplasmic chaperonin and distinctive folding intermediates
Melki R; Cowan NJ
1994 May;14(5):2895-2904, Molecular & cellular biology
— id: 17134, year: 1994, vol: 14, page: 2895, stat: Journal Article,

THE DEVELOPMENT OF CATEGORIES AT THE SEMANTICS SYNTAX INTERFACE
BRAINE, MDS; BROOKS, PJ; COWAN, N; SAMUELS, MC; TAMISLEMONDA, C
1993 OCT-DEC ;8(4):465-494, Cognitive development
— id: 98446, year: 1993, vol: 8, page: 465, stat: Journal Article,

Two cofactors and cytoplasmic chaperonin are required for the folding of alpha- and beta-tubulin
Gao Y; Vainberg IE; Chow RL; Cowan NJ
1993 Apr;13(4):2478-2485, Molecular & cellular biology
— id: 13211, year: 1993, vol: 13, page: 2478, stat: Journal Article,

Chaperonin-mediated folding of vertebrate actin-related protein and gamma-tubulin
Melki R; Vainberg IE; Chow RL; Cowan NJ
1993 Sep;122(6):1301-1310, Journal of cell biology
— id: 6454, year: 1993, vol: 122, page: 1301, stat: Journal Article,

Eukaryotic cytosolic chaperonin contains t-complex polypeptide 1 and seven related subunits
Rommelaere H; Van Troys M; Gao Y; Melki R; Cowan NJ; Vandekerckhove J; Ampe C
1993 Dec 15;90(24):11975-11979, Proceedings of the National Academy of Sciences of the United States of America
— id: 17135, year: 1993, vol: 90, page: 11975, stat: Journal Article,

A novel 205-kilodalton testis-specific serine/threonine protein kinase associated with microtubules of the spermatid manchette
Walden PD; Cowan NJ
1993 Dec;13(12):7625-7635, Molecular & cellular biology
— id: 6548, year: 1993, vol: 13, page: 7625, stat: Journal Article,

The mechanism of equilibrium binding of microtubule-associated protein 2 to microtubules. Binding is a multi-phasic process and exhibits positive cooperativity
Wallis KT; Azhar S; Rho MB; Lewis SA; Cowan NJ; Murphy DB
1993 Jul 15;268(20):15158-15167, Journal of biological chemistry
— id: 17136, year: 1993, vol: 268, page: 15158, stat: Journal Article,

Projection domains of MAP2 and tau determine spacings between microtubules in dendrites and axons
Chen J; Kanai Y; Cowan NJ; Hirokawa N
1992 Dec 17;360(6405):674-677, Nature
— id: 17137, year: 1992, vol: 360, page: 674, stat: Journal Article,

DIFFERENTIAL FUNCTIONS OF MAP2, MAP2C AND TAU ON THE ORGANIZATION OF MICROTUBULES IN THE CELLS
CHEN, J; KANAI, Y; COWAN, N; HIROKAWA, N
1992 SEP ;3(4):A259-A259, Molecular biology of the cell
— id: 51865, year: 1992, vol: 3, page: A259, stat: Journal Article,

A cytoplasmic chaperonin that catalyzes beta-actin folding
Gao Y; Thomas JO; Chow RL; Lee GH; Cowan NJ
1992 Jun 12;69(6):1043-1050, Cell
— id: 13562, year: 1992, vol: 69, page: 1043, stat: Journal Article,

Increased microtubule stability and alpha tubulin acetylation in cells transfected with microtubule-associated proteins MAP1B, MAP2 or tau
Takemura R; Okabe S; Umeyama T; Kanai Y; Cowan NJ; Hirokawa N
1992 Dec;103(Pt 4):953-964, Journal of cell science
— id: 17138, year: 1992, vol: 103, page: 953, stat: Journal Article,

Mammalian mitochondrial chaperonin 60 functions as a single toroidal ring
Viitanen PV; Lorimer GH; Seetharam R; Gupta RS; Oppenheim J; Thomas JO; Cowan NJ
1992 Jan 15;267(2):695-698, Journal of biological chemistry
— id: 8274, year: 1992, vol: 267, page: 695, stat: Journal Article,

Tubulin dimer formation via the release of alpha- and beta-tubulin monomers from multimolecular complexes
Zabala JC; Cowan NJ
1992 ;23(3):222-230, Cell motility & the cytoskeleton
— id: 17139, year: 1992, vol: 23, page: 222, stat: Journal Article,

THE NEURONAL CYTOSKELETON - BURGOYNE,RO
COWAN, N
1991 AUG 15 ;352(6336):580-580, Nature
— id: 51568, year: 1991, vol: 352, page: 580, stat: Journal Article,

Regulation of expression of glial filament acidic protein
Sarkar S; Cowan NJ
1991 ;15:97-102, Journal of cell science. Supplement
— id: 14205, year: 1991, vol: 15, page: 97, stat: Journal Article,

INTRAGENIC SEQUENCES AFFECT THE EXPRESSION OF THE GENE ENCODING GLIAL FIBRILLARY ACIDIC PROTEIN
Sarkar, S; Cowan, NJ
1991 Aug;57(2):675-684, Journal of neurochemistry
— id: 32165, year: 1991, vol: 57, page: 675, stat: Journal Article,

Microtubule bundling
Lewis, S A; Cowan, N
1990 Jun;345(6277):674-674, Nature
— id: 582172, year: 1990, vol: 345, page: 674, stat: Journal Article,

Assembly properties of altered beta-tubulin polypeptides containing disrupted autoregulatory domains
Gu W; Cowan NJ
1989 Aug;9(8):3418-3428, Molecular & cellular biology
— id: 10532, year: 1989, vol: 9, page: 3418, stat: Journal Article,

Organization of microtubules in dendrites and axons is determined by a short hydrophobic zipper in microtubule-associated proteins MAP2 and tau
Lewis SA; Ivanov IE; Lee GH; Cowan NJ
1989 Nov 30;342(6249):498-505, Nature
— id: 10427, year: 1989, vol: 342, page: 498, stat: Journal Article,

The microtubule binding domain of microtubule-associated protein MAP1B contains a repeated sequence motif unrelated to that of MAP2 and tau
Noble M; Lewis SA; Cowan NJ
1989 Dec;109(6 Pt 2):3367-3376, Journal of cell biology
— id: 10424, year: 1989, vol: 109, page: 3367, stat: Journal Article,

Nerve growth factor regulates both the phosphorylation and steady-state levels of microtubule-associated protein 1.2 (MAP1.2)
Aletta JM; Lewis SA; Cowan NJ; Greene LA
1988 May;106(5):1573-1581, Journal of cell biology
— id: 11102, year: 1988, vol: 106, page: 1573, stat: Journal Article,

Differential distribution of beta-tubulin isotypes in cerebellum
Burgoyne RD; Cambray-Deakin MA; Lewis SA; Sarkar S; Cowan NJ
1988 Aug;7(8):2311-2319, EMBO journal
— id: 17140, year: 1988, vol: 7, page: 2311, stat: Journal Article,

TUBULIN ISOTYPES AND THEIR INTERACTION WITH MICROTUBULE ASSOCIATED PROTEINS
Cowan, NJ; Lewis, SA; Wei, G; Burgoyne, RD
1988 Sep;145(2-3):106-111, Protoplasma
— id: 31591, year: 1988, vol: 145, page: 106, stat: Journal Article,

Generation of antisera that discriminate among mammalian alpha-tubulins: introduction of specialized isotypes into cultured cells results in their coassembly without disruption of normal microtubule function
Gu W; Lewis SA; Cowan NJ
1988 Jun;106(6):2011-2022, Journal of cell biology
— id: 11078, year: 1988, vol: 106, page: 2011, stat: Journal Article,

The primary structure and heterogeneity of tau protein from mouse brain
Lee, G; Cowan, N; Kirschner, M
1988 Jan;239(4837):285-288, Science
— id: 163416, year: 1988, vol: 239, page: 285, stat: Journal Article,

THE PRIMARY STRUCTURE AND HETEROGENEITY OF TAU-PROTEIN FROM MOUSE-BRAIN
LEE, G; COWAN, N; KIRSCHNER, M
1988 JAN 15 ;239(4837):285-288, Science
— id: 41864, year: 1988, vol: 239, page: 285, stat: Journal Article,

Complex regulation and functional versatility of mammalian alpha- and beta-tubulin isotypes during the differentiation of testis and muscle cells
Lewis SA; Cowan NJ
1988 Jun;106(6):2023-2033, Journal of cell biology
— id: 11077, year: 1988, vol: 106, page: 2023, stat: Journal Article,

Microtubule-associated protein MAP2 shares a microtubule binding motif with tau protein
Lewis SA; Wang DH; Cowan NJ
1988 Nov 11;242(4880):936-939, Science
— id: 10886, year: 1988, vol: 242, page: 936, stat: Journal Article,

Complete sequence of a cDNA encoding mouse MAP2
Wang D; Lewis SA; Cowan NJ
1988 Dec 9;16(23):11369-11370, Nucleic acids research
— id: 10862, year: 1988, vol: 16, page: 11369, stat: Journal Article,

FUNCTION AND REGULATION OF EXPRESSION OF THE GENES ENCODING NEUROFILAMENT PROTEIN AND THE GLIAL FIBRILLARY ACIDIC PROTEIN
Cowan, NJ; Levy, E; Sarkan, S; Gordon, J; Lewis, S
1987 May 1;46(6):2145-2145, Federation proceedings (Federation of American Societies for Experimental Biology)
— id: 31361, year: 1987, vol: 46, page: 2145, stat: Journal Article,

Neurofilament gene expression: a major determinant of axonal caliber
Hoffman PN; Cleveland DW; Griffin JW; Landes PW; Cowan NJ; Price DL
1987 May;84(10):3472-3476, Proceedings of the National Academy of Sciences of the United States of America
— id: 17143, year: 1987, vol: 84, page: 3472, stat: Journal Article,

Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular-mass neurofilament protein
Levy E; Liem RK; D'Eustachio P; Cowan NJ
1987 Jul 1;166(1):71-77, European journal of biochemistry
— id: 17141, year: 1987, vol: 166, page: 71, stat: Journal Article,

Free intermingling of mammalian beta-tubulin isotypes among functionally distinct microtubules
Lewis SA; Gu W; Cowan NJ
1987 May 22;49(4):539-548, Cell
— id: 17142, year: 1987, vol: 49, page: 539, stat: Journal Article,

Anomalous placement of introns in a member of the intermediate filament multigene family: an evolutionary conundrum
Lewis SA; Cowan NJ
1986 May;6(5):1529-1534, Molecular & cellular biology
— id: 17148, year: 1986, vol: 6, page: 1529, stat: Journal Article,

Tubulin pseudogenes as markers for hominoid divergence
Lewis SA; Cowan NJ
1986 Feb 20;187(4):623-626, Journal of molecular biology
— id: 17149, year: 1986, vol: 187, page: 623, stat: Journal Article,

A cloned cDNA encoding MAP1 detects a single copy gene in mouse and a brain-abundant RNA whose level decreases during development
Lewis SA; Sherline P; Cowan NJ
1986 Jun;102(6):2106-2114, Journal of cell biology
— id: 17146, year: 1986, vol: 102, page: 2106, stat: Journal Article,

Brain-specific expression of MAP2 detected using a cloned cDNA probe
Lewis SA; Villasante A; Sherline P; Cowan NJ
1986 Jun;102(6):2098-2105, Journal of cell biology
— id: 17147, year: 1986, vol: 102, page: 2098, stat: Journal Article,

Six mouse alpha-tubulin mRNAs encode five distinct isotypes: testis-specific expression of two sister genes
Villasante A; Wang D; Dobner P; Dolph P; Lewis SA; Cowan NJ
1986 Jul;6(7):2409-2419, Molecular & cellular biology
— id: 17145, year: 1986, vol: 6, page: 2409, stat: Journal Article,

The mammalian beta-tubulin repertoire: hematopoietic expression of a novel, heterologous beta-tubulin isotype
Wang D; Villasante A; Lewis SA; Cowan NJ
1986 Nov;103(5):1903-1910, Journal of cell biology
— id: 17144, year: 1986, vol: 103, page: 1903, stat: Journal Article,

Structure of the mouse glial fibrillary acidic protein gene: implications for the evolution of the intermediate filament multigene family
Balcarek JM; Cowan NJ
1985 Aug 12;13(15):5527-5543, Nucleic acids research
— id: 17152, year: 1985, vol: 13, page: 5527, stat: Journal Article,

Structural implications of a cDNA clone encoding mouse glial fibrillary acidic protein
Cowan NJ; Lewis SA; Balcarek JM; Krek V; Shelanski M
1985 ;455(1):575-582, Annals of the New York Academy of Sciences
— id: 17156, year: 1985, vol: 455, page: 575, stat: Journal Article,

THE PHONOLOGICAL AND METAPHONOLOGICAL REPRESENTATION OF SPEECH - EVIDENCE FROM FLUENT BACKWARD TALKERS
COWAN, N; BRAINE, MDS; LEAVITT, LA
1985 JAN 20 ;24(6):679-698, Journal of memory & language
— id: 98566, year: 1985, vol: 24, page: 679, stat: Journal Article,

Structural features and restricted expression of a human alpha-tubulin gene
Hall JL; Cowan NJ
1985 Jan 11;13(1):207-223, Nucleic acids research
— id: 17155, year: 1985, vol: 13, page: 207, stat: Journal Article,

Genetics, evolution, and expression of the 68,000-mol-wt neurofilament protein: isolation of a cloned cDNA probe
Lewis SA; Cowan NJ
1985 Mar;100(3):843-850, Journal of cell biology
— id: 17154, year: 1985, vol: 100, page: 843, stat: Journal Article,

Temporal expression of mouse glial fibrillary acidic protein mRNA studied by a rapid in situ hybridization procedure
Lewis SA; Cowan NJ
1985 Sep;45(3):913-919, Journal of neurochemistry
— id: 17151, year: 1985, vol: 45, page: 913, stat: Journal Article,

Three expressed sequences within the human beta-tubulin multigene family each define a distinct isotype
Lewis SA; Gilmartin ME; Hall JL; Cowan NJ
1985 Mar 5;182(1):11-20, Journal of molecular biology
— id: 17153, year: 1985, vol: 182, page: 11, stat: Journal Article,

Five mouse tubulin isotypes and their regulated expression during development
Lewis SA; Lee MG; Cowan NJ
1985 Sep;101(3):852-861, Journal of cell biology
— id: 17150, year: 1985, vol: 101, page: 852, stat: Journal Article,

Tubulin genes and the diversity of microtubule function
Cowan NJ
1984 ;1(9):36-60, Oxford surveys on eukaryotic genes
— id: 17159, year: 1984, vol: 1, page: 36, stat: Journal Article,

SALIENT CHILDHOOD MEMORIES
COWAN, N; DAVIDSON, G
1984 JAN 20 ;145(1):101-107, Journal of genetic psychology
— id: 98589, year: 1984, vol: 145, page: 101, stat: Journal Article,

Sequence of an expressed human beta-tubulin gene containing ten Alu family members
Lee MG; Loomis C; Cowan NJ
1984 Jul 25;12(14):5823-5836, Nucleic acids research
— id: 17157, year: 1984, vol: 12, page: 5823, stat: Journal Article,

Sequence of a cDNA clone encoding mouse glial fibrillary acidic protein: structural conservation of intermediate filaments
Lewis SA; Balcarek JM; Krek V; Shelanski M; Cowan NJ
1984 May;81(9):2743-2746, Proceedings of the National Academy of Sciences of the United States of America
— id: 17158, year: 1984, vol: 81, page: 2743, stat: Journal Article,

Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions
Cowan NJ; Dobner PR; Fuchs EV; Cleveland DW
1983 Oct;3(10):1738-1745, Molecular & cellular biology
— id: 17160, year: 1983, vol: 3, page: 1738, stat: Journal Article,

Tubulin isotypes and the multigene tubulin families
Cowan NJ; Dudley L
1983 ;85(5):147-173, International review of cytology
— id: 17163, year: 1983, vol: 85, page: 147, stat: Journal Article,

Identification of two human beta-tubulin isotypes
Hall JL; Dudley L; Dobner PR; Lewis SA; Cowan NJ
1983 May;3(5):854-862, Molecular & cellular biology
— id: 17162, year: 1983, vol: 3, page: 854, stat: Journal Article,

Evolutionary history of a multigene family: an expressed human beta-tubulin gene and three processed pseudogenes
Lee MG; Lewis SA; Wilde CD; Cowan NJ
1983 Jun;33(2):477-487, Cell
— id: 17161, year: 1983, vol: 33, page: 477, stat: Journal Article,

Structure of two human alpha-tubulin genes
Wilde CD; Chow LT; Wefald FC; Cowan NJ
1982 Jan;79(1):96-100, Proceedings of the National Academy of Sciences of the United States of America
— id: 17167, year: 1982, vol: 79, page: 96, stat: Journal Article,

Diverse mechanisms in the generation of human beta-tubulin pseudogenes
Wilde CD; Crowther CE; Cowan NJ
1982 Aug 6;217(4559):549-549, Science
— id: 17164, year: 1982, vol: 217, page: 549, stat: Journal Article,

Isolation of a multigene family containing human alpha-tubulin sequences
Wilde CD; Crowther CE; Cowan NJ
1982 Mar 15;155(4):533-538, Journal of molecular biology
— id: 17166, year: 1982, vol: 155, page: 533, stat: Journal Article,

Evidence that a human beta-tubulin pseudogene is derived from its corresponding mRNA
Wilde CD; Crowther CE; Cripe TP; Gwo-Shu Lee M; Cowan NJ
1982 May 6;297(5861):83-84, Nature
— id: 17165, year: 1982, vol: 297, page: 83, stat: Journal Article,

Structural variation among human beta-tubulin genes
Cowan NJ; Wilde CD; Chow LT; Wefald FC
1981 Aug;78(8):4877-4881, Proceedings of the National Academy of Sciences of the United States of America
— id: 17168, year: 1981, vol: 78, page: 4877, stat: Journal Article,

Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes
Cleveland DW; Lopata MA; MacDonald RJ; Cowan NJ; Rutter WJ; Kirschner MW
1980 May;20(1):95-105, Cell
— id: 17169, year: 1980, vol: 20, page: 95, stat: Journal Article,

Isolation of separate mRNAs for alpha- and beta-tubulin and characterization of the corresponding in vitro translation products
Cleveland DW; Kirschner MW; Cowan NJ
1978 Nov;15(3):1021-1031, Cell
— id: 17170, year: 1978, vol: 15, page: 1021, stat: Journal Article,

Expression of antibody genes in tissue culture: structural mutants and hybrid cells
Milstein C; Adetugbo K; Cowan NJ; Kohler G; Secher DS
1978 May;15(48):321-330, National Cancer Institute monograph
— id: 17171, year: 1978, vol: 15, page: 321, stat: Journal Article,

Somatic cell genetics of antibody-secreting cells: studies of clonal diversification and analysis by cell fusion
Milstein C; Adetugbo K; Cowan NJ; Kohler G; Secher DS; Wilde CD
1977 ;41 Pt 2(48):793-803, Cold Spring Harbor symposia on quantitative biology
— id: 17172, year: 1977, vol: 41 Pt 2, page: 793, stat: Journal Article,

Purification and sequence analysis of the mRNA coding for an immunoglobulin heavy chain
Cowan NJ; Secher DS; Milstein C
1976 Jan 15;61(2):353-368, European journal of biochemistry
— id: 17173, year: 1976, vol: 61, page: 353, stat: Journal Article,

Stability of cytoplasmic ribonucleic acid in a mouse myeloma: estimation of the half-life of the messenger RNA coding for an immunoglobulin light chain
Cowan NJ; Milstein C
1974 Feb 5;82(4):469-481, Journal of molecular biology
— id: 17175, year: 1974, vol: 82, page: 469, stat: Journal Article,

Intracellular immunoglobulin chain synthesis in non-secreting variants of a mouse myeloma: detection of inactive light-chain messenger RNA
Cowan NJ; Secher DS; Milstein C
1974 Dec 25;90(4):691-701, Journal of molecular biology
— id: 17174, year: 1974, vol: 90, page: 691, stat: Journal Article,

Purification and sequence of messenger RNA for immunoglobulin light chains
Brownlee GG; Cartwright EM; Cowan NJ; Jarvis JM; Milstein C
1973 Aug 22;244(138):236-240, Nature: new biology
— id: 17176, year: 1973, vol: 244, page: 236, stat: Journal Article,

The translation in vitro of mRNA for immunoglobulin heavy chains
Cowan NJ; Milstein C
1973 Jul 2;36(1):1-7, European journal of biochemistry
— id: 17177, year: 1973, vol: 36, page: 1, stat: